Anti-Adenylate cyclase 1/AC1 抗体 (ab69597)
Key features and details
- Rabbit polyclonal to Adenylate cyclase 1/AC1
- Suitable for: ELISA, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Adenylate cyclase 1/AC1 antibody -
製品の詳細
Rabbit polyclonal to Adenylate cyclase 1/AC1 -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, IHC-P, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69597の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 124 kDa).
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| 特記事項 |
|---|
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ELISA
Use at an assay dependent concentration. |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 124 kDa). |
ターゲット情報
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機能
This is a calmodulin-sensitive adenylyl cyclase. May be involved in regulatory processes in the central nervous system. It may play a role in memory acquisition and learning. -
組織特異性
Brain, retina and adrenal medulla. -
配列類似性
Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
Contains 2 guanylate cyclase domains. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 107 Human
- Entrez Gene: 432530 Mouse
- Omim: 103072 Human
- SwissProt: Q08828 Human
- SwissProt: O88444 Mouse
- Unigene: 192215 Human
- Unigene: 259733 Mouse
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別名
- Adenylate cyclase 1, fetal brain, type I antibody
- 3',5'-cyclic AMP synthetase antibody
- AC1 antibody
see all
画像
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Western blot - Anti-Adenylate cyclase 1/AC1 antibody (ab69597)All lanes : Anti-Adenylate cyclase 1/AC1 antibody (ab69597) at 1/500 dilution
Lane 1 : Extracts from COLO205
Lane 2 : Extracts from COLO205 with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 124 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Additional bands at: 105 kDa, 150 kDa, 85 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenylate cyclase 1/AC1 antibody (ab69597)IHC image of ab69597 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab69597, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab69597 は 6 報の論文で使用されています。
- Liu RH et al. Inhibiting neuronal AC1 for treating anxiety and headache in the animal model of migraine. iScience 26:106790 (2023). PubMed: 37235050
- Zhang WW et al. Peripheral ablation of type III adenylyl cyclase induces hyperalgesia and eliminates KOR-mediated analgesia in mice. JCI Insight 7:N/A (2022). PubMed: 34914639
- Zhang X et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics 11:5650-5674 (2021). PubMed: 33897873
- Liu SB et al. Cyclic AMP-dependent positive feedback signaling pathways in the cortex contributes to visceral pain. J Neurochem 153:252-263 (2020). PubMed: 31665810
- Peng J et al. Single-cell transcriptomes reveal molecular specializations of neuronal cell types in the developing cerebellum. J Mol Cell Biol 11:636-648 (2019). PubMed: 30690467
- Tekmen M & Gleason E Multiple Ca2+-dependent mechanisms regulate L-type Ca2+ current in retinal amacrine cells. J Neurophysiol 104:1849-66 (2010). WB ; Chicken . PubMed: 20685929