AB155138

Anti-ACADVL/VLCAD 抗体

Name: Anti-ACADVL/VLCAD 抗体 (ab155138) | Abcam
Brand: Abcam Limited
SKU: AB155138
Price: 75000 JPY
Availability: InStock
Rating: 4 (2 reviews)

Anti-ACADVL/VLCAD antibody

(2 Reviews)

(13 Publications)

Rabbit Polyclonal ACADVL/VLCAD antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACADVL aa 1-350.

別名を表示する

VLCAD, ACADVL

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody (AB155138)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody (AB155138)

Immunohistochemical analysis of paraffin embedded H358 xenograft labeling ACADVL/VLCAD with ab155138 antibody at 1/250.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody (AB155138)

Immunohistochemical analysis of paraffin-embedded human a549 xenograft tissue stained for ACADVL protein at mitochondria with ab155138 at a 1/500 dilution.

Antigen retrieval : EDTA based, pH 8.0 buffer, 15min

Immunocytochemistry/ Immunofluorescence - Anti-ACADVL/VLCAD antibody (AB155138)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ACADVL/VLCAD antibody (AB155138)

Immunofluorescence analysis of HeLa cells fixed in ice-cold MeOH for 5 min labelling ACADVL protein at mitochondria using ab155138 at a 1/500 dilution (Green). DAPI was used for nuclear staining (Blue).
Scale bar = 10 µM

Unknown

Western blot - Anti-ACADVL/VLCAD antibody (AB155138)

7.5% SDS PAGE

All lanes:

Western blot - Anti-ACADVL/VLCAD antibody (ab155138) at 1/3000 dilution

All lanes:

Mouse liver whole cell lysate at 50 µg

Predicted band size: 70 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

WB, IHC-P, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human ACADVL aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

P49748

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ACADVL also known as VLCAD (Very Long-Chain Acyl-CoA Dehydrogenase) is an enzyme that plays an important role in the breakdown of long-chain fatty acids within the mitochondria. It has a molecular mass of approximately 70 kDa. VLCAD is expressed in various tissues with high levels found in the heart skeletal muscle and liver. These tissues are heavily reliant on fatty acid oxidation for energy production especially when glucose availability is low.

Biological function summary

ACADVL functions within the mitochondrial matrix where it catalyzes the initial step of beta-oxidation by desaturating long-chain acyl-CoA molecules. This enzyme is a part of a larger complex that includes other mitochondrial dehydrogenases contributing to the degradation of fatty acids into acetyl-CoA units. The process is essential for energy production especially under fasting conditions or high-energy demand scenarios such as muscle contraction and cardiac function.

Pathways

VLCAD plays an essential role in the fatty acid beta-oxidation pathway. It works alongside other enzymes such as ACADM (Medium-Chain Acyl-CoA Dehydrogenase) to facilitate the sequential shortening of fatty acids which integrates into the Krebs cycle for further energy extraction. This pathway maximizes energy yield from fatty acids significantly influencing cellular metabolism and energy homeostasis.

VLCAD deficiencies lead to severe metabolic conditions such as VLCAD deficiency. This disorder results in the impaired breakdown of long-chain fatty acids causing energy deficiency and the accumulation of toxic substances. Symptoms range from muscle weakness to serious cardiac complications. VLCAD deficiency's impact is often assessed in relation to proteins like ACSL1 and CPT2 which are involved in the fatty acid metabolism pathway and are central to effective energy processing in cells.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Very long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation (FAO), breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (PubMed : 17564966, PubMed : 18227065, PubMed : 7668252, PubMed : 9461620, PubMed : 9599005, PubMed : 9839948). The first step of FAO consists in the proR-proR stereospecific alpha, beta-dehydrogenation of fatty acyl-CoA thioesters using the electron transfer flavoprotein (ETF) as their physiologic electron acceptor, resulting in the formation of trans-2-enoyl-CoA ((2E)-enoyl-CoA) (PubMed : 18227065, PubMed : 7668252, PubMed : 9461620, PubMed : 9839948). Among the different mitochondrial acyl-CoA dehydrogenases, very long-chain specific acyl-CoA dehydrogenase acts specifically on fatty acyl-CoAs with saturated 12 to 24 carbons long primary chains (PubMed : 17564966, PubMed : 21237683, PubMed : 9839948).

See full target information ACADVL

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文献 (13)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 12:14576 PubMed36028747

2022

PERM1 regulates genes involved in fatty acid metabolism in the heart by interacting with PPARα and PGC-1α.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Yang Huang,Shin-Ichi Oka,Xiaoyong Xu,Chian-Feng Chen,Chien-Yi Tung,Ya-Yuan Chang,Youssef Mourad,Omair Vehra,Andreas Ivessa,Ghassan Yehia,Peter Romanienko,Chiao-Po Hsu,Junichi Sadoshima

Cellular and molecular life sciences : CMLS 79:375 PubMed35727412

2022

Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Zhi Peng,Yong-Xian Shao,Xiu-Zhen Li,Kang-Di Zhang,Yan-Na Cai,Yun-Ting Lin,Min-Yan Jiang,Zong-Cai Liu,Xue-Ying Su,Wen Zhang,Xiao-Ling Jiang,Li Liu

Autophagy 18:1367-1384 PubMed34689722

2021

Muscle-generated BDNF (brain derived neurotrophic factor) maintains mitochondrial quality control in female mice.

Applications

Unspecified application

Species

Unspecified reactive species

Palak Ahuja,Chun Fai Ng,Brian Pak Shing Pang,Wing Suen Chan,Margaret Chui Ling Tse,Xinyi Bi,Hiu-Lam Rachel Kwan,Daniel Brobst,Oana Herlea-Pana,Xiuying Yang,Guanhua Du,Suchaorn Saengnipanthkul,Hye Lim Noh,Baowei Jiao,Jason K Kim,Chi Wai Lee,Keqiang Ye,Chi Bun Chan

Journal of Zhejiang University. Science. B 21:885-896 PubMed33150772

2020

Novel ACADVL variants resulting in mitochondrial defects in long-chain acyl-CoA dehydrogenase deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Chen,Fan Tong,Xiao-Yu Wu,Ling Zhu,Qiu-Zi Yi,Jing Zheng,Ru-Lai Yang,Zheng-Yan Zhao,Xiao-Hui Cang,Qiang Shu,Ping-Ping Jiang

Theranostics 10:8197-8210 PubMed32724466

2020

Brown adipose tissue-derived exosomes mitigate the metabolic syndrome in high fat diet mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xueying Zhou,Zhelong Li,Meihao Qi,Ping Zhao,Yunyou Duan,Guodong Yang,Lijun Yuan

Molecular metabolism 37:100981 PubMed32283081

2020

Mitochondrial disease disrupts hepatic allostasis and lowers the threshold for immune-mediated liver toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Maxim Jestin,Senta M Kapnick,Tatyana N Tarasenko,Cassidy T Burke,Patricia M Zerfas,Francisca Diaz,Hilary Vernon,Larry N Singh,Ronald J Sokol,Peter J McGuire

American journal of physiology. Endocrinology and metabolism 318:E145-E151 PubMed31794263

2019

Combined overexpression of SIRT1 and knockout of GCN5 in adult skeletal muscle does not affect glucose homeostasis or exercise performance in mice.

Applications

Species

Mouse

Kristoffer Svensson,Shahriar Tahvilian,Vitor F Martins,Jessica R Dent,Adrianna Lemanek,Neeka Barooni,Keenan Greyslak,Carrie E McCurdy,Simon Schenk

Oxidative medicine and cellular longevity 2019:9894238 PubMed31737181

2019

Kynurenine, a Tryptophan Metabolite That Increases with Age, Induces Muscle Atrophy and Lipid Peroxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Helen Kaiser,Kanglun Yu,Chirayu Pandya,Bharati Mendhe,Carlos M Isales,Meghan E McGee-Lawrence,Maribeth Johnson,Sadanand Fulzele,Mark W Hamrick

Bioscience reports 39: PubMed30867255

2019

Distinct metabolic patterns during microglial remodeling by oleate and palmitate.

Applications

Unspecified application

Species

Unspecified reactive species

Bruno Chausse,Pamela A Kakimoto,Camille C Caldeira-da-Silva,Adriano B Chaves-Filho,Marcos Y Yoshinaga,Railmara Pereira da Silva,Sayuri Miyamoto,Alicia J Kowaltowski

PloS one 14:e0211733 PubMed30716103

2019

Resilient hepatic mitochondrial function and lack of iNOS dependence in diet-induced insulin resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Pamela A Kakimoto,Bruno Chausse,Camille C Caldeira da Silva,José Donato Júnior,Alicia J Kowaltowski

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACADVL/VLCAD 抗体

Anti-ACADVL/VLCAD antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody (AB155138)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADVL/VLCAD antibody (AB155138)

Immunocytochemistry/ Immunofluorescence - Anti-ACADVL/VLCAD antibody (AB155138)

Western blot - Anti-ACADVL/VLCAD antibody (AB155138)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (13)

Abcam product promise