Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-ADAM17 抗体 [EPR21129-131] - BSA and Azide free (ab291100)

Submit a review Submit a question
Western blot - Anti-ADAM17 antibody [EPR21129-131] - BSA and Azide free (ab291100)
  • Western blot - Anti-ADAM17 antibody [EPR21129-131] - BSA and Azide free (ab291100)
  • Anti-ADAM17 antibody [EPR21129-131] - BSA and Azide free (ab291100)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21129-131] to ADAM17 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-ADAM17 antibody [EPR21129-131] - BSA and Azide free
    ADAM17 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR21129-131] to ADAM17 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Wild-type HCT116 whole cell lysate, HeLa whole cell lysate, HEK-293 whole cell lysate, THP-1 whole cell fresh lysate, Jurkat whole cell fresh lysate.

  • 特記事項

    ab291100 is a carrier free version of ab291073. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab291100の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 135, 80 kDa (predicted molecular weight: 93 kDa).
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 135, 80 kDa (predicted molecular weight: 93 kDa).
追加情報
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

ターゲット情報

  • 機能

    Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.

  • 組織特異性

    Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.

  • 配列類似性

    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.

  • ドメイン

    Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

  • 翻訳後修飾

    The precursor is cleaved by a furin endopeptidase.
    Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
      • A disintegrin and metalloproteinase domain 17 antibody
      • ADA17_HUMAN antibody
      • ADAM 17 antibody
      • ADAM metallopeptidase domain 17 antibody
      • ADAM17 antibody
      • ADAM17 protein antibody
      • CD 156b antibody
      • CD156b antibody
      • CD156b antigen antibody
      • CSVP antibody
      • Disintegrin and metalloproteinase domain-containing protein 17 antibody
      • MGC71942 antibody
      • NISBD antibody
      • NISBD1 antibody
      • Snake venom like protease antibody
      • Snake venom-like protease antibody
      • TACE antibody
      • TNF alpha convertase antibody
      • TNF alpha converting enzyme antibody
      • TNF-alpha convertase antibody
      • TNF-alpha-converting enzyme antibody
      • Tumor Necrosis Factor Alpha Converting Enzyme antibody
      see all

    画像

    • Western blot - Anti-ADAM17 antibody [EPR21129-131] - BSA and Azide free (ab291100)
      Western blot - Anti-ADAM17 antibody [EPR21129-131] - BSA and Azide free (ab291100)
      All lanes : Anti-ADAM17 antibody [EPR21129-131] (ab291073) at 1/1000 dilution

      Lane 1 : Wild-type HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate
      Lane 2 : ADAM17 knockout HCT116 whole cell lysate
      Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 4 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 93 kDa
      Observed band size: 135, 80 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      This data was developed using ab291073, the same antibody clone in a different buffer formulation. 

      Blocking and dilution buffer and concentration: 5% NFDM/TBST 

      80 kDa mature ADAM17 and 135 kDa proform ADAM17 are observed.

      The expression profile is consistent with that described in the literature (PMID: 27731361).

      In Western blot, ab291073 was shown to bind specifically to ADAM17. Two bands were observed at 135/80 kDa in wild-type HCT116 cell lysates with no signal observed at this size in ADAM17 knockout cell lysates.

      This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

      Performed under reducing conditions. 

    • Western blot - Anti-ADAM17 antibody [EPR21129-131] - BSA and Azide free (ab291100)
      Western blot - Anti-ADAM17 antibody [EPR21129-131] - BSA and Azide free (ab291100)
      All lanes : Anti-ADAM17 antibody [EPR21129-131] (ab291073) at 1/1000 dilution

      Lane 1 : THP-1 (human monocytic leukemia monocyte) whole cell fresh lysate
      Lane 2 : Jurkat (human T cell leukemia T lymphocyte) whole cell fresh lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 93 kDa
      Observed band size: 135, 80 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      This data was developed using ab291073, the same antibody clone in a different buffer formulation. 

      Blocking and dilution buffer and concentration: 5% NFDM/TBST

      Lysates were prepared from fresh material and used for Western blotting immediately to minimize protein degradation.

      This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    • Anti-ADAM17 antibody [EPR21129-131] - BSA and Azide free (ab291100)
      Anti-ADAM17 antibody [EPR21129-131] - BSA and Azide free (ab291100)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab291100 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab291100 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab291100.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com