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Anti-ADAM12 抗体 (ab115203)

Submit a review Q&A (4)References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM12 antibody (ab115203)
  • Western blot - Anti-ADAM12 antibody (ab115203)

Key features and details

  • Goat polyclonal to ADAM12
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-ADAM12 antibody
    ADAM12 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to ADAM12

  • 由来種

    Goat

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide:

    AARPLPVSPARAL

    with a C terminal Cysteine residue linker, corresponding to N terminal amino acids 2-14 of Human ADAM12 (NP_003465.3).
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • Human placenta tissue; Human heart lysate.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab115203の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 100 kDa.
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 100 kDa.
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.

  • 組織特異性

    Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.

  • 配列類似性

    Contains 1 disintegrin domain.
    Contains 1 EGF-like domain.
    Contains 1 peptidase M12B domain.

  • ドメイン

    The cysteine-rich domain supports cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. In carcinomas cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

  • 翻訳後修飾

    The precursor is cleaved by a furin endopeptidase.

  • 細胞内局在

    Secreted and Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • A disintegrin and metalloproteinase domain 12 antibody
      • ADA12_HUMAN antibody
      • ADAM 12 antibody
      • ADAM metallopeptidase domain 12 antibody
      • ADAM12 antibody
      • CAR10 antibody
      • Disintegrin and metalloproteinase domain-containing protein 12 antibody
      • MCMP antibody
      • MCMPMltna antibody
      • Meltrin alpha antibody
      • Meltrin-alpha antibody
      • Metalloprotease disintegrin 12 transmembrane antibody
      • MLTN antibody
      • MLTNA antibody
      • OTTHUMP00000046766 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM12 antibody (ab115203)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM12 antibody (ab115203)
      ab115203 at 3.75 µg/ml staining ADAM12 in Formalin-fixed, Paraffin-embedded Human placenta tissue by Immunohistochemistry.
    • Western blot - Anti-ADAM12 antibody (ab115203)
      Western blot - Anti-ADAM12 antibody (ab115203)
      Anti-ADAM12 antibody (ab115203) at 0.1 µg/ml + Human heart lysate (in RIPA buffer) at 30 µg

      Developed using the ECL technique.

      Predicted band size: 100 kDa

    参考文献 (1)

    ab115203 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab115203 は 1 報の論文で使用されています。

    • Jana S  et al. Disparate Remodeling of the Extracellular Matrix and Proteoglycans in Failing Pediatric Versus Adult Hearts. J Am Heart Assoc 7:e010427 (2018). PubMed: 30371322

    レビューと Q&A

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    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Question

    The original PO number was LLZS964855. Let me know if you need anything else.

    Thanks,

    Read More
    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number "1131954" ab56366.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Question

    Would it be possible to try a vial of ab56366 please? I know it hasn’t been tried on endogenous protein but its immunological sequence looks the most hopeful for using with Flow cytometry.
    Do you need me to send you my delivery address?
    Thanks,

    Read More
    Answer

    No problem, I will send the product ab56366.

    Could you let me know the purchase order number of ab39155?

    Thanks!

    Read More

    Question

    Thank you for your reply.

    I would ideally like a replacement antibody as I still need to validate the ADAM12 result for publication. Would you be able to advise whether you think any of the alternative ADAM12 antibodies would be suitable for a flow cytometry application. Looking at the data sheets, although none of your alternative antibodies are tested in flow cytometry, both ab115203 and ab28747 are supposed to recognise a N-terminal fragment of the protein and give a band by western blotting of the correct size. However I think they both possibly recognise a region within the signal peptide sequence of ADAM12 rather than the rest of the protein – is this corrent. AB56366 is also supposed to recognise N-terminal amino acids 208-305, which I believe should also be extracellular, however it has only been used of recombinant fragments of hADAM12, rather than endogenous protein. Do you have any other data on these antibodies to suggest that they may be useful for flow cytometry?

    Thanks,

    Read More
    Answer

    Thank you for your email.

    Lab has confirmed that the immunogen for ab115203 and ab28747 is indeed corresponds to N-terminal end and is in signal peptide region, so these antibodies might not be suitable.

    Regarding ab56366 this antibody is tested only with recombinant protein used as immunogen; this hasn't yet been tested with endogenous lysates or cell. However If you are interested in using this antibody, then I can offer you Abreview discount along with this replacement. Please click the following link for more information about 100% Abreview discount.

    https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

    Thirdly, I can offer you a vial of ab39155 from different lot. It may well be that the problem you are experiencing due to damaged vial etc.

    Could you please let me know how you would like to proceed?

    Read More

    Question

    Thank you for your reply.

    I would ideally like a replacement antibody as I still need to validate the ADAM12 result for publication. Would you be able to advise whether you think any of the alternative ADAM12 antibodies would be suitable for a flow cytometry application. Looking at the data sheets, although none of your alternative antibodies are tested in flow cytometry, both ab115203 and ab28747 are supposed to recognise a N-terminal fragment of the protein and give a band by western blotting of the correct size. However I think they both possibly recognise a region within the signal peptide sequence of ADAM12 rather than the rest of the protein – is this corrent. AB56366 is also supposed to recognise N-terminal amino acids 208-305, which I believe should also be extracellular, however it has only been used of recombinant fragments of hADAM12, rather than endogenous protein. Do you have any other data on these antibodies to suggest that they may be useful for flow cytometry?

    Thanks,

    Read More
    Answer

    I have requested confirmation of immunogen sequence from lab. I will get back to you with the reply soon. Once it gets confirmed I will then be able to suggest the suitable antibody.

    Many thanks for having patience.

    Read More

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