Anti-ADAM17 抗体 (ab39162)
Key features and details
- Rabbit polyclonal to ADAM17
- Suitable for: IP, ICC/IF, WB, IHC-P
- Reacts with: Rat, Human, Pig
- Isotype: IgG
製品の概要
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製品名
Anti-ADAM17 antibody
ADAM17 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ADAM17 -
由来種
Rabbit -
特異性
This antibody recognizes ADAM17, but does not react with other ADAMs. -
アプリケーション
適用あり: IP, ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Rat, Human, Pig -
免疫原
Synthetic peptide corresponding to Human ADAM17. Within the cytoplasmic domain of Human ADAM17.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Preservative: 0.01% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab39162の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP | (1) |
Use at an assay dependent concentration.
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| ICC/IF | (2) |
Use at an assay dependent concentration.
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| WB | (5) |
Use at an assay dependent concentration. Predicted molecular weight: 93 kDa.
1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. Detects a band of approximately 110 kDa in reduced Western blots of conditioned media or cell lysates. (Predicted molecular weight: 93 kDa). Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. |
| IHC-P | (1) |
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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IP
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 93 kDa. 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. Detects a band of approximately 110 kDa in reduced Western blots of conditioned media or cell lysates. (Predicted molecular weight: 93 kDa). Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. |
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IHC-P
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway. -
組織特異性
Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney. -
配列類似性
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain. -
ドメイン
Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻訳後修飾
The precursor is cleaved by a furin endopeptidase.
Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 6868 Human
- Entrez Gene: 57027 Rat
- Omim: 603639 Human
- SwissProt: P78536 Human
- SwissProt: Q9Z1K9 Rat
- Unigene: 404914 Human
- Unigene: 144585 Rat
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別名
- A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
- A disintegrin and metalloproteinase domain 17 antibody
- ADA17_HUMAN antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM17 antibody (ab39162)Ab39162 staining human normal colon. Staining is localised to membrane.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (43)
ab39162 は 43 報の論文で使用されています。
- Badenes M et al. The ADAM17 sheddase complex regulator iTAP/Frmd8 modulates inflammation and tumor growth. Life Sci Alliance 6:N/A (2023). PubMed: 36720499
- Shoykhet M et al. Cardiomyocyte cohesion is increased after ADAM17 inhibition. Front Cell Dev Biol 11:1021595 (2023). PubMed: 36733457
- Kahveci-Türköz S et al. A structural model of the iRhom-ADAM17 sheddase complex reveals functional insights into its trafficking and activity. Cell Mol Life Sci 80:135 (2023). PubMed: 37119365
- Yang JT et al. ADAM17 Confers Temozolomide Resistance in Human Glioblastoma Cells and miR-145 Regulates Its Expression. Int J Mol Sci 24:N/A (2023). PubMed: 37175410
- Darka Aslan I et al. Investigation of CD56, ADAM17 and FGF21 Expressions in the Placentas of Preeclampsia Cases. Medicina (Kaunas) 59:N/A (2023). PubMed: 37374349