Anti-ADAR1 抗体 [EPR24438-41] - BSA and Azide free (Detector) (ab302751)
![Sandwich ELISA - Anti-ADAR1 antibody [EPR24438-41] - BSA and Azide free (Detector) (ab302751)](https://www.abcam.co.jp/ps/products/302/ab302751/Images/ab302751-2-human-adar1-antibody-pair-sandwich-elisa-standard-curve-2.jpg "Sandwich ELISA - Anti-ADAR1 antibody [EPR24438-41] - BSA and Azide free (Detector) (ab302751)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24438-41] to ADAR1
- Suitable for: Sandwich ELISA
- Reacts with: Human
製品の概要
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製品名
Anti-ADAR1 antibody [EPR24438-41] - BSA and Azide free (Detector)
ADAR1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR24438-41] to ADAR1 -
由来種
Rabbit -
アプリケーション
適用あり: Sandwich ELISAmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab302751 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human ADAR1. The recommended pair for sandwich ELISA is:
Capture: ab302750, human ADAR1 Capture Antibody (unconjugated)
Detector: ab302751, human ADAR1 Detector Antibody (unconjugated)
The reference range value is 0.078 - 10 ng/mL.Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR24438-41 -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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sELISA pair antibody
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab302751の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR24438-72] to ADAR1 (ab302750).
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR24438-72] to ADAR1 (ab302750). |
ターゲット情報
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機能
Converts multiple adenosines to inosines and creates I/U mismatched base pairs in double-helical RNA substrates without apparent sequence specificity. Has been found to modify more frequently adenosines in AU-rich regions, probably due to the relative ease of melting A/U base pairs as compared to G/C pairs. Functions to modify viral RNA genomes and may be responsible for hypermutation of certain negative-stranded viruses. Edits the messenger RNAs for glutamate receptor (GLUR) subunits by site-selective adenosine deamination. Produces low-level editing at the GLUR-B Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Binds to short interfering RNAs (siRNA) without editing them and suppresses siRNA-mediated RNA interference. Binds to ILF3/NF90 and up-regulates ILF3-mediated gene expression. -
組織特異性
Ubiquitously expressed, highest levels were found in brain and lung. -
関連疾患
Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH) [MIM:127400]; also known as reticulate acropigmentation of Dohi. DSH is a pigmentary genodermatosis of autosomal dominant inheritance characterized by a mixture of hyperpigmented and hypopigmented macules distributed on the dorsal parts of the hands and feet. -
配列類似性
Contains 1 A to I editase domain.
Contains 2 DRADA repeats.
Contains 3 DRBM (double-stranded RNA-binding) domains. -
翻訳後修飾
Sumoylation reduces RNA-editing activity. -
細胞内局在
Cytoplasm. Nucleus > nucleolus. Isoform 1 is found predominantly in cytoplasm but appears to shuttle between the cytoplasm and nucleus. Isoform 5 is found exclusively in the nucleolus. - Information by UniProt
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参照データベース
- Entrez Gene: 103 Human
- Omim: 146920 Human
- SwissProt: P55265 Human
- Unigene: 12341 Human
- Unigene: 679967 Human
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別名
- 136 kDa double-stranded RNA-binding protein antibody
- 136kDa double stranded RNA binding protein antibody
- Adar 1 antibody
see all
画像
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Sandwich ELISA - Anti-ADAR1 antibody [EPR24438-41] - BSA and Azide free (Detector) (ab302751)Sandwich ELISA of ab302749 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
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![Anti-ADAR1 antibody [EPR24438-41] (BSA and Azide free (Detector)) (AB302751)](https://www.abcam.co.jp/ps/products/302/ab302751/Images/AB302751-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-ADAR1 antibody [EPR24438-41] (BSA and Azide free (Detector)) (AB302751)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab302751 は論文での使用が確認できていません。
