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AB18104

Anti-Ada protein 抗体 [ADA-1]

Anti-Ada protein antibody [ADA-1]

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(3 Publications)

Mouse Monoclonal ADA antibody. Suitable for IP, WB, RIA and reacts with Escherichia coli samples. Cited in 3 publications.

別名を表示する

b2213, JW2201, ada, Bifunctional transcriptional activator/DNA repair enzyme Ada, Regulatory protein of adaptive response

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

ADA-1

アイソタイプ

Unknown

キャリアフリー

No

交差種

Escherichia coli

アプリケーション

WB, RIA, IP

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "RIA" : {"fullname" : "Radioimmunoassay", "shortname":"RIA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "RIA-species-checked": "guaranteed", "RIA-species-dilution-info": "", "RIA-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A/G
バッファー組成
Preservative: 0.02% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The Ada protein also known as Ada1 plays a significant role in the bacterial adaptive response to alkylation damage. It acts as a DNA methyltransferase specifically responsible for repairing methylated bases in DNA. Ada protein has an approximate mass of 39 kilodaltons. It is expressed within many bacterial species with E. coli being a common example where its function is well-studied. Ada modulates its activity through a two-step process involving the transfer of methyl groups from damaged DNA to its own cysteine and lysine residues.
Biological function summary

Ada protein serves to directly repair methylated DNA lesions which prevents mutation and maintains genetic stability. It often functions as part of a regulatory complex that initiates the expression of other DNA repair enzymes. The Ada protein acts as both a sensor and a regulator by increasing the transcription of genes involved in the adaptive response making it a central player in the cellular defense against alkylating agents.

Pathways

Ada protein's function links closely with the DNA damage repair pathways. It primarily associates with the base excision repair (BER) pathway to correct lesions caused by endogenous and exogenous agents. Its interaction with other components such as the AlkB protein highlights its role in an interconnected network aiming to maintain the integrity of the genetic material.

Ada protein's impairment correlates with increased mutation rates and potential onset of diseases like cancer due to inadequate DNA repair mechanisms. Although primarily studied in bacterial contexts its analogy in human systems particularly related proteins such as MGMT (O6-methylguanine-DNA methyltransferase) highlights its importance in understanding tumor resistance to alkylating chemotherapy agents. Understanding Ada and similar proteins provide insights into mechanisms that safeguard cellular genomes against mutagenic threats.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Involved in the adaptive response to alkylation damage in DNA caused by alkylating agents. Repairs O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme (Cys-321). Also specifically repairs the Sp diastereomer of DNA methylphosphotriester lesions by the same mechanism, although the methyl transfer occurs onto a different cysteine residue (Cys-38). Cannot demethylate the other diastereomer, Rp-methylphosphotriester. This is a suicide reaction : the enzyme is irreversibly inactivated.. The methylation of Ada by methylphosphotriesters in DNA leads to its activation as a transcriptional regulator that activates the transcription of its own gene, ada, and other alkylation resistance genes, alkA, alkB and aidB.
See full target information ada
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 6:101997 PubMed40054457

2025

siRNA-AGO2 complex inhibits bacterial gene translation: A promising therapeutic strategy for superbug infection.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Wang,Wangjian Sheng,Yu Zhou,Xudong Hang,Jiayi Zhao,Yuanyuan Gu,Xiangfeng Meng,Yuefan Bai,Weili Li,Yujing Zhang,Linlin Zhang,Jing Yu,Zhen Zhou,Xiaona Li,Haorui Sun,Yanhong Xue,Tao Xu,Ke Zen,Hong Ling,Chen-Yu Zhang,Hongkai Bi,Huan Wang

eLife 4: PubMed26402460

2015

Perforin-2 is essential for intracellular defense of parenchymal cells and phagocytes against pathogenic bacteria.

Applications

WB

Species

Unspecified reactive species

Ryan M McCormack,Lesley R de Armas,Motoaki Shiratsuchi,Desiree G Fiorentino,Melissa L Olsson,Mathias G Lichtenheld,Alejo Morales,Kirill Lyapichev,Louis E Gonzalez,Natasa Strbo,Neelima Sukumar,Olivera Stojadinovic,Gregory V Plano,George P Munson,Marjana Tomic-Canic,Robert S Kirsner,David G Russell,Eckhard R Podack

Carcinogenesis 12:263-8 PubMed1995192

1991

Environmental mutagens that induce the adaptive response to alkylating agents in Escherichia coli.

Applications

Unspecified application

Species

Unspecified reactive species

P Vaughan,B Sedgwick,J Hall,J Gannon,T Lindahl
View all publications
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組合せ製品

Assay Kits

AB211093

Adenosine Deaminase (ADA) Activity Assay Kit (Colorimetric)

Functional Studies - Adenosine Deaminase (ADA) Activity Assay Kit (Colorimetric) (AB211093)

  • 5
  • 1
Primary Antibodies

AB79714

Anti-Adenosine Receptor A2a antibody [7F6-G5-A2]

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (AB79714)

  • 4
  • 3
Assay Kits

AB204695

Adenosine Deaminase (ADA) Activity Assay Kit (Fluorometric)

Functional Studies - Adenosine Deaminase (ADA) Activity Assay Kit (Fluorometric) (AB204695)

  • 4
  • 1
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Biochemicals

AB141264

Zebularine, DNA methyltransferase (DNMT) inhibitor

Chemical Structure - Zebularine, DNA methyltransferase (DNMT) inhibitor (AB141264)

  • 0
  • 0

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