Anti-ACRBP 抗体 (ab64809)
Key features and details
- Rabbit polyclonal to ACRBP
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ACRBP antibody -
製品の詳細
Rabbit polyclonal to ACRBP -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
A synthetic peptide derived from an internal region of human ACRBP.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64809の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
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| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
May be involved in packaging and condensation of the acrosin zymogen in the acrosomal matrix via its association with proacrosin. -
組織特異性
Expression restricted to testis in normal tissue. Expressed in a wide spectrum of cancers, including bladder, breast, liver, lung and colon cancers. -
翻訳後修飾
The N-terminus is blocked.
Phosphorylated on Tyr residues in capacitated sperm. -
細胞内局在
Secreted. Cytoplasmic vesicle, secretory vesicle, acrosome. Colocalizes with proacrosin in the acrosome of sperm. - Information by UniProt
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参照データベース
- Entrez Gene: 84519 Human
- Omim: 608352 Human
- SwissProt: Q8NEB7 Human
- Unigene: 123239 Human
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別名
- ACRBP antibody
- ACRBP_HUMAN antibody
- acrosin binding protein antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACRBP antibody (ab64809)IHC image of ab64809 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64809, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-ACRBP antibody (ab64809)All lanes : Anti-ACRBP antibody (ab64809) at 1/500 dilution
Lane 1 : Extracts of 293 cells
Lane 2 : Extracts of HepG2 cells
Lane 3 : Extracts of Jurkat cells
Lane 4 : Extracts of Jurkat cells with immunizing Peptide
Lysates/proteins at 5 µg per lane.
Predicted band size: 61 kDa
Observed band size: 61 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab64809 は 4 報の論文で使用されています。
- Lin L et al. Cancer-testis antigen ACRBP expression and serum immunoreactivity in ovarian cancer: Its association with prognosis. Immun Inflamm Dis 9:1759-1770 (2021). PubMed: 34528758
- Liu X et al. iTRAQ-based analysis of sperm proteome from normozoospermic men achieving the rescue-ICSI pregnancy after the IVF failure. Clin Proteomics 15:27 (2018). PubMed: 30166971
- Li X et al. Serum immunoreactivity of cancer/testis antigen OY-TES-1 and its tissues expression in glioma. Oncol Lett 13:3080-3086 (2017). PubMed: 28529561
- Cen YH et al. Knockdown of OY-TES-1 by RNAi causes cell cycle arrest and migration decrease in bone marrow-derived mesenchymal stem cells. Cell Biol Int 36:917-22 (2012). PubMed: 22651134