Anti-ADAMTS5 抗体 (ab41037)
Key features and details
- Rabbit polyclonal to ADAMTS5
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ADAMTS5 antibody
ADAMTS5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ADAMTS5 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human ADAMTS5 aa 600-700 conjugated to keyhole limpet haemocyanin.
(Peptide available asab41036) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab41037の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (3) |
1/250. Detects a band of approximately 73 kDa (predicted molecular weight: 102 kDa).
ab41037 appears to detect the 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase. |
| 特記事項 |
|---|
|
WB
1/250. Detects a band of approximately 73 kDa (predicted molecular weight: 102 kDa). ab41037 appears to detect the 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase. |
ターゲット情報
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機能
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period. -
組織特異性
Expressed at low level in placenta primarily but also detected in heart and brain, cervix, uterus, bladder, esophagus, rib cartilage, chondroblastoma, fibrous tissue and a joint capsule from an arthritic patient. -
配列類似性
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 2 TSP type-1 domains. -
ドメイン
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻訳後修飾
The precursor is cleaved by a furin endopeptidase. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 11096 Human
- Entrez Gene: 23794 Mouse
- Entrez Gene: 304135 Rat
- Omim: 605007 Human
- SwissProt: Q9UNA0 Human
- SwissProt: Q9R001 Mouse
- Unigene: 58324 Human
- Unigene: 112933 Mouse
see all -
別名
- A disintegrin and metalloproteinase with thrombospondin motifs 11 antibody
- A disintegrin and metalloproteinase with thrombospondin motifs 5 antibody
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5 antibody
see all
画像
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Western blot - Anti-ADAMTS5 antibody (ab41037)All lanes : Anti-ADAMTS5 antibody (ab41037) at 1/250 dilution
Lane 1 : NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 5 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 6 : Spinal Cord (Mouse) Tissue Lysate
Lane 7 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 8 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 9 : Brain (Rat) Tissue Lysate - normal tissue
Lane 10 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted?ab41037 appears to detect at 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase.
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Western blot - Anti-ADAMTS5 antibody (ab41037)All lanes : Anti-ADAMTS5 antibody (ab41037) at 1/250 dilution
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 :Mouse placenta normal tissue lysate - total protein (ab29139)
Lane 3 : Uterus (Human) Whole Cell Lysate - normal tissue
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted?
Additional bands at: 27 kDa. We are unsure as to the identity of these extra bands.ab41037 appears to detect a 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase. The doublet observed in human uterus lysate is possibly due to glycosylation.
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Western blot - Anti-ADAMTS5 antibody (ab41037)All lanes : Anti-ADAMTS5 antibody (ab41037) at 1 µg/ml (blocked with 3% Milk)
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutesab41037 appears to detect at 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (198)
ab41037 は 198 報の論文で使用されています。
- Jiang D et al. microRNA-105-5p protects against chondrocyte injury, extracellular matrix degradation, and osteoarthritis progression by targeting SPARCL1. Histol Histopathol 39:483-496 (2024). PubMed: 37534667
- Xu J et al. Tanshinone IIA alleviates chondrocyte apoptosis and extracellular matrix degeneration by inhibiting ferroptosis. Open Life Sci 18:20220666 (2023). PubMed: 37589005
- Uapinyoying P et al. Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle. iScience 26:107479 (2023). PubMed: 37599828
- Chen Z et al. Nucleus Pulposus-Targeting Nanocarriers Facilitate Mirna-Based Therapeutics for Intervertebral Disc Degeneration. Adv Healthc Mater 12:e2301337 (2023). PubMed: 37625164
- Huang J et al. Loss of miR-204 and miR-211 shifts osteochondral balance and causes temporomandibular joint osteoarthritis. J Cell Physiol 238:2668-2678 (2023). PubMed: 37697972