Anti-Acid phosphatase/ACP1 抗体 [EPR9839] (ab166896)
![Western blot - Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896)](https://www.abcam.co.jp/ps/products/166/ab166896/Images/ab166896-349166-antiacid-phosphatase-antibody-epr9839-western-blot-knock-out-hek293-k562-hela-empty-human-lysates.jpg "Western blot - Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9839] to Acid phosphatase/ACP1
- Suitable for: Flow Cyt (Intra), WB, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Acid phosphatase/ACP1 antibody [EPR9839]
Acid phosphatase/ACP1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR9839] to Acid phosphatase/ACP1 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IPmore details
適用なし: ICC/IF or IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human placenta, HeLa and HepG2 lysates; permeabilized Hela cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR9839 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab166896の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
|
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| IP |
1/10 - 1/100.
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 18 kDa. |
|
IP
1/10 - 1/100. |
ターゲット情報
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機能
Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Isoform 3 does not possess phosphatase activity. -
組織特異性
T-lymphocytes express only isoform 2. -
配列類似性
Belongs to the low molecular weight phosphotyrosine protein phosphatase family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 52 Human
- Entrez Gene: 11431 Mouse
- Entrez Gene: 631286 Mouse
- Entrez Gene: 24161 Rat
- Omim: 171500 Human
- SwissProt: P24666 Human
- SwissProt: Q9D358 Mouse
- SwissProt: P41498 Rat
see all -
別名
- Acid phosphatase 1 soluble antibody
- Acid phosphatase of erythrocyte antibody
- ACP1 antibody
see all
画像
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Western blot - Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896)All lanes : Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896) at 1/1000 dilution
Lane 1 : Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : ACP1 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 18 kDaLanes 1 - 4: Merged signal (red and green). Green - ab166896 observed at 18 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab166896 was shown to recognize ACP1 (Acid phosphatase 1) in wild-type HEK-293 cells as signal was lost at the expected MW in ACP1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ACP1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab166896 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896)](https://www.abcam.co.jp/ps/products/166/ab166896/Images/ab166896-165245-anti-acid-phosphatase-antibody-epr9839-western-blot.jpg)
Western blot - Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896)All lanes : Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896) at 1/1000 dilution
Lane 1 : Human placenta lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP antibody at 1/2000 dilution
Predicted band size: 18 kDa -
![Flow Cytometry (Intracellular) - Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896)](https://www.abcam.co.jp/ps/products/166/ab166896/Images/ab166896-165244-anti-acid-phosphatase-antibody-epr9839-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896)Intracellular flow cytometric analysis of permeabilized HeLa cells labeling Acid Phosphatase/ACP1 using ab166896 at 1/10 dilution (red) compared to staining with a rabbi IgG negative control antibody (green).
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![Immunoprecipitation - Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896)](https://www.abcam.co.jp/ps/products/166/ab166896/Images/ab166896-165243-anti-acid-phosphatase-antibody-epr9839-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896)Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab166896 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG, preferentially detecting the non-reduced form of rabbit IgG. -
![Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896)](https://www.abcam.co.jp/ps/products/166/ab166896/Images/ab166896-5-benefits-of-recombinant-antibodies.png)
Anti-Acid phosphatase/ACP1 antibody [EPR9839] (ab166896)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab166896 は 2 報の論文で使用されています。
- Locard-Paulet M et al. Phosphoproteomic analysis of interacting tumor and endothelial cells identifies regulatory mechanisms of transendothelial migration. Sci Signal 9:ra15 (2016). Human . PubMed: 26861043
- Llewellyn KJ et al. A Fine Balance of Dietary Lipids Improves Pathology of a Murine Model of VCP-Associated Multisystem Proteinopathy. PLoS One 10:e0131995 (2015). WB ; Mouse . PubMed: 26134519