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AB244777

Anti-ACE2 抗体 [EPR20239-267] - BSA and Azide free (Capture)

Anti-ACE2 antibody [EPR20239-267] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal ACE2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

別名を表示する

UNQ868/PRO1885, ACE2, Angiotensin-converting enzyme 2, Angiotensin-converting enzyme homolog, Angiotensin-converting enzyme-related carboxypeptidase, Metalloprotease MPROT15, ACEH, ACE-related carboxypeptidase

1 Images
Sandwich ELISA - Anti-ACE2 antibody [EPR20239-267] - BSA and Azide free (Capture) (AB244777)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-ACE2 antibody [EPR20239-267] - BSA and Azide free (Capture) (AB244777)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab235649).

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR20239-267

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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組合せ製品

Assay Kits

AB273297

Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric)

Functional Studies - Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric) (AB273297)

  • 3
  • 2
Primary Antibodies

AB109131

Anti-TMPRSS2 antibody [EPR3862]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS2 antibody [EPR3862] (AB109131)

  • 5
  • 3
Primary Antibodies

AB283946

Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174]

20ul selling size|RabMAb|Recombinant

Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] (AB283946)

  • 0
  • 1
Proteins & Peptides

AB276202

Recombinant human ACE2 protein (His tag)

Functional Studies - Recombinant human ACE2 protein (His tag) (AB276202)

  • 0
  • 0
Primary Antibodies

AB254222

Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Multiplex immunohistochemistry - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (AB254222)

  • 5
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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製品の詳細

ab244777 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human ACE2. The recommended pair for sandwich ELISA is:
Capture: ab244777, Human ACE2 Capture Antibody (unconjugated)
Detector: ab245050, Human ACE2 Detector Antibody (unconjugated)
The reference range value is 3.98 - 255 ng/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The ACE2 protein also known as angiotensin-converting enzyme 2 is an essential component in the renin-angiotensin system. It functions mechanically by converting the hormone angiotensin II to angiotensin-(1-7) which helps regulate blood pressure and fluid balance. The molecular weight of ACE2 is approximately 120 kDa. This protein is expressed in various tissues particularly the lungs heart kidneys and gastrointestinal tract. In cultured cells like Caco-2 cells researchers often study this expression.
Biological function summary

The ACE2 protein plays an important role in the regulation of cardiovascular and renal functions. It is a single-pass type I membrane protein and its activity reduces inflammation and oxidative stress in cells. ACE2 does not function as part of a larger protein complex but its enzymatic conversion has a substantial impact on reducing the effects of angiotensin II in the body leading to vasodilation and decreased blood pressure.

Pathways

ACE2 involvement is significant in the renin-angiotensin system and the kallikrein-kinin system. These pathways are essential for maintaining cardiovascular homeostasis. In the renin-angiotensin system ACE2 works in opposition to angiotensin-converting enzyme (ACE) balancing the effects through the production of angiotensin-(1-7) from angiotensin II. Additionally ACE2 interacts indirectly with proteins like angiotensin receptor type 1 (AT1) and angiotensin receptor type 2 (AT2) ensuring proper signaling and physiological responses.

ACE2 links closely with conditions such as hypertension and COVID-19. Increased activity of angiotensin II due to low ACE2 levels contributes to hypertension. In infectious disease SARS-CoV-2 virus responsible for COVID-19 uses ACE2 as an entry receptor to initiate infection in host cells. This interaction highlights the importance of ACE2 in disease pathogenesis and has prompted interest in ACE2 as a potential therapeutic target.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis (PubMed : 27217402). Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II (PubMed : 10924499, PubMed : 10969042, PubMed : 11815627, PubMed : 14504186, PubMed : 19021774). Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin (PubMed : 10969042, PubMed : 11815627). Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency (PubMed : 11815627, PubMed : 27217402, PubMed : 28293165). In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity (PubMed : 18424768, PubMed : 19185582).. (Microbial infection) Acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63.. Isoform 2. Non-functional as a carboxypeptidase.. Isoform 2. (Microbial infection) Non-functional as a receptor for human coronavirus SARS-CoV-2.
See full target information ACE2
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