Anti-ADAMTS15 抗体 (ab45047)
Key features and details
- Rabbit polyclonal to ADAMTS15
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ADAMTS15 antibody -
製品の詳細
Rabbit polyclonal to ADAMTS15 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide based on the propeptide region of human ADAMTS-15 ADAMTS15.(Peptide available as ab45243.)
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特記事項
We have a range of domain specific antibodies for this target. For a full list please see all ADAMTS15 antibodies.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 2.9% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab45047の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
| 特記事項 |
|---|
追加情報
WB: 1/1000 - 1/5000. Predicted molecular weight: 103 kDa. The recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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組織特異性
Expressed in fetal liver and kidney, but not in any of the adult tissues examined. -
配列類似性
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 3 TSP type-1 domains. -
ドメイン
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻訳後修飾
The precursor is cleaved by a furin endopeptidase.
Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 170689 Human
- Omim: 607509 Human
- SwissProt: Q8TE58 Human
- Unigene: 534221 Human
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別名
- A disintegrin and metalloproteinase with thrombospondin motifs 15 antibody
- A disintegrin like and metalloprotease reprolysin type with thrombospondin type 1 motif 15 antibody
- A disintegrin like and metalloprotease reprolysin type with thrombospondin type 1 motif 15 preproprotein antibody
see all
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab45047 は 4 報の論文で使用されています。
- Addinsall AB et al. Treatment of Dystrophic mdx Mice with an ADAMTS-5 Specific Monoclonal Antibody Increases the Ex Vivo Strength of Isolated Fast Twitch Hindlimb Muscles. Biomolecules 10:N/A (2020). PubMed: 32156081
- McRae N et al. Glucocorticoids Improve Myogenic Differentiation In Vitro by Suppressing the Synthesis of Versican, a Transitional Matrix Protein Overexpressed in Dystrophic Skeletal Muscles. Int J Mol Sci 18:N/A (2017). PubMed: 29211034
- Dancevic CM et al. Biosynthesis and expression of a disintegrin-like and metalloproteinase domain with thrombospondin-1 repeats-15: a novel versican-cleaving proteoglycanase. J Biol Chem 288:37267-76 (2013). WB ; Human . PubMed: 24220035
- Molokwu CN et al. Androgen regulates ADAMTS15 gene expression in prostate cancer cells. Cancer Invest 28:698-710 (2010). WB ; Human . PubMed: 20590445