Anti-ACADM/MCAD 抗体 (ab13677)
Key features and details
- Goat polyclonal to ACADM/MCAD
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ACADM/MCAD antibody
ACADM/MCAD 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to ACADM/MCAD -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human ACADM/MCAD aa 400 to the C-terminus (C terminal).
Database link: P11310 -
ポジティブ・コントロール
- Human Heart Lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13677の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
Use a concentration of 0.01 - 0.1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).
A 1 hour primary incubation is recommended for this product. |
IHC-P |
Use a concentration of 3.75 µg/ml.
|
特記事項 |
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WB
Use a concentration of 0.01 - 0.1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa). A 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 3.75 µg/ml. |
ターゲット情報
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機能
This enzyme is specific for acyl chain lengths of 4 to 16. -
パスウェイ
Lipid metabolism; mitochondrial fatty acid beta-oxidation. -
関連疾患
Defects in ACADM are the cause of acyl-CoA dehydrogenase medium-chain deficiency (ACADMD) [MIM:201450]. It is an autosomal recessive disease which causes fasting hypoglycemia, hepatic dysfunction, and encephalopathy, often resulting in death in infancy. -
配列類似性
Belongs to the acyl-CoA dehydrogenase family. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 34 Human
- Omim: 607008 Human
- SwissProt: P11310 Human
- Unigene: 445040 Human
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別名
- ACAD 1 antibody
- ACAD1 antibody
- Acadm antibody
see all
画像
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Anti-ACADM/MCAD antibody (ab13677) at 0.05 µg/ml + Human heart lysates at 35 µg
Developed using the ECL technique.
Predicted band size: 48 kDaAb13677 staining (0.05
µ g/ml) of Human Heart lysate (RIPA buffer, 35µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Primary incubated for 1 hour.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab13677 は 2 報の論文で使用されています。
- Wang F et al. PP56 improves energy homeostasis in a mouse model of pancreatic cancer. Tumour Biol 31:309-13 (2010). WB ; Mouse . PubMed: 20422342
- Powelka AM et al. Suppression of oxidative metabolism and mitochondrial biogenesis by the transcriptional corepressor RIP140 in mouse adipocytes. J Clin Invest 116:125-36 (2006). WB ; Mouse . PubMed: 16374519