Anti-ACADS/SCAD 抗体 [EPR10861(B)] (ab154823)
![Western blot - Anti-ACADS/SCAD antibody [EPR10861(B)] (ab154823)](https://www.abcam.co.jp/ps/products/154/ab154823/Images/ACADS-Primary-antibodies-ab154823-1.JPG "Western blot - Anti-ACADS/SCAD antibody [EPR10861(B)] (ab154823)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10861(B)] to ACADS/SCAD
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ACADS/SCAD antibody [EPR10861(B)]
ACADS/SCAD 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10861(B)] to ACADS/SCAD -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- HeLa; HepG2 and 293T cell lysates; Human fetal heart and liver tissue lysates; 293T cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR10861(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154823の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
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| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 44 kDa. |
追加情報
Is unsuitable for ICC/IF,IHC-P or IP.
ターゲット情報
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パスウェイ
Lipid metabolism; mitochondrial fatty acid beta-oxidation. -
関連疾患
Defects in ACADS are the cause of acyl-CoA dehydrogenase short-chain deficiency (ACADSD) [MIM:201470]. It is an autosomal recessive disorder resulting in acute acidosis and muscle weakness in infants, and a form of lipid-storage myopathy in adults. -
配列類似性
Belongs to the acyl-CoA dehydrogenase family. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 35 Human
- Entrez Gene: 11409 Mouse
- Entrez Gene: 64304 Rat
- Omim: 606885 Human
- SwissProt: P16219 Human
- SwissProt: Q07417 Mouse
- SwissProt: P15651 Rat
- Unigene: 507076 Human
see all -
別名
- ACAD3 antibody
- ACADS antibody
- ACADS_HUMAN antibody
see all
画像
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Western blot - Anti-ACADS/SCAD antibody [EPR10861(B)] (ab154823)All lanes : Anti-ACADS/SCAD antibody [EPR10861(B)] (ab154823) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Human fetal heart tissue lysate
Lane 5 : Human fetal liver tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 44 kDa -
![Flow Cytometry (Intracellular) - Anti-ACADS/SCAD antibody [EPR10861(B)] (ab154823)](https://www.abcam.co.jp/ps/products/154/ab154823/Images/ACADS-Primary-antibodies-ab154823-2.JPG)
Flow Cytometry (Intracellular) - Anti-ACADS/SCAD antibody [EPR10861(B)] (ab154823)Intracellular flow cytometric analysis of permeabilized 293T cells labeling ACADS/SCAD using ab154823 (red) at 1/10 dilution, or rabbit IgG (negative) (green).
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![Anti-ACADS/SCAD antibody [EPR10861(B)] (ab154823)](https://www.abcam.co.jp/ps/products/154/ab154823/Images/ab154823-3-benefits-of-recombinant-antibodies.png)
Anti-ACADS/SCAD antibody [EPR10861(B)] (ab154823)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab154823 は 8 報の論文で使用されています。
- Park B et al. Pyruvate kinase M1 regulates butyrate metabolism in cancerous colonocytes. Sci Rep 12:8771 (2022). PubMed: 35610475
- Zhang B et al. Effects of riboflavin deficiency on the lipid metabolism of duck breeders and duck embryos. Poult Sci 100:101342 (2021). PubMed: 34438327
- Broniarek I et al. The Influence of Statins on the Aerobic Metabolism of Endothelial Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32098258
- Escudero S et al. Dynamic Regulation of Long-Chain Fatty Acid Oxidation by a Noncanonical Interaction between the MCL-1 BH3 Helix and VLCAD. Mol Cell 69:729-743.e7 (2018). PubMed: 29499131
- Koziel A & Jarmuszkiewicz W Hypoxia and aerobic metabolism adaptations of human endothelial cells. Pflugers Arch 469:815-827 (2017). PubMed: 28176017
- Tang J et al. Severe riboflavin deficiency induces alterations in the hepatic proteome of starter Pekin ducks. Br J Nutr 118:641-650 (2017). PubMed: 29185933
- Broniarek I et al. The effect of chronic exposure to high palmitic acid concentrations on the aerobic metabolism of human endothelial EA.hy926 cells. Pflugers Arch 468:1541-54 (2016). WB ; Human . PubMed: 27417103
- Meierhofer D et al. Ataxin-2 (Atxn2)-Knock-Out Mice Show Branched Chain Amino Acids and Fatty Acids Pathway Alterations. Mol Cell Proteomics 15:1728-39 (2016). PubMed: 26850065