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AB276047

Anti-ADAM12 抗体 [737] - BSA and Azide free

Anti-ADAM12 antibody [737] - BSA and Azide free

4

(1 Review)

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(1 Publication)

Rabbit Recombinant Monoclonal ADAM12 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ADAM12 aa 1-550.

別名を表示する

MLTN, UNQ346/PRO545, ADAM12, Disintegrin and metalloproteinase domain-containing protein 12, ADAM 12, Meltrin-alpha

2 Images
Immunoprecipitation - Anti-ADAM12 antibody [737] - BSA and Azide free (AB276047)
  • IP

Unknown

Immunoprecipitation - Anti-ADAM12 antibody [737] - BSA and Azide free (AB276047)

ADAM12 was immunoprecipitated from 0.5 mg A431 (human epidermoid carcinoma cell line) whole cell lysate using 0.5 μL ab276047 and 15 μl of 50% Protein G agarose.

Primary antibody : ab276047, at 1/500 dilution
Secondary antibody : Dylight 800-labeled antibody to rabbit IgG (H+L), at 1/5000 dilution

Performed under reducing conditions.

All lanes:

Immunoprecipitation - Anti-ADAM12 antibody [737] - BSA and Azide free (ab276047)

Predicted band size: 100 kDa

false

Western blot - Anti-ADAM12 antibody [737] - BSA and Azide free (AB276047)
  • WB

Unknown

Western blot - Anti-ADAM12 antibody [737] - BSA and Azide free (AB276047)

Performed under reducing conditions.

All lanes:

Western blot - Anti-ADAM12 antibody [737] - BSA and Azide free (ab276047) at 1/500 dilution

All lanes:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

Predicted band size: 100 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

737

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

WB, IP

applications

免疫原

Recombinant Fragment Protein within Human ADAM12 aa 1-550. The exact immunogen used to generate this antibody is proprietary information.

O43184

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>0.2-1 μL/mg of lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ADAM12 also known as meltrin-alpha is a member of the ADAM (a disintegrin and metalloproteinase) family and has a mass of approximately 110 kDa. It functions primarily in the modulation of cell-cell and cell-matrix interactions due to its metalloprotease and disintegrin domains. ADAM12 protein is expressed in various tissues including muscle and placenta. Although found in many cell types it is particularly abundant during embryogenesis and development reflecting its role in these processes.
Biological function summary

The ADAM12 protein holds a role in cell signaling migration and proliferation. It is often part of complexes that interact with other extracellular matrix components influencing cell adhesion and motility. Through its protease activity ADAM12 facilitates growth factor signaling by cleaving membrane-bound precursor proteins to release active growth factors. This activity plays an important role in tissue remodeling and repair.

Pathways

The ADAM12 protein is key in the EGFR signaling pathway where it modulates the availability of epidermal growth factor (EGF) by shedding EGF-like ligands from the cell surface. This action connects ADAM12 with proteins such as ERBB receptors influencing cell division and growth. Another significant pathway is the TGF-beta signaling pathway where ADAM12's interaction with latent TGF-beta complexes can impact the bioavailability of this cytokine therefore relating to proliferation and differentiation of cells.

ADAM12 is linked to cancer progression and metastasis as its activity can positively influence tumor cell invasiveness and growth. Elevated levels of ADAM12 are observed in certain cancer types including breast and prostate cancer. Additionally it associates with metabolic disorders such as obesity where ADAM12 impacts adipogenesis and insulin sensitivity. In cancer ADAM12 interacts with proteins like matrix metalloproteinases (MMPs) to remodel the tumor microenvironment promoting cancer cell invasion.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors (By similarity).
See full target information ADAM12
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2402299 PubMed39316370

2024

Embryo-Derived Cathepsin B Promotes Implantation and Decidualization by Activating Pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Yuan Li,Ying Wu,Hao-Lan Tang,Ying Wang,Bo Li,Yu-Ying He,Gui-Jun Yan,Zeng-Ming Yang
View all publications
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組合せ製品

Assay Kits

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Primary Antibodies

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Secondary Antibodies

AB150077

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Assay Kits

AB171346

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  • 0
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Primary Antibodies

AB8227

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Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
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