Anti-ACADL/LCAD 抗体 (ab152160)
Key features and details
- Rabbit polyclonal to ACADL/LCAD
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ACADL/LCAD antibody
ACADL/LCAD 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ACADL/LCAD -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Pig
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免疫原
Recombinant fragment corresponding to Human ACADL/LCAD aa 1-134 (N terminal).
Database link: P28330 -
ポジティブ･コントロール
- Recombinant Human ACADL/LCAD protein (ab114591) can be used as a positive control in WB. Human hepatoma tissue; H1299 and HCT116 whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab152160の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 48 kDa.
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| IHC-P |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 48 kDa. |
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IHC-P
1/100 - 1/1000. |
ターゲット情報
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パスウェイ
Lipid metabolism; mitochondrial fatty acid beta-oxidation. -
関連疾患
Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD) [MIM:201475]. An inborn error of mitochondrial fatty acid beta-oxidation which leads to impaired long-chain fatty acid beta-oxidation. It is clinically heterogeneous, with three major phenotypes: a severe childhood form characterized by early onset, high mortality and high incidence of cardiomyopathy; a milder childhood form with later onset, characterized by hypoketotic hypoglycemia, low mortality and rare cardiomyopathy; an adult form, with isolated skeletal muscle involvement, rhabdomyolysis and myoglobinuria, usually triggered by exercise or fasting. -
配列類似性
Belongs to the acyl-CoA dehydrogenase family. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 33 Human
- Entrez Gene: 396931 Pig
- Omim: 609576 Human
- SwissProt: P28330 Human
- SwissProt: P79274 Pig
- Unigene: 471277 Human
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別名
- ACAD4 antibody
- ACADL antibody
- ACADL_HUMAN antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADL/LCAD antibody (ab152160)
Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue, staining ACADL/LCAD using ab152160 at a 1/500 dilution.
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Western blot - Anti-ACADL/LCAD antibody (ab152160)All lanes : Anti-ACADL/LCAD antibody (ab152160) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HCT116 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 48 kDa
10% SDS PAGE
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab152160 は 2 報の論文で使用されています。
- Pouliquen DL et al. Long-Chain Acyl Coenzyme A Dehydrogenase, a Key Player in Metabolic Rewiring/Invasiveness in Experimental Tumors and Human Mesothelioma Cell Lines. Cancers (Basel) 15:N/A (2023). PubMed: 37297007
- Huang YW et al. Dysregulated Free Fatty Acid Receptor 2 Exacerbates Colonic Adenoma Formation in ApcMin/+ Mice: Relation to Metabolism and Gut Microbiota Composition. J Cancer Prev 26:32-40 (2021). PubMed: 33842404