Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-ADAMTS13 抗体 (ab71550)

Reviews (1)Q&A (2)References (3)

Key features and details

  • Rabbit polyclonal to ADAMTS13
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-ADAMTS13 antibody [EPR6132] (ab177940)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-ADAMTS13 antibody
    ADAMTS13 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to ADAMTS13

  • 由来種

    Rabbit

  • 特異性

    This antibody reacts specifically with human 154 kDa ADAMTS13 protein

  • アプリケーション

    適用あり: IHC-P, WBmore details

  • 種交差性

    交差種: Mouse
    交差が予測される動物種: Human

  • 免疫原

    Synthetic peptide derived from the N-terminal domain of ADAMTS13 protein.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab71550の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration.
WB (1)
Use at an assay dependent concentration. Predicted molecular weight: 154 kDa.
特記事項
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 154 kDa.

ターゲット情報

  • 機能

    Cleaves the vWF multimers in plasma into smaller forms.

  • 組織特異性

    Plasma. Expressed primarily in liver.

  • 関連疾患

    Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP) [MIM:274150]; also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever.

  • 配列類似性

    Contains 2 CUB domains.
    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
    Contains 8 TSP type-1 domains.

  • ドメイン

    The pro-domain is not required for folding or secretion and does not perform the common function of maintening enzyme latency.
    The spacer domain is necessary to recognize and cleave vWF. The C-terminal TSP type-1 and CUB domains may modulate this interaction.

  • 翻訳後修飾

    May contain a C-mannosylation site and O-fucosylation sites in the TSP type-1 domains.
    The precursor is processed by a furin endopeptidase which cleaves off the pro-domain.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • A disintegrin and metalloproteinase with thrombospondin motifs 13 antibody
      • A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 13 antibody
      • A disintegrin like and metalloprotease with thrombospondin type 1 motif 13 antibody
      • ADAM metallopeptidase with thrombospondin type 1 motif 13 antibody
      • ADAM TS antibody
      • ADAM-TS 13 antibody
      • ADAM-TS13 antibody
      • ADAMTS 13 antibody
      • ADAMTS-13 antibody
      • ADAMTS13 antibody
      • ADAMTS13 protein antibody
      • ATS13_HUMAN antibody
      • C9orf8 antibody
      • TTP antibody
      • Von Willebrand factor cleaving protease antibody
      • von Willebrand factor-cleaving protease antibody
      • vWF cleaving protease antibody
      • vWF CP antibody
      • vWF-cleaving protease antibody
      • vWF-CP antibody
      • vWFCP antibody
      see all

    参考文献 (3)

    ab71550 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab71550 は 3 報の論文で使用されています。

    • Abu El-Asrar AM  et al. Differential Expression and Localization of ADAMTS Proteinases in Proliferative Diabetic Retinopathy. Molecules 27:N/A (2022). PubMed: 36144730
    • Bartoli CR  et al. Insights into the mechanism(s) of von Willebrand factor degradation during mechanical circulatory support. J Thorac Cardiovasc Surg N/A:N/A (2013). WB . PubMed: 24139617
    • Pos W  et al. An autoantibody epitope comprising residues R660, Y661, and Y665 in the ADAMTS13 spacer domain identifies a binding site for the A2 domain of VWF. Blood 115:1640-9 (2010). WB ; Human . PubMed: 20032502

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-ADAMTS13 antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (hepatocytes)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Sep 10 2015

    Question

    What's the concentration? Customer wamnts to test in ELISA, however, her WB results may indicate that the antibody isn't performing as it should in the first place.

    Read More
    Answer

    Laut unseren Unterlagen hatten Sie Probleme mit ab71550 Anti-ADAMTS13 antibody und haben unseren technischen Dienst deswegen kontaktiert.

    Ich sehe, dass wir noch auf einige Angaben hinsichtlich Ihrer Bestellung warten, die uns helfen werden Ihr Problem zu lösen. Falls Sie diese Informationen schon an uns geschickt haben, sieht es so aus, als ob diese nicht bei uns angekommen sind und ich möchte Sie daher bitten, diese Informationen nochmals an mich zu senden. Ich möchte Sie ausserdem für diesen Umstand um Entschuldigung bitten.

    Falls Sie das Problem selbst gelöst haben, möchte ich Sie bitten, mir dieses kurz mitzuteilen, damit ich Ihre Anfrage aus dem System löschen kann.

    Ich wünsche Ihnen viel Erfolg bei Ihrem Projekt und würde mich freuen von Ihnen zu hören, wie Sie vorankommen. Bitte zögern Sie nicht sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

    Read More

    Question

    I recieved an antibody today which I ordered. I am just wondering if you could tell me the concentration of the antibody in question-Ab71550?

    Kind regards,

    Read More
    Answer

    Thank you for your enquiry.

    I can confirm that ab71550 antibody is sold as whole antiserum. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and serum / ascites / tissue culture supernatant contains a lot of other proteins, which means the antibody quantification would not be accurate.

    I can confirm that for whole antiserum, concentration of antibody is known to vary between 1 - 10 mg/ml.

    I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com