Anti-AcV5 tag 抗体 [AcV5] (ab49581)
Key features and details
- Mouse monoclonal [AcV5] to AcV5 tag
- Suitable for: IP, ICC, Dot blot
- Reacts with: Species independent
- Isotype: IgG2b
製品の概要
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製品名
Anti-AcV5 tag antibody [AcV5] -
製品の詳細
Mouse monoclonal [AcV5] to AcV5 tag -
由来種
Mouse -
アプリケーション
適用あり: IP, ICC, Dot blotmore details -
種交差性
交差種: Species independent -
免疫原
Tissue, cells or virus corresponding to AcV5 tag. AcNPV extracellular non occluded virus (NOV)
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ポジティブ･コントロール
- Extracts of Sf9 cells infected with baculovirus.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
ポリ/モノ
モノクローナル -
クローン名
AcV5 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab49581の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at an assay dependent dilution.
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| ICC |
Use at an assay dependent dilution.
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| Dot blot |
Use a concentration of 0.5 - 1 µg/ml.
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| 特記事項 |
|---|
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IP
Use at an assay dependent dilution. |
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ICC
Use at an assay dependent dilution. |
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Dot blot
Use a concentration of 0.5 - 1 µg/ml. |
ターゲット情報
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関連性
Epitope tagged fusion proteins such as AcV5 tag are widely used in protein analysis of biological samples (e.g. plant cell extracts). Antibodies specific for the tag are a valuable means for the detection of the protein. AcV5 recognizes a nine amino acid residue tag of baculovirus Autographa californica GP64 envelope fusion protein (efp). GP64 gene open reading frame encodes a protein of 509 amino acids with a molecular weight of 58 kDa. The protein contains N-linked glycosylation sites and hydrophobic N- and C-termini, characteristic of signal and membrane anchor motifs found in envelope glycoproteins. The GP64 protein is present early (6 hr) post infection and accumulates in the infected cell moving to the periphery later.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab49581 は 3 報の論文で使用されています。
- Ermakova M et al. Installation of C4 photosynthetic pathway enzymes in rice using a single construct. Plant Biotechnol J 19:575-588 (2021). PubMed: 33016576
- Tse DH et al. Designed architectural proteins that tune DNA looping in bacteria. Nucleic Acids Res 49:10382-10396 (2021). PubMed: 34478548
- van Rijn S et al. Multiplex functional bioluminescent reporters using Gaussia luciferase fused to epitope tags in an immunobinding assay. Methods Mol Biol 1098:231-47 (2014). PubMed: 24166381