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Anti-Adenosine A1 Receptor 抗体 (ab82477)

Reviews (2)Q&A (9)References (31)
Western blot - Anti-Adenosine A1 Receptor antibody (ab82477)
  • Immunocytochemistry/ Immunofluorescence - Anti-Adenosine A1 Receptor antibody (ab82477)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine A1 Receptor antibody (ab82477)

Key features and details

  • Rabbit polyclonal to Adenosine A1 Receptor
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Adenosine A1 Receptor antibody
    Adenosine A1 Receptor 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Adenosine A1 Receptor

  • 由来種

    Rabbit

  • 特異性

    Replenishment batches of ab82477 are tested in WB. Previous batches were additionally validated in ICC/IF and IHC-P. These applications are still expected to work and are covered by our Abpromise guarantee.

  • アプリケーション

    適用あり: IHC-P, WB, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Rat

  • 免疫原

    Synthetic peptide corresponding to Rat Adenosine A1 Receptor aa 300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab91076)

  • ポジティブ･コントロール

    • This antibody gave a positive signal in Rat Spinal Ganglion Neuronal cell lysate and Mouse EL4 Lymphoma whole cell lysate.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab82477の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (1)
Use a concentration of 1 µg/ml.
WB (1)
Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
ICC/IF
Use a concentration of 5 µg/ml.
特記事項
IHC-P
Use a concentration of 1 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
ICC/IF
Use a concentration of 5 µg/ml.

ターゲット情報

画像

  • Western blot - Anti-Adenosine A1 Receptor antibody (ab82477)
    Western blot - Anti-Adenosine A1 Receptor antibody (ab82477)
    All lanes : Anti-Adenosine A1 Receptor antibody (ab82477) at 1 µg/ml

    Lane 1 : Rat Spinal Ganglion Neuronal cell lysate
    Lane 2 : EL4 whole cell lysate (ab7183)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 36 kDa
    Observed band size: 36 kDa
    Additional bands at: 118 kDa, 18 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute
  • Immunocytochemistry/ Immunofluorescence - Anti-Adenosine A1 Receptor antibody (ab82477)
    Immunocytochemistry/ Immunofluorescence - Anti-Adenosine A1 Receptor antibody (ab82477)
    ICC/IF image of ab82477 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82477, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) PC12 cells at 5µg/ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine A1 Receptor antibody (ab82477)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine A1 Receptor antibody (ab82477)
    IHC image of ab82477 staining in Rat Adult Brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82477, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

参考文献 (31)

ab82477 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab82477 は 31 報の論文で使用されています。

  • Wu Z  et al. Dietary adenosine supplementation improves placental angiogenesis in IUGR piglets by up-regulating adenosine A2a receptor. Anim Nutr 13:282-288 (2023). PubMed: 37168450
  • Mukai A  et al. Protective effects of remote ischemic preconditioning against spinal cord ischemia-reperfusion injury in rats. J Thorac Cardiovasc Surg 163:e137-e156 (2022). PubMed: 32414598
  • Zhang AQ  et al. Silencing miRNA-324-3p protects against cerebral ischemic injury via regulation of the GATA2/A1R axis. Neural Regen Res 17:2504-2511 (2022). PubMed: 35535903
  • Guiol C  et al. Adenosine Receptors Profile in Fibromuscular Dysplasia. Biomedicines 10:N/A (2022). PubMed: 36359350
  • Lezmy J  et al. Astrocyte Ca2+-evoked ATP release regulates myelinated axon excitability and conduction speed. Science 374:eabh2858 (2021). PubMed: 34648330
View all Publications for this product

レビューと Q&A

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1-10 of 11 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Adenosine A1 Receptor antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA
Sample
Human Tissue sections (Brain cortex)
Specification
Brain cortex
Permeabilization
Yes - TBS-T (0.05% tween)
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 28 2014

Western blot abreview for Anti-Adenosine A1 Receptor antibody

 Below Average
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-20% or 10%)
Sample
Human Cell lysate - whole cell (CHO-cells)
Specification
CHO-cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. David Wilkes

Verified customer

投稿 May 11 2012

Question

ab82477: used with CHO and human cells (AD293) - both untested
sees band although RT-PCR shows no signal and samples should be negative
ab124780: used with AD293 cells (human tested)
also sees bands although RT-PCR shows no signal and samples should be negative
but 293T cells give nice band in WB on datasheet
suggestion: try ab82477 on mouse or rat which are tested species

Read More
Answer

Thank you for contacting us.
I am sorry to hear that the 2 antibodies do not seem to work as expected.

As we discussed over the phone, ab82477 has not been tested in human and chinese hamster samples. But if you could try it on mouse or rat samples (which are tested species), we could come to a better conclusion regarding the performance of the antibody.

As for ab124780, we did obtain a band for 293T cells and the antibody should give a band for human samples. Would you be able to test 293T cells, also for the RT-PCR signal?

I look forward to hear back from you and assist you further.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Question

I apologize for the delay in replying. We have received a new antibody https://www.abcam.com/Adenosine-A1-Receptor-antibody-EPR6179-ab124780.html, and in the process of optimizing it. I will let you know our results and give some more feedback on ab82477.

Read More
Answer

Thank you so much for your reply.

I look forward to hear back from you and wish you good luck with ab124780. This antibodyhas been tested in WB with human samples, and thus, will be guaranteed.

Read More

Question

ReviewID: xxx AbID: 82477, Anti-Adenosine A1 Receptor antibody Rating: Below Average Image: Anti-Adenosine A1 Receptor antibody for Western blot (Human) Sample: Species: Human Type: Cell lysate - whole cell Loading amount: 20 µg Specification: CHO-cells Application: Application: Gel Running Conditions: Reduced Denaturing (4-20% or 10%) Blocking step: BSA as blocking agent for 1 hour at RT°C Blocking Concentration: 5% Other detail: Dilution: 1/200 Incubation time: 12 hours at 4°C Diluent: 5% BSA Secondary Antibody: Name: Non-Abcam Antibody was used: Donkey Anti-Rabbit IgG Conjugation: HRP Dilution: 1/20000 Detection: Detection method: ECL+ Exposure: 5 minutes and 0 seconds Bands: Specific: 37 kDa Non-specific: 60 kDa Positive Control: CHO-AR, line #2 Negative Control: CHO-cell line #1 Additional Data:

Read More
Answer

Thank you for submitting an Abreview of ab82477. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a untested species, we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.

Based on the information in your review, I feel that altering the following step in your protocol may help improve your results:

- try the primary antibody on mouse or rat samples as positive control
- load more human protein
- check the secondary antibody

If you decide to follow these suggestions, please let me know if they are helpful.

I look forward to your reply.

Read More

Question

Product code: 82477

Inquiry: I am doing Ph.D at groningen university, UMCG., dept Nuclear medicine and molecular Imaging.I would like to order the adenosine A1 antibody to perform Immunohistochemistry.If i understand properly, ab82477 , this product has been performed immunohistochemistry on paraffin embedded based.But I want to Perform paraffin as well as frozen based too. I would like to have the adenosine A1 Immunohistochemistry protocol (paraffin based) . I will be heplful if you can provide me the protocol and also I like to order the product. I want to perform the immunohistochemistry on rat's brain. Awaiting for your kind response. Thanks in advance!! Kind Regards

Read More
Answer

Thank you very much for your interest in ab82477.

This antibody was tested in IHC-P from our lab using Leica BondTM system with the standard protocol F. If you do not have access to this system I suggest to use a standard protocol that can be found here:
https://www.abcam.com/index.html?pageconfig=resource&rid=11384

https://www.abcam.com/index.html?pageconfig=resource&rid=13046

The antigen retrieval used to generate the image on the datasheet was Citrate buffer pH6 for 20min. I suggest to incubate the antibody overnight at 4C to achieve the best saturation.

To our knowledge, ab82477 has not been tested in IHC-Fr. Therefore, I can offer a discount off a future purchase if you buyab82477 now, test it in IHC-Fr and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody or the value of your next order..

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and testab82477 in IHC-Fr. I will then send a discount code. This code must be issued before purchasing ab82477 so please wait for my reply before ordering.

2. Purchase ab82477 either by phone, fax, or online (www.abcam.com).

3. Test it in IHC-Fr.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyproduct ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab82477 turns out to be unsuitable for IHC-Fr, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Question

I would like to test ab82477 for use on human samples.

Read More
Answer

DISCOUNT CODE: ***
Expiration date: August 4th, 2012

I am very pleased to hear you would like to accept our offer and test ab82477 on human samples. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for human and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Question

Searching for Adenosine receptor 1 antibody tested in IHC-P, IHC-Fr and rat.
With IHC protocol

Read More
Answer

Thank you for your telephone enquiry this morning.

As discussed on the telephone, I can confirm we do have a Adenosine A1 Receptor antibody tested in rat and IHC-P:

ab82477 Adenosine A1receptor antibody

However, I am sorry that to our knowledge, ab82477 and our other Adenosine A1 receptor antibodies have not been tested in IHC-Fr yet.

Therefore, I can offer a discount off a future purchase if you buy a82477b now, test it in IHC-Fr and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab82477 in IHC-Fr. I will then send a discount code. This code must be issued before purchasing ab82477 so please wait for my reply before ordering.

2. Purchase ab82477 either by phone, fax, or online (www.abcam.com).

3. Test it in IHC-Fr.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab82477 turns out to be unsuitable for IHC-Fr, you will still receive the discount on your next purchase after your Abreview has been submitted.

With regards to the protocol, this particular antibody is tested using an automated system, and so the protocol would not be relevant to other laboratories. I can suggest to review our immunostaining protocols from the protocols page. This provides protocol guidelines, however we recommend that procedures will often require individual optimization by the end user:
https://www.abcam.com/index.html?pageconfig=popular_protocols

Please let me know if you have any questions about the protocol or theoffer and I would be happy to help you further.

The Terms and Conditions of the testingoffer can be found at: www.abcam.com/collaborationdiscount.

Read More

Question

Dear Technical Team, Hope this e-mail finds you well! Kindly arrange to send us the certificate of compliance (CoC) for the below Abcam products as desired by one of our customers : Cat No. Description ab97021 Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin) ab97049 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Biotin) ab97023 Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) ab60314 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Chromeo™ 488) ab97080 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed ab65623 ECL Western Blotting Substrate Kit - 50 Tests ab52476 Rabbit monoclonal [EP1829Y] to CaMKII ab11800 Goat polyclonal to DCXR ab7260 Rabbit polyclonal to GFAP - Astrocyte Marker ab104224 Mouse monoclonal [1B7] to FOX3 ab76260 Rabbit monoclonal [EP2162Y] to Synapsin I (phospho S9) ab64230 Mounting Medium ab82477 Rabbit polyclonal to Adenosine A1 Receptor ab3462 Rabbit polyclonal to alpha 1 Adrenergic Receptor

Read More
Answer

I am beginning to gather the CoC's you have requested. When you have a chance, please provide the lot number from each catalog number so I can include this information on the documentation. Thanks for the additional information.

Read More

Question

Could you please send me a copy of the blot image that you ran for both the antibodies for me to compare?

Read More
Answer

I attached the WB for ab82477. The first lane is rat spinal ganglion neuronal cell lysate. The second lane is the positive control- ab7183. Both antibody westerns can be found on the datasheet on the abcam website. Good luck.

Read More

1-10 of 11 Abreviews or Q&A

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