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AB134102

Anti-ADO 抗体 [EPR6581]

Anti-ADO antibody [EPR6581]

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(3 Publications)

Rabbit Recombinant Monoclonal ADO antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

別名を表示する

C10orf22, ADO, 2-aminoethanethiol dioxygenase, Cysteamine dioxygenase

1 Images
Western blot - Anti-ADO antibody [EPR6581] (AB134102)
  • WB

Unknown

Western blot - Anti-ADO antibody [EPR6581] (AB134102)

Blocking/Diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-ADO antibody [EPR6581] (ab134102) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

A-673 (Human muscle Ewing's Sarcoma ) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 29 kDa

Observed band size: 28-30 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-ADO antibody [EPR6581] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR6581

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Adenosine deaminase (ADO) also known as ADA is an enzyme that plays an important role in the metabolism of purine. The molecular weight of ADO approximately 41 kDa allows it to facilitate the deamination of adenosine into inosine an essential step in nucleoside metabolism. ADO is expressed in various tissues including the lymphoid tissues and red blood cells where its enzymatic activity is necessary for maintaining cellular homeostasis.
Biological function summary

The activity of adenosine deaminase is significant for the development and function of the immune system. ADO does not act alone but is part of a larger enzymatic framework involved in nucleic acid metabolism. It helps regulate levels of adenosine and other deaminated derivatives and is important in cells where rapid proliferation occurs such as in lymphocytes. ADO interacts with proteins in this metabolic complex to manage the pool of nucleosides available to the cell which influences cell signaling processes.

Pathways

Adenosine deaminase integrates into the purine salvage pathway essential for the recycling and management of purine nucleotides in cellular metabolism. Within this framework ADO interacts with purine nucleoside phosphorylase (PNP) which further processes inosine to hypoxanthine continuing the nucleotide cycle. These processes are necessary for maintaining cellular function and survival especially under conditions that demand rapid cell proliferation or repair.

Adenosine deaminase deficiency is linked to severe combined immunodeficiency (SCID) a condition that severely impairs the immune system. This deficiency leads to the accumulation of toxic levels of adenosine and deoxyadenosine damaging lymphocytes. The enzyme's connection to diseases also includes interactions with proteins such as RAG1 and RAG2 which are important for lymphocyte development. Additionally overactivity of ADO has implications in chronic inflammatory diseases linking it to pathological conditions where purine metabolism becomes dysregulated.

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

ターゲットの情報

Plays a vital role in regulating thiol metabolism and preserving oxygen homeostasis by oxidizing the sulfur of cysteamine and N-terminal cysteine-containing proteins to their corresponding sulfinic acids using O2 as a cosubstrate (PubMed : 17581819, PubMed : 29752763, PubMed : 31273118, PubMed : 32601061). Catalyzes the oxidation of cysteamine (2-aminoethanethiol) to hypotaurine (PubMed : 17581819, PubMed : 29752763, PubMed : 32601061). Catalyzes the oxidation of regulators of G-protein signaling 4 (RGS4) and 5 (RGS5) and interleukin-32 (IL32) (PubMed : 31273118, PubMed : 32601061).
See full target information ADO

文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 31:293 PubMed41013182

2025

IRS2/FOXO1 mitigates osteoarthritis by regulating chondrocyte autophagy and mitochondrial function.

Applications

Unspecified application

Species

Unspecified reactive species

Chaoren Qin,Kai Chen,Yingchun Sun,Changjiang Wang,Yaohui Yu,Hao Zhu,Guoyou Zou

The Journal of biological chemistry 299:105156 PubMed37572852

2023

Comparative analysis of N-terminal cysteine dioxygenation and prolyl-hydroxylation as oxygen-sensing pathways in mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Min Tian,Philip Holdship,Trang Quynh To,Peter J Ratcliffe,Thomas P Keeley

Frontiers in oncology 13:1197542 PubMed37313466

2023

The pro-tumorigenic cytokine IL-32 has a high turnover in multiple myeloma cells due to proteolysis regulated by oxygen-sensing cysteine dioxygenase and deubiquitinating enzymes.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Kastnes,Kristin Roseth Aass,Siri Anshushaug Bouma,Charlotte Årseth,Muhammad Zahoor,Mariia Yurchenko,Therese Standal
View all publications

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