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AB39162

Anti-ADAM17 抗体

Anti-ADAM17 antibody

4

(10 Reviews)

|

(54 Publications)

Anti-ADAM17 antibody (ab39162) is a rabbit polyclonal antibody detecting ADAM17 in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Pig, Rat.

- Over 40 publications
- Trusted since 2006

別名を表示する

CD156b, CSVP, TACE, ADAM17, Disintegrin and metalloproteinase domain-containing protein 17, ADAM 17, Snake venom-like protease, TNF-alpha convertase, TNF-alpha-converting enzyme

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM17 antibody (AB39162)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM17 antibody (AB39162)

ab39162 staining human normal colon. Staining is localised to membrane.
Left panel : with primary antibody at 4 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human, Rat, Pig

アプリケーション

ICC/IF, WB, IP, IHC-P

applications

免疫原

Synthetic Peptide within Human ADAM17. The exact immunogen used to generate this antibody is proprietary information.

P78536

特異性

This antibody recognizes ADAM17, but does not react with other ADAMs.

Reactivity data

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製品の詳細

What is this antibody validated in?
Anti-ADAM17 antibody (ab39162) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Pig, Rat samples.

What is the molecular weight of ADAM17?
Anti-ADAM17 (ab39162) specifically detects a band for ADAM17 (UniProt: P78536) at a molecular weight of 93kDa.

Trusted by the scientific community
Anti-ADAM17 (ab39162) was first used in a scientific publication in 2006 and has been cited over 40 times in peer-reviewed journals.

Reviewed by scientists
Anti-ADAM17 (ab39162) has over 5 independent reviews from customers.

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
Preservative: 0.01% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ADAM17 also known as TACE (tumor necrosis factor-alpha converting enzyme) is a metalloprotease belonging to the ADAM (a disintegrin and metalloprotease) family. This protein functions by cleaving and shedding extracellular portions of cell surface proteins. It weighs approximately 93 kDa. ADAM17 is expressed in a range of tissues including the heart liver kidney and brain. The protein plays a role in the regulation of various biological processes through the activation and inactivation of membrane-bound precursor proteins.
Biological function summary

The ADAM17 protein regulates a multitude of cellular responses such as inflammation and growth factor signaling. It is not part of a complex but interacts with various substrates in the cell membrane. ADAM17 cleaves precursors to release soluble cytokines and growth factors like tumor necrosis factor-alpha (TNF-alpha) and epidermal growth factor receptor (EGFR) ligands which modulate cell proliferation migration and apoptosis.

Pathways

ADAM17 plays an integral role in both the TNF and EGFR signaling pathways. These pathways are important in maintaining normal cellular homeostasis. ADAM17 interacts with proteins like TNF receptors and ligands of the EGFR family integrating signaling that affects immune response and cell growth. The regulatory function of ADAM17 in these pathways makes it an important target for modulating cellular responses triggered by external stimuli.

ADAM17 has significant implications in conditions such as rheumatoid arthritis and cancer. In rheumatoid arthritis the protein's shedding activity influences inflammatory cytokines contributing to the disease’s pathogenesis alongside proteins like TNF-alpha. In cancer ADAM17's ability to release EGFR ligands affects tumor cell proliferation and metastasis with interactions involving EGFR proteins. It is a target of therapeutic interest with research focused on developing inhibitors to manage these diseases effectively.

製品プロトコール

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ターゲットの情報

Transmembrane metalloprotease which mediates the ectodomain shedding of a myriad of transmembrane proteins including adhesion proteins, growth factor precursors and cytokines important for inflammation and immunity (PubMed : 24226769, PubMed : 24227843, PubMed : 28060820, PubMed : 28923481). Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form (PubMed : 36078095, PubMed : 9034191). Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface (PubMed : 20592283). Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein (PubMed : 12441351). Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT) (PubMed : 24226769). Plays a role in the proteolytic processing of ACE2 (PubMed : 24227843). Plays a role in hemostasis through shedding of GP1BA, the platelet glycoprotein Ib alpha chain (By similarity). Mediates the proteolytic cleavage of LAG3, leading to release the secreted form of LAG3 (By similarity). Mediates the proteolytic cleavage of IL6R, leading to the release of secreted form of IL6R (PubMed : 26876177, PubMed : 28060820). Mediates the proteolytic cleavage and shedding of FCGR3A upon NK cell stimulation, a mechanism that allows for increased NK cell motility and detachment from opsonized target cells. Cleaves TREM2, resulting in shedding of the TREM2 ectodomain (PubMed : 28923481).
See full target information ADAM17

文献 (54)

Recent publications for all applications. Explore the full list and refine your search

International journal of oral science 17:18 PubMed40075093

2025

Administration of Porphyromonas gingivalis in pregnant mice enhances glycolysis and histone lactylation/ADAM17 leading to cleft palate in offspring.

Applications

Unspecified application

Species

Unspecified reactive species

Xige Zhao,Xiaoyu Zheng,Yijia Wang,Jing Chen,Xiaotong Wang,Xia Peng,Dong Yuan,Ying Liu,Zhiwei Wang,Juan Du

Viruses 16: PubMed39459898

2024

Modulation of ADAM17 Levels by Pestiviruses Is Species-Specific.

Applications

Unspecified application

Species

Unspecified reactive species

Hann-Wei Chen,Marianne Zaruba,Aroosa Dawood,Stefan Düsterhöft,Benjamin Lamp,Till Ruemenapf,Christiane Riedel

Proteomics 24:e202400076 PubMed39318062

2024

Development of a Proteomic Workflow for the Identification of Heparan Sulphate Proteoglycan-Binding Substrates of ADAM17.

Applications

Unspecified application

Species

Unspecified reactive species

Matteo Calligaris,Donatella Pia Spanò,Maria Chiara Puccio,Stephan A Müller,Simone Bonelli,Margot Lo Pinto,Giovanni Zito,Carl P Blobel,Stefan F Lichtenthaler,Linda Troeberg,Simone Dario Scilabra

Scientific reports 14:14535 PubMed38914585

2024

Assessment of cerebrovascular alterations induced by inflammatory response and oxidative-nitrative stress after traumatic intracranial hypertension and a potential mitigation strategy.

Applications

Unspecified application

Species

Unspecified reactive species

Shangming Zhang,Yehuang Chen,Qizuan Chen,Hongjie Chen,Liangfeng Wei,Shousen Wang

Molecular cell 84:2152-2165.e5 PubMed38781971

2024

Cryo-EM reveals that iRhom2 restrains ADAM17 protease activity to control the release of growth factor and inflammatory signals.

Applications

Unspecified application

Species

Unspecified reactive species

Fangfang Lu,Hongtu Zhao,Yaxin Dai,Yingdi Wang,Chia-Hsueh Lee,Matthew Freeman

Cellular and molecular life sciences : CMLS 81:163 PubMed38570362

2024

iRhom2 regulates ectodomain shedding and surface expression of the major histocompatibility complex (MHC) class I.

Applications

Unspecified application

Species

Unspecified reactive species

Matteo Calligaris,Donatella P Spanò,Simone Bonelli,Stephan A Müller,Claudia Carcione,Danilo D'apolito,Giandomenico Amico,Monica Miele,Mariangela Di Bella,Giovanni Zito,Elisa Nuti,Armando Rossello,Carl P Blobel,Stefan F Lichtenthaler,Simone D Scilabra

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed38418221

2024

Novel Social Stimulation Ameliorates Memory Deficit in Alzheimer's Disease Model through Activating α-Secretase.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaoyun Ren,Susu Wang,Junru Li,Kun Cao,Mei Zhuang,Miao Wu,Junhua Geng,Zhengping Jia,Wei Xie,An Liu

Cellular and molecular life sciences : CMLS 81:102 PubMed38409522

2024

Pathological mutations reveal the key role of the cytosolic iRhom2 N-terminus for phosphorylation-independent 14-3-3 interaction and ADAM17 binding, stability, and activity.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Bläsius,Lena Ludwig,Sarah Knapp,Charlotte Flaßhove,Friederike Sonnabend,Diandra Keller,Nikola Tacken,Xintong Gao,Selcan Kahveci-Türköz,Caroline Grannemann,Aaron Babendreyer,Colin Adrain,Sebastian Huth,Jens Malte Baron,Andreas Ludwig,Stefan Düsterhöft

Molecular cell 84:277-292.e9 PubMed38183983

2024

Cleavage of the pseudoprotease iRhom2 by the signal peptidase complex reveals an ER-to-nucleus signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Iqbal Dulloo,Michael Tellier,Clémence Levet,Anissa Chikh,Boyan Zhang,Diana C Blaydon,Catherine M Webb,David P Kelsell,Matthew Freeman

American journal of physiology. Heart and circulatory physiology 326:H270-H277 PubMed37999645

2023

Neuraminidase-induced externalization of phosphatidylserine activates ADAM17 and impairs insulin signaling in endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Larissa Ferreira-Santos,Francisco I Ramirez-Perez,Christopher A Foote,Marc A Augenreich,Neil J McMillan,Morgan B Williams,Juan D Gonzalez-Vallejo,Gavin Power,Andrew A Wheeler,Camila Manrique-Acevedo,Luis A Martinez-Lemus,Jaume Padilla
View all publications

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