Anti-ADAMTS16 抗体 (ab45048)
Key features and details
- Rabbit polyclonal to ADAMTS16
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-ADAMTS16 antibody
ADAMTS16 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ADAMTS16 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide based on the propeptide region of human ADAMTS-16.
Immunogen の所有権に関して (Peptide available as ab45246.) -
特記事項
We have a range of domain specific antibodies for this target. For a full list please see all ADAMTS16 antibodies.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 2.9% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab45048の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
| 特記事項 |
|---|
追加情報
WB: 1/1000 - 1/5000. Predicted molecular weight: 136 kDa. The recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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関連性
ADAMTS16 is a member of the ADAMs family of proteinases with 4 thrombospondin-like domains. ADAMTS16 is closest in homology to ADAMTS18, sharing 57% overall identity. ADAMTS16 is expressed in fetal human lung and kidney, and in adult brain and ovary as well as in osteoarthritic cartilage, prostate and breast tissue. ADAMTS16 contains a prohormone-convertase cleavage site; the predicted PC cleavage site is the RRRR87 sequence in the 1224 and 1072 amino acid forms. This catalytic site is typical of the metalloproteinase catalytic domains, with an HExxHxxxxxH sequence. Several splice variants of ADAMTS16 have been reported. -
細胞内局在
Secreted -
参照データベース
- Entrez Gene: 271127 Mouse
- SwissProt: Q69Z28 Mouse
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別名
- A disintegrin and metalloproteinase with thrombospondin motifs 16 antibody
- ADAMTS 16 antibody
- ADAMTS16 antibody
- EC 3.4.24. antibody
画像
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Western blot - Anti-ADAMTS16 antibody (ab45048)All lanes : Anti-ADAMTS16 antibody (ab45048) at 1/1000 dilution
Lane 1 : Whole cell lysate prepared from mouse lung mesenchymal MLg cells (treated with SF)
Lane 2 : Whole cell lysate prepared from mouse lung mesenchymal MLg cells (treated with TPA)
Lane 3 : Whole cell lysate prepared from mouse lung mesenchymal MLg cells (treated with IL1-alpha)
Lane 4 : Whole cell lysate prepared from mouse lung mesenchymal MLg cells (treated with RA)
Predicted band size: 136 kDa
Several different splice variants of ADAMTS-16 have been reported: 151.38 kDa, 136.2 kDa and 119.5 kDa.Two shorter versions of 62kDa and 56kDa are also reported, lacking the MP domain.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab45048 は 5 報の論文で使用されています。
- Bai C & Chen DG ADAM metallopeptidase with thrombospondin type 1 motif 16 is a potential marker for prognosis in clear cell renal cell carcinoma. Pathol Int 73:297-305 (2023). PubMed: 37132990
- Yao Y et al. ADAMTS16 activates latent TGF-ß, accentuating fibrosis and dysfunction of the pressure-overloaded heart. Cardiovasc Res 116:956-969 (2020). PubMed: 31297506
- Mani S et al. Epigenetic changes in preterm birth placenta suggest a role for ADAMTS genes in spontaneous preterm birth. Hum Mol Genet 28:84-95 (2019). PubMed: 30239759
- Cao M et al. Metalloproteinase Adamts16 Is Required for Proper Closure of the Optic Fissure. Invest Ophthalmol Vis Sci 59:1167-1177 (2018). PubMed: 29625437
- Jacobi CL et al. Transcriptional regulation by the Wilms tumor protein, Wt1, suggests a role of the metalloproteinase Adamts16 in murine genitourinary development. J Biol Chem 288:18811-24 (2013). PubMed: 23661704