Anti-ACK1 抗体 (ab185726)
Key features and details
- Rabbit polyclonal to ACK1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ACK1 antibody
ACK1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ACK1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Rat, Human -
免疫原
Recombinant full length protein corresponding to Human ACK1 aa 1 to the C-terminus.
Database link: Q07912 -
ポジティブ･コントロール
- SW620, HT-29, MCF7 and Raji cell lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185726の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 115 kDa.
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| IHC-P |
1/50 - 1/200.
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| ICC/IF |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
1/500 - 1/2000. Predicted molecular weight: 115 kDa. |
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IHC-P
1/50 - 1/200. |
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ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Non-receptor tyrosine kinase that regulates the activity of a number of proteins by tyrosine phosphorylation especially proteins critical for cell survival, cell growth, and proliferation. Activates AKT1 by phosphorylating it on 'Tyr-176' resulting in its activation. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' and promotes its recruitment to the androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR. Participates in clathrin-mediated endocytosis. May be involved both in adult synaptic function and plasticity and in brain development. -
組織特異性
The Tyr-284 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. It also shows a significant increase in expression in prostate cancers during the progressive stages. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
Contains 1 SH3 domain.
Contains 1 UBA domain. -
ドメイン
The EBD (EGFR-binding domain) domain is necessary for interaction with EGFR.
The SAM-like domain is necessary for NEDD4-mediated ubiquitination. Promotes membrane localization and dimerization to allow for autophosphorylation.
The UBA domain binds both poly- and mono-ubiquitin. -
翻訳後修飾
Autophosphorylation regulates kinase activity. Phosphorylation on Tyr-518 is required for interaction with SRC.
Ubiquitinated by NEDD4. Its EGF-induced degradation is EGFR activation-dependent and is processed by lysosomes, not proteasomes. -
細胞内局在
Cell membrane. Nucleus. Endosome. Cell junction > adherens junction. Cytoplasmic vesicle membrane. The Tyr-284 phosphorylated form is expressed both in the membrane and nucleus. Co-localizes with EGFR on the endosomes. - Information by UniProt
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参照データベース
- Entrez Gene: 10188 Human
- Entrez Gene: 303882 Rat
- Omim: 606994 Human
- SwissProt: Q07912 Human
- SwissProt: Q5U2X5 Rat
- Unigene: 518513 Human
- Unigene: 98335 Rat
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別名
- Acetate kinase 1 antibody
- Acetokinase 1 antibody
- ACK 1 antibody
see all
画像
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Western blot - Anti-ACK1 antibody (ab185726)All lanes : Anti-ACK1 antibody (ab185726) at 1/500 dilution
Lane 1 : SW620 cell lysate
Lane 2 : HT29 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : Raji cell lysate
Predicted band size: 115 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACK1 antibody (ab185726)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue labelling ACK1 with ab185726 at 1/200. Magnification: 400x. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACK1 antibody (ab185726)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human rectal cancer tissue labelling ACK1 with ab185726 at 1/200. Magnification: 200x. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACK1 antibody (ab185726)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney cancer tissue labelling ACK1 with ab185726 at 1/200. Magnification: 400x. -
Immunocytochemistry/ Immunofluorescence - Anti-ACK1 antibody (ab185726)Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab185726. Blue DAPI for nuclear staining.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab185726 は 2 報の論文で使用されています。
- Peng HH et al. ACK1 upregulated the proliferation of head and neck squamous cell carcinoma cells by promoting p27 phosphorylation and degradation. J Cell Commun Signal 16:567-578 (2022). PubMed: 35247157
- Zhang A et al. TNK2 promoted esophageal cancer progression via activating egfr-akt signaling. J Clin Lab Anal 35:e23700 (2021). PubMed: 33484472