Anti-ACAT1 抗体 (ab154396)
Key features and details
- Rabbit polyclonal to ACAT1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ACAT1 antibody
ACAT1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ACAT1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Cow
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免疫原
Recombinant fragment corresponding to Human ACAT1 aa 1-151.
Database link: P24752 -
ポジティブ･コントロール
- WB: SW480, HepG2, Jurkat, HEK-293T, A431 and Molt-4 whole cell lysate. Mouse brain whole cell lysate. ICC/IF: HeLa and A431 cells. IHC-P: Human normal kidney tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154396の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 45 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| IHC-P |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 45 kDa. |
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ICC/IF
1/100 - 1/1000. |
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IHC-P
1/100 - 1/1000. |
ターゲット情報
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機能
Plays a major role in ketone body metabolism. -
関連疾患
Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD) [MIM:203750]; also known as alpha-methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype. -
配列類似性
Belongs to the thiolase family. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 511082 Cow
- Entrez Gene: 38 Human
- Entrez Gene: 110446 Mouse
- Omim: 607809 Human
- SwissProt: Q29RZ0 Cow
- SwissProt: P24752 Human
- SwissProt: Q8QZT1 Mouse
- Unigene: 232375 Human
see all -
別名
- ACAT 1 antibody
- ACAT antibody
- acat1 antibody
see all
画像
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Western blot - Anti-ACAT1 antibody (ab154396)All lanes : Anti-ACAT1 antibody (ab154396) at 1/500 dilution
Lane 1 : Wild-type SW480 cell lysate
Lane 2 : ACAT1 CRISPR/Cas9 edited SW480 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 45 kDaLanes 1 - 4: Merged signal (red and green). Green - ab154396 observed at 45 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab154396 was shown to react with ACAT1 in western blot. The band observed in the knockout cell line ab269621 (knockout cell lysate ab272431) lane below 45 kDa is likely to represent a truncated form. This has not been investigated further. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab154396 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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Immunocytochemistry/ Immunofluorescence - Anti-ACAT1 antibody (ab154396)A431 cells stained for ACAT1 using ab154396 at 1/1000 dilution in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAT1 antibody (ab154396)Paraffin embedded human normal kidney tissue stained for ACAT1 using ab154396 at 1/1000 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-ACAT1 antibody (ab154396)HeLa cells stained for ACAT1 using ab154396 at 1/1000 dilution in ICC/IF.
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Western blot - Anti-ACAT1 antibody (ab154396)All lanes : Anti-ACAT1 antibody (ab154396) at 1/500 dilution
Lane 1 : HEK-293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : Molt-4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
10% SDS PAGE -
Western blot - Anti-ACAT1 antibody (ab154396)Anti-ACAT1 antibody (ab154396) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 45 kDa
10% SDS PAGE
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab154396 は 1 報の論文で使用されています。
- Liu Z et al. Correlation of cholesteryl ester metabolism to pathogenesis, progression and disparities in colorectal Cancer. Lipids Health Dis 21:22 (2022). PubMed: 35172832