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    products/primary-antibodies/acth-antibody-ab74976.pdf

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Anti-ACTH 抗体 (ab74976)

  • Datasheet
  • SDS
Reviews (4)Q&A (2)References (25)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody (ab74976)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody (ab74976)

Key features and details

  • Rabbit polyclonal to ACTH
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-POMC antibody [EPR22534-165] (ab254257)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-ACTH antibody
    ACTH 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to ACTH
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: IHC-Pmore details
  • 種交差性

    交差種: Mouse, Rat, Human
    交差が予測される動物種: Sheep, Goat, Chicken, Guinea pig, Cow, Dog, Chimpanzee, Macaque monkey, Orangutan
  • 免疫原

    Synthetic peptide corresponding to ACTH aa 1-39. This corresponds to amino acids 138-176 of the precursor POMC.
    Sequence:

    SYSMEHFRWGKPVGKKRRPVKVYPNGAEDESAEAFPLEF


    Database link: P01189
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Neurotransmitter
    • Neuropeptides
    • Hormones
    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Neuroscience
    • Endocrine system
    • Hypothalamic pituitary adrenal axis
    • Cancer
    • Tumor biomarkers
    • Hormones
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab74976の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (3)
Use at an assay dependent concentration.
特記事項
IHC-P
Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    ACTH occurs in cells of the anterior pituitary and in neurons in brain. It regulates the corticosteroid production in the adrenal cortex. Beta endorphin and Met enkephalin are endogenous opiates. MSH (melanocyte stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes.
  • 細胞内局在

    Secreted
  • 参照データベース

    • Entrez Gene: 5443 Human
    • Entrez Gene: 694430 Macaque monkey
    • Entrez Gene: 18976 Mouse
    • Entrez Gene: 24664 Rat
    • Entrez Gene: 443212 Sheep
    • Omim: 176830 Human
    • SwissProt: P01190 Cow
    • SwissProt: P19402 Guinea pig
    • SwissProt: P01189 Human
    • SwissProt: P01201 Macaque monkey
    • SwissProt: P01193 Mouse
    • SwissProt: P01194 Rat
    • SwissProt: P01191 Sheep
    see all
  • 別名

    • ACTH antibody
    • Adrenocorticotropic hormone antibody
    • Adrenocorticotropin antibody
    • Alpha melanocyte stimulating hormone antibody
    • alpha-MSH antibody
    • alphaMSH antibody
    • Beta LPH antibody
    • Beta melanocyte stimulating hormone antibody
    • Beta-endorphin antibody
    • beta-MSH antibody
    • CLIP antibody
    • Corticotropin antibody
    • Corticotropin lipotropin antibody
    • Corticotropin-like intermediary peptide antibody
    • Gamma LPH antibody
    • gamma-MSH antibody
    • Lipotropin beta antibody
    • Lipotropin gamma antibody
    • Lipotropin, included antibody
    • LPH antibody
    • Melanocyte-stimulating hormone, included antibody
    • Melanotropin alpha antibody
    • Melanotropin beta antibody
    • Melanotropin gamma antibody
    • Melanotropin, included antibody
    • Met-enkephalin antibody
    • MSH antibody
    • NPP antibody
    • Opiomelanocortin prepropeptide antibody
    • POC antibody
    • POMC antibody
    • Pomc-1 antibody
    • Pomc1 antibody
    • Pomc2 antibody
    • Pro ACTH endorphin antibody
    • Pro opiomelanocortin antibody
    • Pro-opiomelanocortin-alpha antibody
    • Proopiomelanocortin antibody
    • Proopiomelanocortin preproprotein antibody
    • Tetracosactide antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody (ab74976)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody (ab74976)

    Formalin-fixed, paraffin-embedded mouse brain tissue stained for ACTH with ab74976 (1/100 dilution) in immunohistochemical analysis. DAB (brown) and Hematoxylin (blue)staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody (ab74976)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTH antibody (ab74976)Image from Zhang Z et al, J Biol Chem. 2010 Nov 5;285(45):34718-28. Epub 2010 Aug 31, Fig 4. DOI 10.1074/jbc.M110.126441

    ab74976 staining ACTH in P0 Pitx2-Cre/Dicer1 mutant mice pituitary tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Samples were fixed in 4% paraformaldehyde, dehydrated, and embedded in paraffin wax. Sections were cut (7 µm) and stained with H&E. Immunohistochemistry was performed on 7-µm paraffin sections stained by an indirect immunoperoxidase method. Peroxidase activity was visualized with AEC stain kit. ab74976 diluted 1/200. Secondary antibodies conjugated with biotin were used at 1/150 dilution.

プロトコール

  • Immunohistochemistry protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (25)

ab74976 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab74976 は 25 報の論文で使用されています。

  • Urai S  et al. Paraneoplastic isolated adrenocorticotropic hormone deficiency revealed after immune checkpoint inhibitors therapy: new insights into anti-corticotroph antibody. Front Immunol 14:1284301 (2023). PubMed: 38035072
  • Liu M  et al. Evidence for Neuropeptide W Acting as a Physiological Corticotropin-releasing Inhibitory Factor in Male Chickens. Endocrinology 163:N/A (2022). PubMed: 35583189
  • Wan Y  et al. Characterization of CRH-Binding Protein (CRHBP) in Chickens: Molecular Cloning, Tissue Distribution and Investigation of Its Role as a Negative Feedback Regulator within the Hypothalamus-Pituitary-Adrenal Axis. Genes (Basel) 13:N/A (2022). PubMed: 36292565
  • Royan MR  et al. 3D Atlas of the Pituitary Gland of the Model Fish Medaka (Oryzias latipes). Front Endocrinol (Lausanne) 12:719843 (2021). PubMed: 34497587
  • LaPierre MP  et al. MicroRNA-7a2 Regulates Prolactin in Developing Lactotrophs and Prolactinoma Cells. Endocrinology 162:N/A (2021). PubMed: 33248443
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-6 of 6 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ACTH antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (pituitary)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
Yes - methanol
Specification
pituitary
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

祥代 田中

Verified customer

投稿 Jun 13 2025

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ACTH antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (pituitary)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid buffer
Permeabilization
No
Specification
pituitary
Blocking step
Milk as blocking agent for 40 minute(s) · Concentration: 8% · Temperature: 37°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 05 2022

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ACTH antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Medaka fish Tissue sections (Pituitary)
Antigen retrieval step
None
Permeabilization
Yes - 10 minutes in 0.3% Triton (diluted in 1X PBST))
Specification
Pituitary
Blocking step
Normal Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Muhammad Rahmad Royan

Verified customer

投稿 Apr 02 2020

Western blot abreview for Anti-ACTH antibody

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Pig Purified protein (brain)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
10 µg
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 27 2015

Question

Dear All,
do you have any WB/IP images of your antibody AB74976?
If you do, can you please share with us these images?

Thanks in advance
kind regards

Read More

Abcam community

Verified customer

Asked on Feb 18 2013

Answer

Thank you for contacting us.

Unfortunately we do not have any images for sharing we however can provide 100% image discount, if customer tests the antibody and submit an image in the form of Abreview. Please let me know if you want an Abtrial code.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Feb 18 2013

Question

Can you please help with the following enquiry?
I am wanting to purchase polyclonal antibodies to the synthetic ACTH (1-24):
ab74976 (antigen 1-39)
ab8906 (antigen 1-39 IgG)
ab2407 (antigen 1-24, whole serum)
ab75683 (antigen 1-39, IgG)
Can you offer an suggestions as to which one would be most appropriate for my application - I am wanting to bind the antibody to magnetic beads that have been coated with a second antibody which will work with a primary antibody of rabbit origin. I then want to be able to separate the isolated ACTH (1-39) and ACTH (1-24) and analyse by LCMS.
Your advice is much appreciated J

Kind regards,

Read More

Abcam community

Verified customer

Asked on Feb 04 2013

Answer

Thank you for contacting us.

You can use any antibody however we will not be able to provide details about the purity of isolated protein.

The antibody ab2407 is in whole antiserum which means the concentration is not available so the optimization of the IP dilution would be necessary for better results.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Feb 04 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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