Name: Anti-ACAT1 antibody [EPR10359]
Brand: RabMAb
SKU: ab168342
Rating: 5 (3 reviews)

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Western blot - Anti-ACAT1 antibody [EPR10359] (ab168342)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10359] to ACAT1
Suitable for: WB, IP, IHC-P
Reacts with: Human

Carrier Free

製品名

Anti-ACAT1 antibody [EPR10359]
ACAT1 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR10359] to ACAT1
由来種

Rabbit
アプリケーション

適用あり: WB, IP, IHC-Pmore details
適用なし: Flow Cyt or ICC/IF
種交差性

交差種: Human
交差が予測される動物種: Mouse, Rat
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- Human fetal liver, SW480, Jurkat, HepG2, (C) SKBR-3, THP-1 and Human fetal kidney lysates, Human heart & liver tissue, immunoprecipitation pellet from Human fetal liver lysate
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
バッファー

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR10359
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-ACAT1 antibody [EPR10359] - BSA and Azide free (ab240152)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human ACAT1 knockout SW480 cell line (ab269621)
KO cell lysates
- Human ACAT1 knockout SW480 cell lysate (ab272431)
Positive Controls
- Hep G2 whole cell lysate (ab166833)
- THP-1 whole cell lysate (ab7913)
Recombinant Protein
- Recombinant Human ACAT1 protein (ab137175)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab168342の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB	(1)	1/1000 - 1/5000. Predicted molecular weight: 45 kDa.
IP		1/10 - 1/100.
IHC-P	(1)	1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

特記事項
WB 1/1000 - 1/5000. Predicted molecular weight: 45 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

追加情報

Is unsuitable for Flow Cyt or ICC/IF.

機能

Plays a major role in ketone body metabolism.
関連疾患

Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD) [MIM:203750]; also known as alpha-methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype.
配列類似性

Belongs to the thiolase family.
細胞内局在

Mitochondrion.
Target information above from: UniProt accession P24752 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 38 Human
- Entrez Gene: 110446 Mouse
- Entrez Gene: 25014 Rat
- Omim: 607809 Human
- SwissProt: P24752 Human
- SwissProt: Q8QZT1 Mouse
- SwissProt: P17764 Rat
- Unigene: 232375 Human
- Unigene: 293233 Mouse
- Unigene: 4054 Rat
see all
別名
- ACAT 1 antibody
- ACAT antibody
- acat1 antibody
- Acetoacetyl CoA thiolase antibody
- acetoacetyl Coenzyme A thiolase antibody
- Acetoacetyl-CoA thiolase antibody
- Acetyl CoA acetyltransferase, mitochondrial antibody
- Acetyl Coenzyme A acetyltransferase 1 antibody
- Acetyl-CoA acetyltransferase antibody
- acetyl-coa acetyltransferase precursor, mitochondrial antibody
- Acetyl-CoA thiolase, mitochondrial antibody
- acetyl-Coenzyme A acetyltransferase 1 antibody
- MAT antibody
- mitochondrial acetoacetyl-CoA thiolase antibody
- mitochondrial antibody
- RATACAL antibody
- T2 antibody
- testicular tissue protein Li 198 antibody
- THIL antibody
- THIL_HUMAN antibody
see all

Western blot - Anti-ACAT1 antibody [EPR10359] (ab168342)

All lanes : Anti-ACAT1 antibody [EPR10359] (ab168342) at 1/1000 dilution

Lane 1 : Wild-type SW480 cell lysate
Lane 2 : ACAT1 CRISPR/Cas9 edited SW480 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 45 kDa
Observed band size: 45 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab168342 observed at 45 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab168342 was shown to react with ACAT1 in western blot. The band observed in the CRISPR/Cas9 edited lysate lane below 45 kDa is likely to represent a truncated form. This has not been investigated further. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab168342 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody [EPR10359] (ab168342)

ab168342 showing +ve staining in Human ovarian carcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-ACAT1 antibody [EPR10359] (ab168342)

All lanes : Anti-ACAT1 antibody [EPR10359] (ab168342) at 1/1000 dilution

Lane 1 : Human fetal liver lysates
Lane 2 : HepG2 lysates
Lane 3 : SKBR-3 lysates
Lane 4 : THP-1 lysates
Lane 5 : Human fetal kidney lysates

Lysates/proteins at 10 µg per lane.

Predicted band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody [EPR10359] (ab168342)

ab168342 showing +ve staining in Human normal kidney tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody [EPR10359] (ab168342)

ab168342 showing +ve staining in Human normal colon tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody [EPR10359] (ab168342)

ab168342 showing +ve staining in Human papillary adenocarcinoma of thyroid gland tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody [EPR10359] (ab168342)

Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling ACAT1 with ab168342 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody [EPR10359] (ab168342)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ACAT1 with ab168342 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunoprecipitation - Anti-ACAT1 antibody [EPR10359] (ab168342)

Western blot analysis labelling ACAT1 on immunoprecipitation pellet from Human fetal liver lysate using ab168342 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Anti-ACAT1 antibody [EPR10359] (ab168342)

Western blot protocols
Immunoprecipitation protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab168342 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab168342 は 16 報の論文で使用されています。

Vergani E et al. Targeting of the Lipid Metabolism Impairs Resistance to BRAF Kinase Inhibitor in Melanoma. Front Cell Dev Biol 10:927118 (2022). PubMed: 35912092
Wang Y et al. 27-Hydroxycholesterol-Induced Dysregulation of Cholesterol Metabolism Impairs Learning and Memory Ability in ApoE ε4 Transgenic Mice. Int J Mol Sci 23:N/A (2022). PubMed: 36232940
Zhang Z et al. HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARa axis. Mol Ther Nucleic Acids 24:711-727 (2021). PubMed: 33996254
Thai PN et al. Ketone Ester D-β-Hydroxybutyrate-(R)-1,3 Butanediol Prevents Decline in Cardiac Function in Type 2 Diabetic Mice. J Am Heart Assoc 10:e020729 (2021). PubMed: 34583524
Chanyshev MD et al. Downregulation of Acat1 by miR-21 may participate in liver fibrosis upon chronic DDT exposure. Toxicol Mech Methods N/A:1-8 (2020). PubMed: 32508177

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-3 of 3 Abreviews or Q&A

Application

Western blot

Sample

Rat Tissue lysate - whole (Heart tissue)

Gel Running Conditions

Reduced Denaturing (4-12%)

Loading amount

7.5 µg

Specification

Heart tissue

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MISS. Kaitlyn Dennis

Verified customer

投稿 Jan 31 2022

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Kidney)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0

Permeabilization

Yes - 0.05% Tween 20

Specification

Kidney

Blocking step

Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 20 2020

Application

Immunohistochemistry (Frozen sections)

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Sample

Mouse Cell (Retina: GCL,OPL, POS)

Specification

Retina: GCL,OPL, POS

Permeabilization

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 24 2015

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

KO cell lines

KO cell lysates

Positive Controls

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

関連疾患

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (16)

レビューと Q&A

Western blot abreview for Anti-ACAT1 antibody [EPR10359]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ACAT1 antibody [EPR10359]

Immunohistochemistry (Frozen sections) abreview for Anti-ACAT1 antibody [EPR10359]