Anti-ACSL3 抗体 (ab151959)
Key features and details
- Rabbit polyclonal to ACSL3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ACSL3 antibody
ACSL3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ACSL3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Pig
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免疫原
Recombinant fragment, corresponding to a region within amino acids 416-637 of Human ACSL3 (O95573).
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ポジティブ･コントロール
- 293T, A431, Jurkat and Raji whole cell lysates, Human Breast carcinoma tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab151959の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 80 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 80 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis (By similarity). Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates (By similarity). Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins). -
配列類似性
Belongs to the ATP-dependent AMP-binding enzyme family. -
細胞内局在
Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2181 Human
- Entrez Gene: 74205 Mouse
- Entrez Gene: 100233169 Pig
- Entrez Gene: 114024 Rat
- Omim: 602371 Human
- SwissProt: O95573 Human
- SwissProt: Q9CZW4 Mouse
- SwissProt: Q63151 Rat
see all -
別名
- ACS3 antibody
- Acsl3 antibody
- ACSL3_HUMAN antibody
see all
画像
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Western blot - Anti-ACSL3 antibody (ab151959)All lanes : Anti-ACSL3 antibody (ab151959) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 80 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL3 antibody (ab151959)Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labeling ACSL3 with ab151959 antibody at 1/500.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab151959 は 6 報の論文で使用されています。
- Wang N et al. USP7- and PRMT5-dependent G3BP2 stabilization drives de novo lipogenesis and tumorigenesis of HNSC. Cell Death Dis 14:182 (2023). PubMed: 36878903
- Sakamoto T et al. The nuclear receptor ERR cooperates with the cardiogenic factor GATA4 to orchestrate cardiomyocyte maturation. Nat Commun 13:1991 (2022). PubMed: 35418170
- Ma Y et al. Long-Chain Acyl-CoA Synthetase 4-Mediated Fatty Acid Metabolism Sustains Androgen Receptor Pathway-Independent Prostate Cancer. Mol Cancer Res 19:124-135 (2021). PubMed: 33077484
- Sun X et al. The modification of ferroptosis and abnormal lipometabolism through overexpression and knockdown of potential prognostic biomarker perilipin2 in gastric carcinoma. Gastric Cancer 23:241-259 (2020). PubMed: 31520166
- Kankeu C et al. Quantitative proteomics and systems analysis of cultured H9C2 cardiomyoblasts during differentiation over time supports a 'function follows form' model of differentiation. Mol Omics 14:181-196 (2018). PubMed: 29770421
- Belkaid A et al. 17ß-estradiol-induced ACSL4 protein expression promotes an invasive phenotype in estrogen receptor positive mammary carcinoma cells. Carcinogenesis 38:402-410 (2017). PubMed: 28334272