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リコンビナントRabMAb

Anti-ACTN3 抗体 [EP2531Y] (ab68204)

Reviews (4)Q&A (1)References (5)
Western blot - Anti-ACTN3 antibody [EP2531Y] (ab68204)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody [EP2531Y] (ab68204)
  • Flow Cytometry (Intracellular) - Anti-ACTN3 antibody [EP2531Y] (ab68204)
  • Western blot - Anti-ACTN3 antibody [EP2531Y] (ab68204)
  • Western blot - Anti-ACTN3 antibody [EP2531Y] (ab68204)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody [EP2531Y] (ab68204)
  • Anti-ACTN3 antibody [EP2531Y] (ab68204)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2531Y] to ACTN3
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free

製品の概要

  • 製品名

    Anti-ACTN3 antibody [EP2531Y]
    ACTN3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP2531Y] to ACTN3

  • 由来種

    Rabbit

  • 特異性

    The mouse and  rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-Pmore details
    適用なし: IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab127135)

  • ポジティブ･コントロール

    • WB: Human skeletal muscle, Mouse skeletal muscle, and Rat skeletal muscle lysates. IHC-P: human skeletal muscle tissue. Flow Cyt (intra): L6 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab68204の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (1)
1/5000 - 1/10000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).Can be blocked with Recombinant Human ACTN3 protein (ab127135).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

The mouse and  rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

See IHC antigen retrieval protocols.
特記事項
Flow Cyt (Intra)
1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/5000 - 1/10000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).Can be blocked with Recombinant Human ACTN3 protein (ab127135).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

The mouse and  rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

See IHC antigen retrieval protocols.
追加情報
Is unsuitable for IP.

ターゲット情報

  • 機能

    F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

  • 組織特異性

    Expressed only in a subset of type 2 skeletal muscle fibers.

  • 配列類似性

    Belongs to the alpha-actinin family.
    Contains 1 actin-binding domain.
    Contains 2 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
    Contains 4 spectrin repeats.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Actinin alpha 3 antibody
    • actinin, alpha antibody
    • ACTN 3 antibody
    • ACTN3 antibody
    • ACTN3_HUMAN antibody
    • Alpha actinin skeletal muscle antibody
    • Alpha actinin skeletal muscle isoform 3 antibody
    • Alpha-Actinin 3 antibody
    • Alpha-actinin skeletal muscle isoform 3 antibody
    • Alpha-actinin-3 antibody
    • F actin cross linking protein antibody
    • F-actin cross-linking protein antibody
    • MGC117002 antibody
    • MGC117005 antibody
    • OTTHUMP00000219049 antibody
    • OTTHUMP00000219054 antibody
    see all

画像

  • Western blot - Anti-ACTN3 antibody [EP2531Y] (ab68204)
    Western blot - Anti-ACTN3 antibody [EP2531Y] (ab68204)
    All lanes : Anti-ACTN3 antibody [EP2531Y] (ab68204) at 1/20000 dilution (Purified)

    Lane 1 : Mouse skeletal muscle lysates
    Lane 2 : Rat skeletal muscle lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 103 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody [EP2531Y] (ab68204)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody [EP2531Y] (ab68204)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue sections labeling ACTN3 with purified ab68204 at 1/250 dilution (0.97 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Flow Cytometry (Intracellular) - Anti-ACTN3 antibody [EP2531Y] (ab68204)
    Flow Cytometry (Intracellular) - Anti-ACTN3 antibody [EP2531Y] (ab68204)

    Intracellular Flow Cytometry analysis of L6 (Rat skeletal muscle myoblast) cells labeling ACTN3 with purified ab68204 at 1/30 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

     

  • Western blot - Anti-ACTN3 antibody [EP2531Y] (ab68204)
    Western blot - Anti-ACTN3 antibody [EP2531Y] (ab68204)
    All lanes : Anti-ACTN3 antibody [EP2531Y] (ab68204) at 1/5000 dilution (Purified)

    Lane 1 : Human skeletal muscle lysates at 15 µg


    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 103 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-ACTN3 antibody [EP2531Y] (ab68204)
    Western blot - Anti-ACTN3 antibody [EP2531Y] (ab68204)
    Anti-ACTN3 antibody [EP2531Y] (ab68204) at 1/10000 dilution ((unpurified)) + human skeletal muscle lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 103 kDa
    Observed band size: 103 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody [EP2531Y] (ab68204)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTN3 antibody [EP2531Y] (ab68204)

    Immunohistochemical analysis of paraffin-embedded human muscle tissue using unpurified ab68204 at 1/100-1/250 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-ACTN3 antibody [EP2531Y] (ab68204)
    Anti-ACTN3 antibody [EP2531Y] (ab68204)

参考文献 (5)

ab68204 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab68204 は 5 報の論文で使用されています。

  • Nieves-Rodriguez S  et al. Transcriptomic analysis of paired healthy human skeletal muscles to identify modulators of disease severity in DMD. Front Genet 14:1216066 (2023). PubMed: 37576554
  • Wyckelsma VL  et al. Loss of a-actinin-3 during human evolution provides superior cold resilience and muscle heat generation. Am J Hum Genet 108:446-457 (2021). PubMed: 33600773
  • Seo JY  et al. Maintenance of type 2 glycolytic myofibers with age by Mib1-Actn3 axis. Nat Commun 12:1294 (2021). PubMed: 33637766
  • Lornage X  et al. ACTN2 mutations cause "Multiple structured Core Disease" (MsCD). Acta Neuropathol 137:501-519 (2019). PubMed: 30701273
  • Koskinen SOA  et al. Human skeletal muscle type 1 fibre distribution and response of stress-sensing proteins along the titin molecule after submaximal exhaustive exercise. Histochem Cell Biol 148:545-555 (2017). PubMed: 28712031

レビューと Q&A

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1-5 of 5 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-ACTN3 antibody [EP2531Y]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Permeabilization
Yes - Tween-20
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Aug 22 2016

Immunohistochemistry (Frozen sections) abreview for Anti-ACTN3 antibody [EP2531Y]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Cell (skeletal muscle/frozen sections, human, rat and mo)
Permeabilization
Yes - 0,1 % Triton X-100 in PBS
Specification
skeletal muscle/frozen sections, human, rat and mo
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Histofix/Histolab/Sweden
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Satu Koskinen

Verified customer

投稿 Aug 01 2016

Western blot abreview for Anti-ACTN3 antibody [EP2531Y]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal Muscle)
Gel Running Conditions
Reduced Denaturing (4%-12%)
Loading amount
10 µg
Specification
Skeletal Muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 25 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-ACTN3 antibody [EP2531Y]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Permeabilization
Yes - saponin
Specification
Human pluripotent stem cell derived cardiomyocyte
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 21 2015

Question

Hello,
Do you know if the Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330) can be used a reliable positive control for the Anti-ACTN3 [EP2531Y] (ab68204) antibody in western blotting?
Thank you

Read More
Answer

Thank you for contacting us.
Skeletal Muscle (Human) Tissue Lysate is recommended as a positive control for ab68204. Both products are recommended for Western Blotting, and are guaranteed to work in this application.
If you had any problems using these products in the applications and species stated on our datasheet, please do not hesitate to contact us. Our Abpromise guarantees they should work in these conditions, and if they didn’t, we would replace or refund them, if reported within 6 months after purchasing.
I hope this helps. Please do not hesitate to contact us for further assistance.

Read More

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