Anti-ADAM23 抗体 (ab28304)
Key features and details
- Rabbit polyclonal to ADAM23
- Suitable for: ICC/IF
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-ADAM23 antibody
ADAM23 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ADAM23 -
由来種
Rabbit -
特異性
ab28304 recognises the Propeptide domain of ADAM23. -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Rat -
免疫原
Synthetic peptide corresponding to the Propeptide of Human ADAM23.
Immunogen の所有権に関して (Peptide available as ab41222.) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab28304の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
| 特記事項 |
|---|
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 for chemiluminescent substrates. Predicted molecular weight: 92 kDa. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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関連性
ADAM23 is a non-catalytic metalloprotease-like protein. It is highly expressed in the brain, primarily in the amygdala, caudate nucleus, hypothalamus, thalamus, cerebral cortex and occipital pole, and weakly expressed in the heart. 3 isoforms are produced by alternative splicing, alpha, beta and gamma. ADAM23 may play a role in cell-cell and cell-matrix interactions. -
細胞内局在
Single pass type I membrane protein. 3 isoforms produced by alternative splicing, gamma isoform is secreted. -
参照データベース
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別名
- A disintegrin and metalloproteinase domain 23 antibody
- ADAM metallopeptidase domain 23 antibody
- Disintegrin and metalloproteinase domain containing protein 23 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-ADAM23 antibody (ab28304)ICC/IF image of ab28304 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28304, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab28304 は 3 報の論文で使用されています。
- Fels E et al. Dysregulation of the hippocampal neuronal network by LGI1 auto-antibodies. PLoS One 17:e0272277 (2022). PubMed: 35984846
- Endmayr V et al. Anti-Neuronal IgG4 Autoimmune Diseases and IgG4-Related Diseases May Not Be Part of the Same Spectrum: A Comparative Study. Front Immunol 12:785247 (2021). PubMed: 35095860
- McCracken L et al. Improving the antibody-based evaluation of autoimmune encephalitis. Neurol Neuroimmunol Neuroinflamm 4:e404 (2017). PubMed: 29075658