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リコンビナントRabMAb

Anti-ACTC1 抗体 [EP184E] (ab46805)

Submit a review Submit a question References (23)
Western blot - Anti-ACTC1 antibody [EP184E] (ab46805)
  • Immunocytochemistry/ Immunofluorescence - Anti-ACTC1 antibody [EP184E] (ab46805)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTC1 antibody [EP184E] (ab46805)
  • Flow Cytometry (Intracellular) - Anti-ACTC1 antibody [EP184E] (ab46805)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTC1 antibody [EP184E] (ab46805)
  • Anti-ACTC1 antibody [EP184E] (ab46805)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP184E] to ACTC1
  • Suitable for: Flow Cyt (Intra), IHC-FoFr, IHC-P, ICC/IF, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free HRP

製品の概要

  • 製品名

    Anti-ACTC1 antibody [EP184E]
    ACTC1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP184E] to ACTC1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), IHC-FoFr, IHC-P, ICC/IF, WBmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • A431 cell lysate; HeLa cells; human cardiac muscle

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab46805の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-FoFr
Use at an assay dependent concentration.
IHC-P
1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
WB
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
特記事項
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-FoFr
Use at an assay dependent concentration.
IHC-P
1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
WB
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

ターゲット情報

  • 機能

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

  • 関連疾患

    Defects in ACTC1 are the cause of cardiomyopathy dilated type 1R (CMD1R) [MIM:613424]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in ACTC1 are the cause of cardiomyopathy familial hypertrophic type 11 (CMH11) [MIM:612098]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in ACTC1 are the cause of atrial septal defect type 5 (ASD5) [MIM:612794]. A congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria.

  • 配列類似性

    Belongs to the actin family.

  • 細胞内局在

    Cytoplasm > cytoskeleton.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • ACTC antibody
    • ACTC_HUMAN antibody
    • ACTC1 antibody
    • Actin alpha cardiac muscle 1 antibody
    • Actin antibody
    • alpha cardiac muscle 1 antibody
    • Alpha-cardiac actin antibody
    • ASD5 antibody
    • CMD1R antibody
    • CMH11 antibody
    • LVNC4 antibody
    see all

画像

  • Western blot - Anti-ACTC1 antibody [EP184E] (ab46805)
    Western blot - Anti-ACTC1 antibody [EP184E] (ab46805)
    Anti-ACTC1 antibody [EP184E] (ab46805) at 1/1000 dilution + A431 cell lysate at 10ug/lane

    Secondary
    Goat anti-rabbit, HRP labelled. at 1/2000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ACTC1 antibody [EP184E] (ab46805)
    Immunocytochemistry/ Immunofluorescence - Anti-ACTC1 antibody [EP184E] (ab46805)
    Immunofluorescent staining of HeLa cells using ab46805 at a 1/250 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTC1 antibody [EP184E] (ab46805)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTC1 antibody [EP184E] (ab46805)

    Immunohistochemical staining of paraffin-embedded human cardiac muscle using ab46805 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-ACTC1 antibody [EP184E] (ab46805)
    Flow Cytometry (Intracellular) - Anti-ACTC1 antibody [EP184E] (ab46805)

    Overlay histogram showing HeLa cells stained with ab46805 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab46805, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTC1 antibody [EP184E] (ab46805)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTC1 antibody [EP184E] (ab46805)

    Fluorescent immunohistochemical analysis of paraffin-embedded human smooth muscle tissue using ab46805. Green-specific fluorescent staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-ACTC1 antibody [EP184E] (ab46805)
    Anti-ACTC1 antibody [EP184E] (ab46805)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (23)

ab46805 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab46805 は 23 報の論文で使用されています。

  • Kra G  et al. Alpha-linolenic acid modulates systemic and adipose tissue-specific insulin sensitivity, inflammation, and the endocannabinoid system in dairy cows. Sci Rep 13:5280 (2023). PubMed: 37002295
  • Daddam JR  et al. Plant polyphenol extract supplementation affects performance, welfare, and the Nrf2-oxidative stress response in adipose tissue of heat-stressed dairy cows. J Dairy Sci 106:9807-9821 (2023). PubMed: 37641328
  • Kra G  et al. Effects of Environmental Heat Load on Endocannabinoid System Components in Adipose Tissue of High Yielding Dairy Cows. Animals (Basel) 12:N/A (2022). PubMed: 35327191
  • Daddam JR  et al. Phosphoproteomic Analysis of Subcutaneous and Omental Adipose Tissue Reveals Increased Lipid Turnover in Dairy Cows Supplemented with Conjugated Linoleic Acid. Int J Mol Sci 22:N/A (2021). PubMed: 33810070
  • Das S  et al. Pre-innervated tissue-engineered muscle promotes a pro-regenerative microenvironment following volumetric muscle loss. Commun Biol 3:330 (2020). PubMed: 32587337
View all Publications for this product

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