AB183721

Anti-Aconitase 1/ACO1 抗体 [EPR7226(2)]

Anti-Aconitase 1/ACO1 antibody [EPR7226(2)]

RabMAb
Recombinant
KO Validated
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(7 Publications)

Rabbit Recombinant Monoclonal Aconitase 1/ACO1 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications.

別名を表示する

IREB1, ACO1, Cytoplasmic aconitate hydratase, Aconitase, Citrate hydro-lyase, Ferritin repressor protein, Iron regulatory protein 1, Iron-responsive element-binding protein 1, IRP1, IRE-BP 1

3 Images

Supplier Data

Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (AB183721)

All lanes:

Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

T.T cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution

Predicted band size: 98 kDa

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Lab

Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (AB183721)

Lanes 1 - 4 : Merged signal (red and green). Green - ab183721 observed at 98 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab183721 was shown to specifically react with ACO1 in wild-type U20S cells as signal was lost in ACO1 knockout cells. Wild-type and ACO1 knockout samples were subjected to SDS-PAGE. ab183721 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721) at 1 µg/mL

Lane 1:

Wild-type U20S whole cell lysate at 20 µg

Lane 2:

ACO1 knockout U-2 OS whole cell lysate at 20 µg

Lane 2:

Western blot - Human ACO1 knockout U-2 OS cell line (<a href='/products/cell-lines/human-aco1-knockout-u-2-os-cell-line-ab261884'>ab261884</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Predicted band size: 98 kDa

false

Supplier Data

Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (AB183721)

All lanes:

Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (ab183721) at 1/20000 dilution

All lanes:

Human fetal liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 98 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR7226(2)

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Aconitase 1 also known as ACO1 or ACOONe is an enzyme with a vital role in cellular metabolism. It weighs approximately 98 kDa and is expressed in the cytosol of cells. Mechanically ACO1 functions as a bifunctional protein participating in the citric acid cycle. Its main role is the reversible isomerization of citrate to isocitrate mediated by the dehydration and rehydration processes. ACO1 requires a 4Fe-4S cluster to be active in its enzymatic form influencing cellular iron metabolism by regulating the levels of iron-responsive elements in mRNA.

Biological function summary

ACO1 connects energy production and iron regulation. It binds to iron-responsive elements when the iron is scarce and acts in its aconitase form when iron levels are sufficient. ACO1 is not part of a larger protein complex but plays an important regulatory role in balancing cellular energy output and iron homeostasis. It affects both glycolysis and the electron transport chain indirectly by altering the availability of citric acid cycle intermediates which are important for ATP production.

Pathways

ACO1 integrates into both the citric acid cycle and the iron regulatory pathway. The citric acid cycle also called the Krebs cycle is fundamental for ATP production and ACO1 serves as a pivotal point within this cycle. Moreover through its iron-regulatory function it connects to iron metabolism pathways that are vital for various cellular processes. ACO1 activity also influences other proteins like ferritin by regulating iron storage and transport mechanisms through its dual functionality.

ACO1 plays a role in certain neurodegenerative diseases and anemia. Dysfunction in ACO1's activity can lead to imbalances in iron metabolism contributing to disorders like Friedreich's ataxia where iron accumulation causes mitochondrial damage. Anemia can result from inadequate iron regulation because of faulty ACO1 function affecting proteins such as transferrin which is important for iron transport in the blood. Understanding ACO1's dual regulatory functions provides essential insights into these disease processes and potential therapeutic approaches.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Bifunctional iron sensor that switches between 2 activities depending on iron availability (PubMed : 1281544, PubMed : 1946430, PubMed : 8041788). Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization (PubMed : 1281544, PubMed : 1946430, PubMed : 23891004, PubMed : 8041788). Binds to iron-responsive elements (IRES) in the untranslated region of target mRNAs preventing for instance the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA (PubMed : 1281544, PubMed : 1946430, PubMed : 23891004, PubMed : 8041788).. Conversely, when cellular iron levels are high, binds a 4Fe-4S cluster which precludes RNA binding activity and promotes the aconitase activity, the isomerization of citrate to isocitrate via cis-aconitate.

See full target information ACO1

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:10838 PubMed39738060

2024

Mammalian SLC39A13 promotes ER/Golgi iron transport and iron homeostasis in multiple compartments.

Applications

Unspecified application

Species

Unspecified reactive species

Huihui Li,Yanmei Cui,Yule Hu,Mengran Zhao,Kuanyu Li,Xiaoyun Pang,Fei Sun,Bing Zhou

iScience 27:108955 PubMed38322996

2024

Exosomal miR-3174 induced by hypoxia promotes angiogenesis and metastasis of hepatocellular carcinoma by inhibiting HIPK3.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Yang,Mingyu Wu,Xiangxu Kong,Yun Wang,Chunyang Hu,Deming Zhu,Lianbao Kong,Fei Qiu,Wangjie Jiang

Nature communications 14:3716 PubMed37349299

2023

Mitochondrial aconitase suppresses immunity by modulating oxaloacetate and the mitochondrial unfolded protein response.

Applications

Unspecified application

Species

Unspecified reactive species

Eunah Kim,Andrea Annibal,Yujin Lee,Hae-Eun H Park,Seokjin Ham,Dae-Eun Jeong,Younghun Kim,Sangsoon Park,Sujeong Kwon,Yoonji Jung,JiSoo Park,Sieun S Kim,Adam Antebi,Seung-Jae V Lee

Thoracic cancer 14:1477-1491 PubMed37144254

2023

Tumor-derived exosomal miR-148b-3p mediates M2 macrophage polarization via TSC2/mTORC1 to promote breast cancer migration and invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Hao,Zhimei Sheng,Wenhao Wang,Ruijun Feng,Yuanhang Zheng,Qinpei Xiao,Baogang Zhang

Frontiers in pharmacology 14:1127931 PubMed37006994

2023

Kumatakenin inhibited iron-ferroptosis in epithelial cells from colitis mice by regulating the Eno3-IRP1-axis.

Applications

Unspecified application

Species

Unspecified reactive species

Arenbaoligao,Xinrui Guo,Jiahao Xiong,Shuangshuang Zhang,Yuewen Yang,Dapeng Chen,Yu Xie

Scientific reports 12:21483 PubMed36509805

2022

Paraoxonase-2 contributes to promoting lipid metabolism and mitochondrial function via autophagy activation.

Applications

Unspecified application

Species

Unspecified reactive species

Gu-Choul Shin,Hyeong Min Lee,Nayeon Kim,Sang-Ku Yoo,Hyung Soon Park,Leo Sungwong Choi,Kwang Pyo Kim,Ah-Ra Lee,Sang-Uk Seo,Kyun-Hwan Kim

Cell reports 35:108983 PubMed33852870

2021

A naturally occurring mutation in ATP synthase subunit c is associated with increased damage following hypoxia/reoxygenation in STEMI patients.

Applications

Unspecified application

Species

Unspecified reactive species

Giampaolo Morciano,Gaia Pedriali,Massimo Bonora,Rita Pavasini,Elisa Mikus,Simone Calvi,Matteo Bovolenta,Magdalena Lebiedzinska-Arciszewska,Mirko Pinotti,Alberto Albertini,Mariusz R Wieckowski,Carlotta Giorgi,Roberto Ferrari,Lorenzo Galluzzi,Gianluca Campo,Paolo Pinton

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Aconitase 1/ACO1 抗体 [EPR7226(2)]

Anti-Aconitase 1/ACO1 antibody [EPR7226(2)]

Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (AB183721)

Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (AB183721)

Western blot - Anti-Aconitase 1/ACO1 antibody [EPR7226(2)] (AB183721)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (7)

Abcam product promise