AB3460

Anti-Adenosine A1 Receptor 抗体

Name: Anti-Adenosine A1 Receptor 抗体 (ab3460) | Abcam
Brand: Abcam Limited
SKU: AB3460
Price: 76000 JPY
Availability: InStock

Anti-Adenosine A1 Receptor antibody

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(8 Publications)

Rabbit Polyclonal Adenosine A1 Receptor antibody. Suitable for ICC, WB, IHC-P and reacts with Human, Rat samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Rat Adora1 aa 300 to C-terminus.

別名を表示する

Adenosine receptor A1

2 Images

Supplier Data

Immunocytochemistry - Anti-Adenosine A1 Receptor antibody (AB3460)

Immunocytochemical analysis of 4% paraformaldehyde-fixed, 0.1% Triton™ X-100 permeabilized HeLa cells staining adenosine A1 receptor with ab3460 at 2 μg/ml and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution (green). Nuclei are stained with SlowFade® Gold Antifade Mountant with DAPI (blue) and F-actin is stained with Alexa Fluor® 555 Rhodamine Phalloidin (red). Negative control contains no primary anibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine A1 Receptor antibody (AB3460)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine A1 Receptor antibody (AB3460)

ab3460 labelling Adenosine A1 receptor in the cytoplasm of Rat brain tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1 : 100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Rat, Human

アプリケーション

IHC-P, ICC, WB

applications

免疫原

Synthetic Peptide within Rat Adora1 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P25099

特異性

Detects Adenosine Receptor A1. This antibody does not detect other AR subtypes.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "2 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "" }, "Cow": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The Adenosine A1 Receptor also called A1 adenosine receptor is a G protein-coupled receptor (GPCR) with a mass of approximately 37 kDa. This receptor is primarily responsible for binding adenosine a nucleoside involved in numerous physiological processes. Mechanically the A1 receptor inhibits adenylyl cyclase activity via the Gi/o proteins resulting in decreased intracellular cyclic AMP levels. This receptor is expressed in various tissues including the brain heart and kidneys indicating its widespread physiological roles.

Biological function summary

The A1 receptor plays a significant role in mediating the inhibitory effects of adenosine on neurotransmitter release. It regulates synaptic transmission modulates excitability and protects tissues from excessive stimulation. This receptor does not function alone but is part of complex networks interacting with other proteins such as A2A receptors. A1 adenosine receptor signaling contributes to cardioprotection neuroprotection and regulation of renal blood flow reflecting its importance in maintaining homeostasis.

Pathways

The A1 receptor is essential in the adenosine signaling pathway and myocardial protection pathway. Through these pathways it influences important physiological responses like heart rate and blood flow. It interacts with proteins such as adenosine kinase and adenosine deaminase which regulate adenosine concentration influencing the availability of adenosine for receptor binding. The pathways involved illustrate the receptor's role in balancing energy supply and demand.

The A1 adenosine receptor is implicated in conditions like cardiac arrhythmias and epilepsy. Its role in reducing excitability and modulating synaptic transmission makes it a potential target for epilepsy treatment. In cardiac arrhythmias it helps regulate heart rate and protects against ischemic damage. The receptor's interactions with A2B receptors emphasize its role in complex disease mechanisms where it balances excitatory and inhibitory signals providing opportunities for therapeutic interventions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Receptor for adenosine. The activity of this receptor is mediated by G proteins such as GNAI2 which inhibit adenylyl cyclase (By similarity).

See full target information Adora1

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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1545780 PubMed40376586

2025

Inhibiting ADORA1 enhances glioma apoptosis and increases its sensitivity to anti-PD1 therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Jiang Li,Zhi-Yun Yu,Hua-Ping Gao,Yi-Ran Xu,Xue-Yuan Li,Wei Jiang,Di Chen,Dong-Ming Yan,Chao Yang,Xian-Zhi Liu

Cell discovery 10:81 PubMed39103336

2024

40 Hz light flickering facilitates the glymphatic flow via adenosine signaling in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoting Sun,Liliana Dias,Chenlei Peng,Ziyi Zhang,Haoting Ge,Zejun Wang,Jiayi Jin,Manli Jia,Tao Xu,Wei Guo,Wu Zheng,Yan He,Youru Wu,Xiaohong Cai,Paula Agostinho,Jia Qu,Rodrigo A Cunha,Xuzhao Zhou,Ruiliang Bai,Jiang-Fan Chen

EMBO reports 25:2610-2634 PubMed38698221

2024

Synaptotagmin-11 facilitates assembly of a presynaptic signaling complex in post-Golgi cargo vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Luca Trovò,Stylianos Kouvaros,Jochen Schwenk,Diego Fernandez-Fernandez,Thorsten Fritzius,Pascal Dominic Rem,Simon Früh,Martin Gassmann,Bernd Fakler,Josef Bischofberger,Bernhard Bettler

Neuropathology and applied neurobiology 49:e12926 PubMed37483117

2023

Ectopic expression of neuronal adenosine kinase, a biomarker in mesial temporal lobe epilepsy without hippocampal sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyi Guo,Jing Wang,Zhonghua Xiong,Xiongfei Wang,Yujiao Yang,Yifan Zhang,Chongyang Tang,Jing Zhang,Yuguang Guan,Fan Chen,Kun Yao,Pengfei Teng,Jian Zhou,Feng Zhai,Detlev Boison,Guoming Luan,Tianfu Li

Molecular neurobiology 56:797-811 PubMed29797183

2018

Adenosine A-Dopamine D Receptor Heteromers Control the Excitability of the Spinal Motoneuron.

Applications

Unspecified application

Species

Unspecified reactive species

Marla Rivera-Oliver,Estefanía Moreno,Yocasta Álvarez-Bagnarol,Christian Ayala-Santiago,Nicole Cruz-Reyes,Gian Carlo Molina-Castro,Stefan Clemens,Enric I Canela,Sergi Ferré,Vicent Casadó,Manuel Díaz-Ríos

Development (Cambridge, England) 139:215-24 PubMed22147955

2011

Engrailed homeoprotein recruits the adenosine A1 receptor to potentiate ephrin A5 function in retinal growth cones.

Applications

Unspecified application

Species

Unspecified reactive species

Olivier Stettler,Rajiv L Joshi,Andrea Wizenmann,Jürgen Reingruber,David Holcman,Colette Bouillot,François Castagner,Alain Prochiantz,Kenneth L Moya

Circulation 121:963-72 PubMed20159830

2010

Mechanisms by which adenosine restores conduction in dormant canine pulmonary veins.

Applications

Species

Dog

Tomás Datino,Laurent Macle,Xiao-Yan Qi,Ange Maguy,Philippe Comtois,Denis Chartier,Peter G Guerra,Angel Arenal,Francisco Fernández-Avilés,Stanley Nattel

American journal of physiology. Renal physiology 295:F108-17 PubMed18448594

2008

Acute inhibition of the betaine transporter by ATP and adenosine in renal MDCK cells.

Applications

Species

Dog

Stephen A Kempson,Jason M Edwards,Alyssa Osborn,Michael Sturek

View all publications

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Abcam product promise

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For licensing inquiries, please contact partnerships@abcam.com

Anti-Adenosine A1 Receptor 抗体

Anti-Adenosine A1 Receptor antibody

Immunocytochemistry - Anti-Adenosine A1 Receptor antibody (AB3460)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine A1 Receptor antibody (AB3460)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (8)

Abcam product promise