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リコンビナントRabMAb

Anti-ACOX1/AOX 抗体 [EPR19038] (ab184032)

Submit a review Submit a question References (34)
Western blot - Anti-ACOX1/AOX antibody [EPR19038] (ab184032)
  • Western blot - Anti-ACOX1/AOX antibody [EPR19038] (ab184032)
  • Western blot - Anti-ACOX1/AOX antibody [EPR19038] (ab184032)
  • Immunocytochemistry/ Immunofluorescence - Anti-ACOX1/AOX antibody [EPR19038] (ab184032)
  • Immunocytochemistry/ Immunofluorescence - Anti-ACOX1/AOX antibody [EPR19038] (ab184032)
  • Flow Cytometry (Intracellular) - Anti-ACOX1/AOX antibody [EPR19038] (ab184032)
  • Immunoprecipitation - Anti-ACOX1/AOX antibody [EPR19038] (ab184032)
  • Anti-ACOX1/AOX antibody [EPR19038] (ab184032)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19038] to ACOX1/AOX
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-ACOX1/AOX antibody [EPR19038]
    ACOX1/AOX 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR19038] to ACOX1/AOX

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, ICC/IF, IPmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse liver, heart and kidney lysates; C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Rat brain, heart and liver lysates; Human fetal liver and fetal kidney lysates. ICC/IF: C6 and NIH/3T3 cells. Flow Cyt (intra): NIH/3T3 cells. IP: NIH/3T3 whole cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184032の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/100.
WB
1/1000. Detects a band of approximately 72, 50 kDa (predicted molecular weight: 75 kDa).
ICC/IF
1/250.
IP
1/30.
特記事項
Flow Cyt (Intra)
1/100.
WB
1/1000. Detects a band of approximately 72, 50 kDa (predicted molecular weight: 75 kDa).
ICC/IF
1/250.
IP
1/30.

ターゲット情報

  • 機能

    Catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. Isoform 1 shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length. Isoform 2 is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs. Isoform 2 is twice as active as isoform 1 against 16-hydroxy-palmitoyl-CoA and is 25% more active against 1,16-hexadecanodioyl-CoA.

  • 組織特異性

    Widely expressed with highest levels of isoform 1 and isoform 2 detected in testis. Isoform 1 is expressed at higher levels than isoform 2 in liver and kidney while isoform 2 levels are higher in brain, lung, muscle, white adipose tissue and testis. Levels are almost equal in heart.

  • パスウェイ

    Lipid metabolism; peroxisomal fatty acid beta-oxidation.

  • 関連疾患

    Adrenoleukodystrophy, pseudoneonatal

  • 配列類似性

    Belongs to the acyl-CoA oxidase family.

  • 細胞内局在

    Peroxisome.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • ACOX antibody
    • ACOX1 antibody
    • ACOX1_HUMAN antibody
    • Acyl CoA oxidase 1 palmitoyl antibody
    • Acyl CoA oxidase straight chain antibody
    • AOX antibody
    • EC 1.3.3.6 antibody
    • PALMCOX antibody
    • Palmitoyl CoA oxidase antibody
    • Palmitoyl-CoA oxidase antibody
    • Peroxisomal acyl coenzyme A oxidase 1 antibody
    • Peroxisomal acyl-coenzyme A oxidase 1 antibody
    • Peroxisomal fatty acyl CoA oxidase antibody
    • SCOX antibody
    • Straight chain acyl CoA oxidase antibody
    • Straight-chain acyl-CoA oxidase antibody
    see all

画像

  • Western blot - Anti-ACOX1/AOX antibody [EPR19038] (ab184032)
    Western blot - Anti-ACOX1/AOX antibody [EPR19038] (ab184032)
    Lanes 1-5 : Anti-ACOX1/AOX antibody [EPR19038] (ab184032) at 1/1000 dilution
    Lanes 6-7 : Anti-ACOX1/AOX antibody [EPR19038] (ab184032) at 1/5000 dilution

    Lane 1 : Mouse liver lysate
    Lane 2 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 3 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
    Lane 6 : Mouse heart lysate
    Lane 7 : Mouse kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 75 kDa
    Observed band size: 50,72 kDa why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST.

    Exposure time Lane 1-5: 1 second; Lane 6: 30 seconds; Lane 7: 2 seconds.

    The expression profile observed is consistent with what has been described in the literature (PMID:) 8798738 and 17255948.

  • Western blot - Anti-ACOX1/AOX antibody [EPR19038] (ab184032)
    Western blot - Anti-ACOX1/AOX antibody [EPR19038] (ab184032)
    All lanes : Anti-ACOX1/AOX antibody [EPR19038] (ab184032) at 1/1000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat heart lysate
    Lane 3 : Rat liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 75 kDa
    Observed band size: 50,72 kDa why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST.

    Exposure time Lane 1/2: 8 seconds;Lane 3: 1 second.

    The expression profile observed is consistent with what has been described in the literature (PMID:) 8798738 and 17255948.

  • Western blot - Anti-ACOX1/AOX antibody [EPR19038] (ab184032)
    Western blot - Anti-ACOX1/AOX antibody [EPR19038] (ab184032)
    All lanes : Anti-ACOX1/AOX antibody [EPR19038] (ab184032) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 75 kDa
    Observed band size: 50,72 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID:) 8798738 and 17255948.

  • Immunocytochemistry/ Immunofluorescence - Anti-ACOX1/AOX antibody [EPR19038] (ab184032)
    Immunocytochemistry/ Immunofluorescence - Anti-ACOX1/AOX antibody [EPR19038] (ab184032)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 (Rat glial tumor cell line) cells labeling ACOX1/AOX with ab184032 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on C6 cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [EPR19038] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab184032 at 1/250 dilution followed by ab150120 at 1/1000 dilution.
    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-ACOX1/AOX antibody [EPR19038] (ab184032)
    Immunocytochemistry/ Immunofluorescence - Anti-ACOX1/AOX antibody [EPR19038] (ab184032)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling ACOX1/AOX with ab184032 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [EPR19038] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab184032 at 1/250 dilution followed by ab150120 at 1/1000 dilution.
    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077  at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-ACOX1/AOX antibody [EPR19038] (ab184032)
    Flow Cytometry (Intracellular) - Anti-ACOX1/AOX antibody [EPR19038] (ab184032)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast) cell linelabeling ACOX1/AOX with ab184032 at 1/100 dilution (red) compared with a Rabbit IgG, monoclonal [EPR19038] - Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody. 

     

     

  • Immunoprecipitation - Anti-ACOX1/AOX antibody [EPR19038] (ab184032)
    Immunoprecipitation - Anti-ACOX1/AOX antibody [EPR19038] (ab184032)

    ACOX1/AOX was immunoprecipitated from 1mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab184032 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184032 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
    Lane 1: NIH/3T3 whole cell lysate 10µg (Input).
    Lane 2: ab184032 IP in NIH/3T3 whole cell lysate.
    Lane 3: Rabbit IgG, monoclonal [EPR19038] - Isotype Control (ab172730) instead of ab184032 in HeLa whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

  • Anti-ACOX1/AOX antibody [EPR19038] (ab184032)
    Anti-ACOX1/AOX antibody [EPR19038] (ab184032)

参考文献 (34)

ab184032 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab184032 は 34 報の論文で使用されています。

  • Yao Y  et al. P38γ modulates the lipid metabolism in non-alcoholic fatty liver disease by regulating the JAK-STAT signaling pathway. FASEB J 37:e22716 (2023). PubMed: 36527390
  • Zakic T  et al. Redox-metabolic reprogramming of skin in mice lacking functional Nrf2 under basal conditions and cold acclimation. Biofactors 49:600-611 (2023). PubMed: 36585756
  • Yau WW  et al. Nicotinamide riboside rescues dysregulated glycolysis and fatty acid β-oxidation in a human hepatic cell model of citrin deficiency. Hum Mol Genet 32:1922-1931 (2023). PubMed: 36881658
  • Chornyi S  et al. The origin of long-chain fatty acids required for de novo ether lipid/plasmalogen synthesis. J Lipid Res 64:100364 (2023). PubMed: 36990386
  • Pellegrino E  et al. Peroxisomal ROS control cytosolic Mycobacterium tuberculosis replication in human macrophages. J Cell Biol 222:N/A (2023). PubMed: 37737955
View all Publications for this product

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