AB284849

Anti-ADAM2 抗体 [EP15281] - BSA and Azide free

Anti-ADAM2 antibody [EP15281] - BSA and Azide free

RabMAb
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Rabbit Monoclonal ADAM2 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

別名を表示する

FTNB, ADAM2, Disintegrin and metalloproteinase domain-containing protein 2, ADAM 2, Cancer/testis antigen 15, Fertilin subunit beta, PH-30, PH30-beta, CT15, PH30

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM2 antibody [EP15281] - BSA and Azide free (AB284849)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM2 antibody [EP15281] - BSA and Azide free (AB284849)

This data was developed using ab193668, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling ADAM2 with ab193668 at 1/1000 dilution (1μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-ADAM2 antibody [EP15281] - BSA and Azide free (AB284849)

This data was developed using ab193668, the same antibody clone in a different buffer formulation.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ADAM2 antibody [EP15281] (<a href='/products/primary-antibodies/adam2-antibody-ep15281-ab193668'>ab193668</a>) at 1/1000 dilution

All lanes:

Human testis lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 82 kDa

Observed band size: 100 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP15281

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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製品の詳細

ab284849 is the carrier-free version of ab193668

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ADAM2 also known as fertilin beta is a member of the ADAM (a disintegrin and metalloprotease) family. It is a protein with a mass of approximately 100 kDa. ADAM2 contains a disintegrin and metalloproteinase domain which are responsible for its adhesive and protease activities. Expression of ADAM2 occurs mainly in the testis especially in mature spermatozoa where it plays essential roles in reproduction processes.

Biological function summary

The ADAM2 protein functions in sperm-egg interactions facilitating sperm binding to the egg's surface during fertilization. It is part of a multi-protein complex that also includes ADAM1 which enhances the binding efficiency to the egg zona pellucida. ADAM2's disintegrin domain is thought to interact with integrins on the egg membrane while the metalloproteinase domain might process surrounding proteins to prepare for fertilization.

Pathways

ADAM2 actively participates in reproduction-related signaling pathways particularly the integrin-mediated cell adhesion pathway. It collaborates with other ADAM family proteins and integrins to ensure successful fertilization. Another important pathway is the gamete fusion pathway where ADAM2 assists in the fusion process alongside other proteins such as ADAM3 ensuring gamete membrane fusion and subsequent fertilization.

Abnormalities in ADAM2 expression or function can lead to male infertility making this protein a biomarker of reproductive health concerns. It has a known association with other ADAM proteins such as ADAM3 which also plays a role in sperm maturation and fertility. Researchers are interested in how alterations in ADAM2 and these related proteins contribute to infertility offering potential insights for therapeutic interventions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. Could have a direct role in sperm-zona binding or migration of sperm from the uterus into the oviduct. Interactions with egg membrane could be mediated via binding between its disintegrin-like domain to one or more integrins receptors on the egg. This is a non catalytic metalloprotease-like protein.

See full target information ADAM2

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ADAM2 抗体 [EP15281] - BSA and Azide free

Anti-ADAM2 antibody [EP15281] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM2 antibody [EP15281] - BSA and Azide free (AB284849)

Western blot - Anti-ADAM2 antibody [EP15281] - BSA and Azide free (AB284849)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

組合せ製品

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Secondary Antibodies

Abcam product promise