Anti-ADAM12 抗体 - Aminoterminal end (ab39155)

On our datasheet for ab39155 we suggest using human fetal fibroblasts as a positive control. Otherwise other positive controls which can be used are: Hela cells, A431 cells or human chondrosarcoma.

Vielen Dank für Ihre Email und für Ihr Interesse an unseren Produkten.
Unserem Wissen nach wurde ab39155 bisher noch nicht in IHC-Fr getestet und auch nicht in dieser Anwendung publiziert.
Falls Sie dies selbst testen möchten, kann ich Ihnen zur Zeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von ab39155 abdecken, und gegen eine erneute Bestellung eines weiteren Produkts von uns verrechnet werden.
Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:
1.) Bestätigen Sie mir bitte, dass Sie ab39155 in IHC-Fr testen möchten.
2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.
3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax
4.) Testen Sie den Antikörper in IHC-Fr.
5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes"
Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
https://www.abcam.com/abreviews
6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können ein anderes Produkt zu dem gleichen Preis wie ab39155 bei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode bereit). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.
Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.
Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
https://www.abcam.com/collaborationdiscount
Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number "1131954" ab56366.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

No problem, I will send the product ab56366.

Could you let me know the purchase order number of ab39155?

Thanks!

Thank you for your email.

Lab has confirmed that the immunogen for ab115203 and ab28747 is indeed corresponds to N-terminal end and is in signal peptide region, so these antibodies might not be suitable.

Regarding ab56366 this antibody is tested only with recombinant protein used as immunogen; this hasn't yet been tested with endogenous lysates or cell. However If you are interested in using this antibody, then I can offer you Abreview discount along with this replacement. Please click the following link for more information about 100% Abreview discount.

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

Thirdly, I can offer you a vial of ab39155 from different lot. It may well be that the problem you are experiencing due to damaged vial etc.

Could you please let me know how you would like to proceed?

I have requested confirmation of immunogen sequence from lab. I will get back to you with the reply soon. Once it gets confirmed I will then be able to suggest the suitable antibody.

Many thanks for having patience.

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

Thank you for taking the time to provide us with the further details. I apologize for the delay of my reply. I am very sorry, that you are experiencing problems with two of our antibodies. I do actually suspect that you have no signal of Adam12 and the bands at 50kD are unspecific. Indeed, I am not aware of any isoform of adam12 around that molecular weight. On Swissprot, there are several isoformes described, all however above 50kD. http://www.uniprot.org/uniprot/O43184 I am therefore sorry not being able to help you to îdentify this band. I can recommend however to add protease inhibitors to the samples in order to exclude that these bands might be degradation products. I have looked throught the details provided, which will also enable us to investigate this case and will provide us with vital information for monitoring product quality. Having reviewed this case, I would like to offer some suggestions to help optimize the results from these two antibodies. I would also appreciate if you can confirm some further details. In the event that we can not solve the problems with these suggestions (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund. 1.) I have noticed that the blocking has been performed in a 0.05% Milk solution. I suppose this has been done for one hour. I can strongly recommend to test also another blocking solution, such as 5% BSA. Also, I do normally recommend to use also a 5% milk solution. Indeed, changing the blocking solution can drastically influence and improve the results. Please see the image on the datasheet of ab9385 as an example. Click here (or use the following: https://www.abcam.com/HRP-GAPDH-antibody-Loading-Control-ab9385.html). 2.) I can also suggest to use the primary antibodies in concentration of 1/1000. 3.) As mentinned above, I can recommend to include protease inhibitors in the samples if this has not yet been done sone. If the ab29431 has been used, can you please confirm that the lysat has been stored correctly? Thank you! 4,) I would also apprecaite if you can confirm the quality of the secondary antibody: Does the secondary antibody alone not give such a similar background? Does the secondary antibody work well with other primary antibodies? I hope this information is helpful, and I thank you for your cooperation. I am looking forward to hear back from you with your comments and/or results.