Anti-ADAM17 抗体
Anti-ADAM17 antibody
4
(10 Reviews)
|
(54 Publications)
Anti-ADAM17 antibody (ab39162) is a rabbit polyclonal antibody detecting ADAM17 in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Pig, Rat.
- Over 40 publications
- Trusted since 2006
別名を表示する
CD156b, CSVP, TACE, ADAM17, Disintegrin and metalloproteinase domain-containing protein 17, ADAM 17, Snake venom-like protease, TNF-alpha convertase, TNF-alpha-converting enzyme
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM17 antibody (AB39162)
ab39162 staining human normal colon. Staining is localised to membrane.
Left panel : with primary antibody at 4 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Reactivity data
製品の詳細
Anti-ADAM17 antibody (ab39162) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Pig, Rat samples.
What is the molecular weight of ADAM17?
Anti-ADAM17 (ab39162) specifically detects a band for ADAM17 (UniProt: P78536) at a molecular weight of 93kDa.
Trusted by the scientific community
Anti-ADAM17 (ab39162) was first used in a scientific publication in 2006 and has been cited over 40 times in peer-reviewed journals.
Reviewed by scientists
Anti-ADAM17 (ab39162) has over 5 independent reviews from customers.
出荷温度及び保存条件
製品の状態
精製方法
バッファー組成
出荷温度
短期保存期間
短期保存温度
長期保存温度
分注に関する情報
補足情報
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The ADAM17 protein regulates a multitude of cellular responses such as inflammation and growth factor signaling. It is not part of a complex but interacts with various substrates in the cell membrane. ADAM17 cleaves precursors to release soluble cytokines and growth factors like tumor necrosis factor-alpha (TNF-alpha) and epidermal growth factor receptor (EGFR) ligands which modulate cell proliferation migration and apoptosis.
Pathways
ADAM17 plays an integral role in both the TNF and EGFR signaling pathways. These pathways are important in maintaining normal cellular homeostasis. ADAM17 interacts with proteins like TNF receptors and ligands of the EGFR family integrating signaling that affects immune response and cell growth. The regulatory function of ADAM17 in these pathways makes it an important target for modulating cellular responses triggered by external stimuli.
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ターゲットの情報
文献 (54)
Recent publications for all applications. Explore the full list and refine your search
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Cellular and molecular life sciences : CMLS 81:163 PubMed38570362
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The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed38418221
2024
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Cellular and molecular life sciences : CMLS 81:102 PubMed38409522
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Molecular cell 84:277-292.e9 PubMed38183983
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American journal of physiology. Heart and circulatory physiology 326:H270-H277 PubMed37999645
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