Anti-Aconitase 2 抗体 (ab99467)
Key features and details
- Goat polyclonal to Aconitase 2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Aconitase 2 antibody
Aconitase 2 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Aconitase 2 -
由来種
Goat -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog, Pig
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免疫原
Synthetic peptide:
C-QHVDVSPTSQRLQ
, corresponding to internal sequence amino acids 554-566 of Human Aconitase 2 (NP_001089.1). -
ポジティブ･コントロール
- Human Heart lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab99467 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99467の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| WB |
Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 85 kDa.
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
IHC-P
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 85 kDa. 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. -
パスウェイ
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2. -
配列類似性
Belongs to the aconitase/IPM isomerase family. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 280976 Cow
- Entrez Gene: 474487 Dog
- Entrez Gene: 50 Human
- Entrez Gene: 11429 Mouse
- Entrez Gene: 396999 Pig
- Entrez Gene: 79250 Rat
- Omim: 100850 Human
- SwissProt: P20004 Cow
see all -
別名
- ACO 2 antibody
- ACO2 antibody
- ACON_HUMAN antibody
see all
画像
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Western blot - Anti-Aconitase 2 antibody (ab99467)Anti-Aconitase 2 antibody (ab99467) at 0.01 µg/ml + Human Heart lysates at 35 µg
Predicted band size: 85 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab99467 は 2 報の論文で使用されています。
- Márquez-Jurado S et al. Mitochondrial levels determine variability in cell death by modulating apoptotic gene expression. Nat Commun 9:389 (2018). PubMed: 29374163
- Morton NM et al. Genetic identification of thiosulfate sulfurtransferase as an adipocyte-expressed antidiabetic target in mice selected for leanness. Nat Med 22:771-9 (2016). PubMed: 27270587