Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-ADIPOR1 抗体 [mAbcam50675] (ab50675)

Submit a review Q&A (1)References (3)
Western blot - Anti-ADIPOR1 antibody [mAbcam50675] (ab50675)
  • Western blot - Anti-ADIPOR1 antibody [mAbcam50675] (ab50675)

Key features and details

  • Mouse monoclonal [mAbcam50675] to ADIPOR1
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgM

製品の概要

  • 製品名

    Anti-ADIPOR1 antibody [mAbcam50675]
    ADIPOR1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [mAbcam50675] to ADIPOR1

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Zebrafish

  • 免疫原

    Synthetic peptide corresponding to Human ADIPOR1 aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab59184)

  • ポジティブ･コントロール

    • This antibody gave a positive signal in Human Skeletal Muscle Lysate.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...

  • 精製度

    Concentrated Culture Supernatant

  • 特記事項（精製）

    Tissue culture supernatant was concentrated and then buffer exchanged.

  • ポリ/モノ

    モノクローナル

  • クローン名

    mAbcam50675

  • ミエローマ

    Sp2/0-Ag14

  • アイソタイプ

    IgM

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab50675の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 10 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
特記事項
WB
Use a concentration of 10 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

ターゲット情報

  • 機能

    Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some high-affinity receptor for globular adiponectin but low-affinity receptor for full-length adiponectin.

  • 組織特異性

    Widely expressed. Highly expressed in skeletal muscle. Expressed at intermediate level in brain, heart, spleen, kidney, liver, placenta, lung and peripheral blood leukocytes. Weakly expressed in colon, thymus and small intestine.

  • 配列類似性

    Belongs to the ADIPOR family.

  • ドメイン

    The N-terminus is known to be cytoplasmic while the C-terminus is known to be extracellular.

  • 細胞内局在

    Membrane. Localized to the cell membrane and intracellular organelles.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • ACDCR1 antibody
    • ADIPO R1 antibody
    • Adiponectin receptor protein 1 antibody
    • ADIPOR 1 antibody
    • Adipor1 antibody
    • ADR1_HUMAN antibody
    • CGI 45 antibody
    • CGI 45 protein antibody
    • CGI-45 antibody
    • CGI45 antibody
    • CGI45 protein antibody
    • FLJ25385 antibody
    • FLJ42464 antibody
    • PAQR1 antibody
    • Progestin and adipoQ receptor family member I antibody
    • TESBP1A antibody
    see all

画像

  • Western blot - Anti-ADIPOR1 antibody [mAbcam50675] (ab50675)
    Western blot - Anti-ADIPOR1 antibody [mAbcam50675] (ab50675)
    Anti-ADIPOR1 antibody [mAbcam50675] (ab50675) at 10 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 20 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa
    Additional bands at: 28 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-ADIPOR1 antibody [mAbcam50675] (ab50675)
    Western blot - Anti-ADIPOR1 antibody [mAbcam50675] (ab50675)
    Anti-ADIPOR1 antibody [mAbcam50675] (ab50675) at 10 µg/ml + Human Skeletal Muscle tissue lysate at 20 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L at 1/5000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 12 minutes


    Blocking buffer: 3% Milk
    Gel type: MOPS

参考文献 (3)

ab50675 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab50675 は 3 報の論文で使用されています。

  • Wang X  et al. Schisandrin A from Schisandra chinensis Attenuates Ferroptosis and NLRP3 Inflammasome-Mediated Pyroptosis in Diabetic Nephropathy through Mitochondrial Damage by AdipoR1 Ubiquitination. Oxid Med Cell Longev 2022:5411462 (2022). PubMed: 35996380
  • Wang H  et al. 5-Hydroxytryptophan Suppresses the Abdominal Fat Deposit and Is Beneficial to the Intestinal Immune Function in Broilers. Front Physiol 11:655 (2020). PubMed: 32595527
  • Diot M  et al. Expression of adiponectin, chemerin and visfatin in plasma and different tissues during a laying season in turkeys. Reprod Biol Endocrinol 13:81 (2015). WB ; Turkey . PubMed: 26228641

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

Question

I am using ab50675 in WB and cannot detect a signal on human cell lysate colon cancer cells. The protocol advice I received to use more antibody did not help.

Read More
Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number ******. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com