Anti-ADAM17 抗体 (ab39161)
Key features and details
- Rabbit polyclonal to ADAM17
- Suitable for: ELISA
- Reacts with: Rat, Human, Pig
- Isotype: IgG
製品の概要
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製品名
Anti-ADAM17 antibody
ADAM17 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ADAM17 -
由来種
Rabbit -
特異性
This antibody recognizes ADAM17, but does not react with other ADAMs. -
アプリケーション
適用あり: ELISAmore details -
種交差性
交差種: Rat, Human, Pig -
免疫原
Synthetic peptide based on the propeptide domain of human ADAM17.
Immunogen の所有権に関して (Peptide available as ab41213.) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab39161の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
ELISA
Use at an assay dependent concentration. |
ターゲット情報
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機能
Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway. -
組織特異性
Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney. -
配列類似性
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain. -
ドメイン
Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻訳後修飾
The precursor is cleaved by a furin endopeptidase.
Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 6868 Human
- Entrez Gene: 57027 Rat
- Omim: 603639 Human
- SwissProt: P78536 Human
- SwissProt: Q9Z1K9 Rat
- Unigene: 404914 Human
- Unigene: 144585 Rat
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別名
- A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
- A disintegrin and metalloproteinase domain 17 antibody
- ADA17_HUMAN antibody
see all
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab39161 は 5 報の論文で使用されています。
- Zhang Y & Fang D The incorporation loci of H3.3K36M determine its preferential prevalence in chondroblastomas. Cell Death Dis 12:311 (2021). PubMed: 33762579
- Grieve AG et al. Phosphorylation of iRhom2 at the plasma membrane controls mammalian TACE-dependent inflammatory and growth factor signalling. Elife 6:N/A (2017). PubMed: 28432785
- Siggs OM et al. Genetic interaction implicates iRhom2 in the regulation of EGF receptor signalling in mice. Biol Open 3:1151-7 (2014). PubMed: 25395669
- Brooke MA et al. iRHOM2-dependent regulation of ADAM17 in cutaneous disease and epidermal barrier function. Hum Mol Genet N/A:N/A (2014). Human . PubMed: 24643277
- Chavaroche A et al. Glycosylation of a disintegrin and metalloprotease 17 affects its activity and inhibition. Anal Biochem 449:68-75 (2014). PubMed: 24361716