AB69597

Anti-Adenylate cyclase 1/AC1 抗体

Anti-Adenylate cyclase 1/AC1 antibody

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(8 Publications)

Rabbit Polyclonal Adenylate cyclase 1/AC1 antibody. Suitable for IHC-P, ELISA, WB and reacts with Human, Mouse samples. Cited in 8 publications.

別名を表示する

Adenylate cyclase type 1, ATP pyrophosphate-lyase 1, Adenylate cyclase type I, Adenylyl cyclase 1, Ca(2+)/calmodulin-activated adenylyl cyclase, ADCY1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenylate cyclase 1/AC1 antibody (AB69597)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenylate cyclase 1/AC1 antibody (AB69597)

IHC image of ab69597 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab69597, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Unknown

Western blot - Anti-Adenylate cyclase 1/AC1 antibody (AB69597)

All lanes:

Western blot - Anti-Adenylate cyclase 1/AC1 antibody (ab69597) at 1/500 dilution

Lane 1:

Extracts from COLO205 at 5 µg

Lane 2:

Extracts from COLO205 at 5 µg with immunising peptide

Predicted band size: 123 kDa

Observed band size: 105 kDa,130 kDa,150 kDa,85 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human, Mouse

アプリケーション

WB, ELISA, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Adenylate cyclase 1 (AC1) also known as adenylate cyclase is an enzyme that catalyzes the conversion of ATP to cyclic AMP (cAMP) in response to various signaling molecules. This enzyme has a molecular mass of approximately 130 kDa. AC1 is localized mainly in neurons of the central nervous system. It acts as an adenylate cyclase activator responding to signals and modulating the downstream effects via cAMP production. Forskolin is a well-known pharmacological activator of adenylate cyclases including AC1 which can elevate intracellular cAMP levels.

Biological function summary

Adenylate cyclases like AC1 play critical roles in signal transduction by regulating the intracellular concentration of cAMP a second messenger involved in numerous cellular processes. AC1 is part of a complex including G-protein coupled receptors which regulate its activity. The presence of AC1 in the brain suggests its involvement in neural signaling and neuroplasticity contributing to the modulation of synaptic transmission and the function of neuronal networks.

Pathways

Adenylate cyclase activity is integral in the cAMP signaling pathway where it mediates the effects of neurotransmitters and hormones. This pathway is important for processes such as learning and memory where cAMP acts as an important mediator. AC1 activity is linked to proteins involved in the Calcium/Calmodulin-dependent protein kinase pathway providing a bridge between calcium signaling and cAMP production which influences synaptic plasticity and neurocognitive functions.

AC1 has connections to conditions like depression and addiction where dysregulation of cAMP signaling is observed. Altered activity of AC1 can affect other proteins such as protein kinase A which plays a role in emotional and reward responses. Understanding the function of AC1 in these conditions aids in exploring potential therapeutic targets for neuropsychiatric and neurodegenerative diseases.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Catalyzes the formation of the signaling molecule cAMP in response to G-protein signaling. Mediates responses to increased cellular Ca(2+)/calmodulin levels (By similarity). May be involved in regulatory processes in the central nervous system. May play a role in memory and learning. Plays a role in the regulation of the circadian rhythm of daytime contrast sensitivity probably by modulating the rhythmic synthesis of cyclic AMP in the retina (By similarity).

See full target information Adenylate cyclase type 1

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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

The journal of headache and pain 25:81 PubMed38760739

2024

Alleviation of migraine related pain and anxiety by inhibiting calcium-stimulating AC1-dependent CGRP in the insula of adult rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Chenhao Li,Qi-Yu Chen,Shun Hao,Jingrui Mao,Wenwen Zhang,Xun Han,Zhao Dong,Ruozhuo Liu,Wenjing Tang,Min Zhuo,Shengyuan Yu,Yinglu Liu

iScience 26:106790 PubMed37235050

2023

Inhibiting neuronal AC1 for treating anxiety and headache in the animal model of migraine.

Applications

Unspecified application

Species

Unspecified reactive species

Ren-Hao Liu,Mingjie Zhang,Man Xue,Tao Wang,Jing-Shan Lu,Xu-Hui Li,Yu-Xin Chen,Kexin Fan,Wantong Shi,Si-Bo Zhou,Qi-Yu Chen,Li Kang,Qian Song,Shengyuan Yu,Min Zhuo

JCI insight 7: PubMed34914639

2022

Peripheral ablation of type III adenylyl cyclase induces hyperalgesia and eliminates KOR-mediated analgesia in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Wen Zhang,Hong Cao,Yang Li,Xian-Jun Fu,Yu-Qiu Zhang

Theranostics 11:5650-5674 PubMed33897873

2021

Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Zhang,Keke Yu,Lifang Ma,Zijun Qian,Xiaoting Tian,Yayou Miao,Yongjie Niu,Xin Xu,Susu Guo,Yueyue Yang,Zhixian Wang,Xiangfei Xue,Chuanjia Gu,Wentao Fang,Jiayuan Sun,Yongchun Yu,Jiayi Wang

Molecular pain 16:1744806920972241 PubMed33243040

2020

FMRP acts as a key messenger for visceral pain modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Liu-Kun Yang,Liang Lu,Ban Feng,Xin-Shang Wang,Jiao Yue,Xu-Bo Li,Min Zhuo,Shui-Bing Liu

Journal of neurochemistry 153:252-263 PubMed31665810

2020

Cyclic AMP-dependent positive feedback signaling pathways in the cortex contributes to visceral pain.

Applications

Unspecified application

Species

Unspecified reactive species

Shui-Bing Liu,Xin-Shang Wang,Jiao Yue,Le Yang,Xu-Hui Li,Li-Ning Hu,Jing-Shan Lu,Qian Song,Kun Zhang,Qi Yang,Ming-Ming Zhang,Matteo Bernabucci,Ming-Gao Zhao,Min Zhuo

Journal of molecular cell biology 11:636-648 PubMed30690467

2019

Single-cell transcriptomes reveal molecular specializations of neuronal cell types in the developing cerebellum.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Peng,Ai-Li Sheng,Qi Xiao,Libing Shen,Xiang-Chun Ju,Min Zhang,Si-Ting He,Chao Wu,Zhen-Ge Luo

Journal of neurophysiology 104:1849-66 PubMed20685929

2010

Multiple Ca2+-dependent mechanisms regulate L-type Ca2+ current in retinal amacrine cells.

Applications

Species

Chicken

Merve Tekmen,Evanna Gleason

View all publications

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Abcam product promise

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For licensing inquiries, please contact partnerships@abcam.com

Anti-Adenylate cyclase 1/AC1 抗体

Anti-Adenylate cyclase 1/AC1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenylate cyclase 1/AC1 antibody (AB69597)

Western blot - Anti-Adenylate cyclase 1/AC1 antibody (AB69597)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (8)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise