Anti-Acetylcholinesterase 抗体 (ab31276)
Key features and details
- Goat polyclonal to Acetylcholinesterase
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Acetylcholinesterase antibody
Acetylcholinesterase 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Acetylcholinesterase -
由来種
Goat -
特異性
This antibody is expected to recognise isoform NP_000656 only (the ubiquitously expressed, hydrophillic form). -
アプリケーション
適用あり: WB, ICC/IF, Flow Cytmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Rabbit, Cow, Cat
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免疫原
Synthetic peptide:
QFDHYSKQDRCSDL
, corresponding to C terminal amino acids 601-614 of Human Acetylcholinesterase -
ポジティブ･コントロール
- WB: Jurkat, HepG2, SkHep1, Snu387, and SW620 cell lysates. Flow Cyt: HeLa cells ICC/IF: U2OS cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31276の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (3) |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
1 hour primary incubation is recommended for this product. |
| ICC/IF |
Use a concentration of 10 µg/ml.
|
|
| Flow Cyt |
Use a concentration of 10 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa). 1 hour primary incubation is recommended for this product. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
|
Flow Cyt
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. -
組織特異性
Isoform H is highly expressed in erythrocytes. -
配列類似性
Belongs to the type-B carboxylesterase/lipase family. -
細胞内局在
Cell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei. - Information by UniProt
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参照データベース
- Entrez Gene: 540446 Cow
- Entrez Gene: 43 Human
- Entrez Gene: 83817 Rat
- Omim: 100740 Human
- SwissProt: P23795 Cow
- SwissProt: P22303 Human
- SwissProt: Q29499 Rabbit
- SwissProt: P37136 Rat
see all -
別名
- ACEE antibody
- ACES_HUMAN antibody
- Acetylcholinesterase antibody
see all
画像
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Flow Cytometry - Anti-Acetylcholinesterase antibody (ab31276)Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Western blot - Anti-Acetylcholinesterase antibody (ab31276)All lanes : Anti-Acetylcholinesterase antibody (ab31276) at 0.3 µg/ml
Lane 1 : Jurkat cell lysate at 0.5 µg/ml
Lane 2 : HepG2 cell lysate in RIPA buffer at 35 µg
Predicted band size: 68 kDaDetected by chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-Acetylcholinesterase antibody (ab31276)Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing nuclear, membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL). -
Western blot - Anti-Acetylcholinesterase antibody (ab31276)This image is courtesy of an Abreview submitted by Ender AvciAll lanes : Anti-Acetylcholinesterase antibody (ab31276) at 1/200 dilution
Lane 1 : SkHep1 cell lysate
Lane 2 : Snu387 cell lysate
Lane 3 : SW620 cell lysate
Lane 4 : Zebrafish brain lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated Rabbit anti-goat IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Exposure time: 20 minutes
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab31276 は 14 報の論文で使用されています。
- Ruivo CF et al. Extracellular Vesicles from Pancreatic Cancer Stem Cells Lead an Intratumor Communication Network (EVNet) to fuel tumour progression. Gut N/A:N/A (2022). PubMed: 35012996
- Martin-Jaular L et al. Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV-1 infection. EMBO J 40:e105492 (2021). PubMed: 33709510
- Atali S et al. Interaction of amyloid beta with humanin and acetylcholinesterase is modulated by ATP. FEBS Open Bio 10:2805-2823 (2020). PubMed: 33145964
- Cocozza F et al. Extracellular vesicles containing ACE2 efficiently prevent infection by SARS-CoV-2 Spike protein-containing virus. J Extracell Vesicles 10:e12050 (2020). PubMed: 33391636
- Yekta R et al. The inefficacy of donepezil on glycated-AChE inhibition: Binding affinity, complex stability and mechanism. Int J Biol Macromol 160:35-46 (2020). PubMed: 32454110