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Anti-Adenosine Receptor A2a 抗体 [7F6-G5-A2] (ab79714)

Reviews (3)Q&A (6)References (9)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714)
  • Western blot - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714)

Key features and details

  • Mouse monoclonal [7F6-G5-A2] to Adenosine Receptor A2a
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-Adenosine Receptor A2a antibody [7F6-G5-A2]
    Adenosine Receptor A2a 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [7F6-G5-A2] to Adenosine Receptor A2a

  • 由来種

    Mouse

  • 特異性

    This antibody is recommended for tissue lysates only. In house testing has shown no signal in Western Blot for SH-SY5Y, SK-N-SH, PC-12 or HeLa cell lines.

  • アプリケーション

    適用あり: IHC-P, WBmore details
    適用なし: ICC/IF

  • 種交差性

    交差種: Mouse, Rat, Human
    交差が予測される動物種: Guinea pig, Hamster, Dog, Non human primates

  • 免疫原

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • エピトープ

    Recognises amino acids 213-220 (SQPLPGER) within the third intracellular loop.

  • ポジティブ･コントロール

    • WB: Human, mouse, and rat brain tissue lysates. IHC-P: Rat, mouse and human brain caudate nucleus.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab79714の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (1)
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa).

We recommend using 1% BSA as a blocking agent for western blot.
特記事項
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa).

We recommend using 1% BSA as a blocking agent for western blot.
追加情報
Is unsuitable for ICC/IF.

ターゲット情報

  • 機能

    Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.

  • 配列類似性

    Belongs to the G-protein coupled receptor 1 family.

  • ドメイン

    The cytoplasmic C-terminal domain is necessary for targeting the non-ubiquitinated form of this protein to the cell surface.

  • 翻訳後修飾

    Ubiquitinated. Deubiquitinated by USP4; leading to stabilization and expression at the cell surface.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • A2AAR antibody
    • A2aR antibody
    • AA2AR_HUMAN antibody
    • ADENO antibody
    • Adenosine A2 receptor antibody
    • Adenosine A2a receptor antibody
    • Adenosine receptor A2a antibody
    • Adenosine receptor subtype A2a antibody
    • ADORA 2 antibody
    • ADORA2 antibody
    • ADORA2A antibody
    • hA2aR antibody
    • RDC 8 antibody
    • RDC8 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714)

    IHC image of Adenosine Receptor A2a staining in Mouse normal brain Caudate Nucleus formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79714, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714)
    Western blot - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714)
    Lanes 1-3 : Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714) at 5 µg/ml
    Lanes 4-6 : Competitor product at 5 µg/ml

    Lanes 1 & 4 : Human brain tissue lysate
    Lanes 2 & 5 : Mouse brain tissue lysate
    Lanes 3 & 6 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa
    Additional bands at: 25 kDa, 39 kDa, 55 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    Blocking buffer: 1% BSA

    Gel type: MOPS

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714)

    IHC image of Adenosine Receptor A2a staining in Human normal brain Caudate Nucleus formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79714, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714)

    IHC image of Adenosine Receptor A2a staining in Rat normal brain Caudate Nucleus formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79714, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

参考文献 (9)

ab79714 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab79714 は 9 報の論文で使用されています。

  • Ran L  et al. ADORA2A promotes proliferation and inhibits apoptosis through PI3K/AKT pathway activation in colorectal carcinoma. Sci Rep 13:19477 (2023). PubMed: 37945707
  • Muñoz-López S  et al. Resveratrol enhances A1 and hinders A2A adenosine receptors signaling in both HeLa and SH-SY5Y cells: Potential mechanism of its antitumoral action. Front Endocrinol (Lausanne) 13:1007801 (2022). PubMed: 36407311
  • Wang X  et al. Caffeine reduces oxidative stress to protect against hyperoxia-induced lung injury via the adenosine A2A receptor/cAMP/PKA/Src/ERK1/2/p38MAPK pathway. Redox Rep 27:270-278 (2022). PubMed: 36357965
  • Stocco E  et al. Experimental Evidence of A2A-D2 Receptor-Receptor Interactions in the Rat and Human Carotid Body. Front Physiol 12:645723 (2021). PubMed: 33935801
  • Sánchez-Melgar A  et al. Adenosine and Metabotropic Glutamate Receptors Are Present in Blood Serum and Exosomes from SAMP8 Mice: Modulation by Aging and Resveratrol. Cells 9:N/A (2020). PubMed: 32645849
  • Huang W  et al. An adenosine A1R-A2aR imbalance regulates low glucose/hypoxia-induced microglial activation, thereby contributing to oligodendrocyte damage through NF-?B and CREB phosphorylation. Int J Mol Med 41:3559-3569 (2018). PubMed: 29512780
  • Sakano D  et al. Dopamine D2 Receptor-Mediated Regulation of Pancreatic ß Cell Mass. Stem Cell Reports 7:95-109 (2016). Mouse . PubMed: 27373926
  • Niesman IR  et al. Traumatic brain injury enhances neuroinflammation and lesion volume in caveolin deficient mice. J Neuroinflammation 11:39 (2014). WB ; Mouse . PubMed: 24593993
  • Bone DB  et al. Nucleoside/nucleobase transport and metabolism by microvascular endothelial cells isolated from ENT1-/- mice. Am J Physiol Heart Circ Physiol 299:H847-56 (2010). WB ; Mouse . PubMed: 20543083

レビューと Q&A

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1-9 of 9 Abreviews or Q&A

Flow Cytometry abreview for Anti-Adenosine Receptor A2a antibody [7F6-G5-A2]

 Good
Abreviews
abreview image
Application
Flow Cytometry
Sample
Human Cell (BLOOD)
Permeabilization
Yes - Staining Perm Wash Buffer bioLegend
Gating Strategy
cells were also labeled with CD4 antigene. after FSC/SSC appropriate for white blood cells
Specification
BLOOD
Fixation
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 12 2022

Western blot

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8)
Sample
Human Tissue lysate - whole (Heart)
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jun 10 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-Adenosine Receptor A2a antibody [7FG-G5-A2]

 Good
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HepG2)
Specification
HepG2
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS containing 0.2% Triton X100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 18 2011

Question

Hi,

Our customer has responded with the following;

In regards to the query from Abcam the secondary antibody that we have been using to detect A2A using ab79714 is an Alexa Fluor 700 Goat-anti-mouse IgG2 (H+L) from xxxxx

If we are able to get a replacement for this using the alternative ab101678 we will be using Alexa Fluor 700 Goat-anti-rabbit IgG2 (H+L) from xxxxx

The good news is the replacement antibody for the mGluR5 is working perfectly on human brn extracts and we are detecting a single band at 140 kDa.

Please let me know if you have any further questions.

Looking forward to your response.

Read More
Answer

Thank you for that reply.

Kate is currently away from the office but I will try to help as best I can with your enquiry. I have checked the two secondary antibodies intended to be used and they seem like they should perform fine with the primary antibodies. As Kate has indicated, I would be happy to provide the alternative antibody ab101678 if your customer would like.

Could you please just confirm I have the correct order number:
xxxx

Then I will arrange for the replacement to be sent to you as soon as possible.

I look forward to receiving your reply.

Read More

Question

I hope you have been well

Our customer has responded with the following;

I have attached the latest results for A2A antibody using proteins isolated using the suggested RIPA buffer. Unfortunately we still cannot detect A2A even though our positive control (B-actin) worked in the same samples.

Would it be possible to get a replacement for this antibody? If so, could we exchange it for Anti-Adenosine Receptor A2a antibody (ab101678) instead as we are putting in an order for the Anti-Adenosine Receptor A2a protein (Tagged)(ab131999) and this is the antibody that Abcam recommends to be used for detection.

I will send you the results from this new antibody as soon as we have the data.

Please feel free to contact me should you require any information.

Hoping to hear back from you soon.

Kind regards,

Read More
Answer

Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments.

Before proceeding, could you confirm the secondary antibody used will detect mouse IgG2a isotype? What is the supplier and product number?

If ths antibody is not working with the correct secondary, I would be pleased to arrange an alternative replacement as requested.

I look forward to hearing from you.

Read More

Question

Thank you for your patience.

Our customer has responded with the following;

In regard to your questions:

1. My mistake I actually meant we used an anti-mouse secondary not anti-rabbit.

2. I will run our next lot of samples using both our standard buffer and the RIPA buffer and will email you the results once we try them with the new antibodies.

3. We can routinely detect ng quantities of both GAPDH and b-actin as our controls - so we did not think it was anything to do with sample preparation.

Looking forward to your response.

Kind regards,

Read More
Answer

Thank you for your message and for kindly confirming these details.

I look forward to hearing from you with the results from the samples lysed in RIPA. Please do not hesitate to let me know how you get on. If this still does not work with RIPA, I'll be pleased to arrange a free of charge replacement or credit note.

I hope this will be helpful.

Read More

Question

Lot number: GR80530-1

Inquiry: Hi Technical, We have a customer who has experiencing problems with this antibody. See attached a questionnaire. Kind regards

Read More
Answer

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

I would like to reassure you that ab79714 is tested and covered by our 6 month guarantee for use in WB and human samples. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement.

Reviewing this case, I would like to offer some suggestions to help optimize the results. I would also appreciate if you can confirm some further details:

1. I notice the primary antbody ab79714 is a mouse monoclonal IgG2a antibody. The secondary antibody mentioned on the form is goat anti rabbit, which will not detect the mouse primary. I can suggest to use a secondary antibody which is anti mouse IgG, or anti mouse IgG2a.

2. I can recommend to try RIPA lysis buffer to prepare the samples. This may provide amore suitable protein preparation.

3. What were the results from the loading control (GAPDH and b actin)?

I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

Read More

Question

Hi



Could I please have a quote for this product please, so I can send it along to our orders department.



Thanks

Read More
Answer

Thank you for your reply.

I can confirm that I send you the quote for ab79714 in a separate email.

Read More

Question

Product code: 79714

Inquiry: Hi I'm a PhD student as Aston University, and our lab is interested in changing our HIS antibodies used in conjunction with our hA2aR proteins to your Ab's that target the protein instead of its tag epitope. And we were wondering if you could send us a sample to check if they work with our protein. Thanks

Read More
Answer

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

We like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

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