Anti-ADIPOR1 抗体 (ab70362)
Key features and details
- Rabbit polyclonal to ADIPOR1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ADIPOR1 antibody
ADIPOR1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ADIPOR1 -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken, Cow, Pig, Zebrafish
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免疫原
Synthetic peptide corresponding to Human ADIPOR1 aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
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ポジティブ･コントロール
- ICC/IF: HepG2 cells treated with sodium 4-phenylbutyrate. HepG2 cells treated with valproic acid, sodium salt. HeLa and HepG2 cells. WB: Rat, mouse and human skeletal muscle tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70362の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (2) |
Use a concentration of 5 µg/ml.
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| WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
ターゲット情報
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機能
Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some high-affinity receptor for globular adiponectin but low-affinity receptor for full-length adiponectin. -
組織特異性
Widely expressed. Highly expressed in skeletal muscle. Expressed at intermediate level in brain, heart, spleen, kidney, liver, placenta, lung and peripheral blood leukocytes. Weakly expressed in colon, thymus and small intestine. -
配列類似性
Belongs to the ADIPOR family. -
ドメイン
The N-terminus is known to be cytoplasmic while the C-terminus is known to be extracellular. -
細胞内局在
Membrane. Localized to the cell membrane and intracellular organelles. - Information by UniProt
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参照データベース
- Entrez Gene: 421148 Chicken
- Entrez Gene: 407235 Cow
- Entrez Gene: 51094 Human
- Entrez Gene: 72674 Mouse
- Entrez Gene: 404695 Pig
- Entrez Gene: 289036 Rat
- Entrez Gene: 406821 Zebrafish
- Omim: 607945 Human
see all -
別名
- ACDCR1 antibody
- ADIPO R1 antibody
- Adiponectin receptor protein 1 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-ADIPOR1 antibody (ab70362)ab70362 staining ADIPOR1 in HepG2 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab70362 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Immunocytochemistry/ Immunofluorescence - Anti-ADIPOR1 antibody (ab70362)ab70362 staining ADIPOR1 in HepG2 cells treated with sodium 4-phenylbutyrate (ab141253), by ICC/IF. Increase of aADIPOR1 expression correlates with increased concentration of sodium 4-phenylbutyrate, as described in literature.
The cells were incubated at 37°C for 6 hours in media containing different concentrations of ab141253 (sodium 4-phenylbutyrate) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab70362 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. -
Western blot - Anti-ADIPOR1 antibody (ab70362)All lanes : Anti-ADIPOR1 antibody (ab70362) at 1 µg/ml
Lane 1 : Skeletal Muscle (Rat) Tissue Lysate
Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 3 : Skeletal Muscle (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 90 seconds -
Immunocytochemistry/ Immunofluorescence - Anti-ADIPOR1 antibody (ab70362)ab70362 staining ADIPOR1 in HepG2 cells treated with valproic acid, sodium salt (ab120745), by ICC/IF. Increased cytoplasmatic staining of ADIPOR1 correlates with increased concentration of valproic acid, as described in literature.
The cells were incubated at 37øC for 24h in media containing different concentrations of ab120745 (valproic acid) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab70362 (5 μg/ml) was performed overnight at 4øC in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab70362 は 17 報の論文で使用されています。
- Zhang H et al. GLIS2 Prevents Hepatic Fibrosis by Competitively Binding HDAC3 to Inhibit Hepatic Stellate Cell Activation. Cell Mol Gastroenterol Hepatol 15:355-372 (2023). PubMed: 36397300
- Dhanyalayam D et al. Sex Differences in Cardiac Pathology of SARS-CoV2 Infected and Trypanosoma cruzi Co-infected Mice. Front Cardiovasc Med 9:783974 (2022). PubMed: 35369283
- Xu N et al. Adiponectin Ameliorates GMH-Induced Brain Injury by Regulating Microglia M1/M2 Polarization Via AdipoR1/APPL1/AMPK/PPARγ Signaling Pathway in Neonatal Rats. Front Immunol 13:873382 (2022). PubMed: 35720361
- Gong Y et al. Paracrine Effects of Recombinant Human Adiponectin Promote Bone Regeneration. Front Cell Dev Biol 9:762335 (2021). PubMed: 34790669
- Choi SK et al. AdipoRon, adiponectin receptor agonist, improves vascular function in the mesenteric arteries of type 2 diabetic mice. PLoS One 15:e0230227 (2020). PubMed: 32182257