AB237540

Anti-ACAA2 抗体

Name: Anti-ACAA2 抗体 (ab237540) | Abcam
Brand: Abcam Limited
SKU: AB237540
Price: 74000 JPY
Availability: InStock

Anti-ACAA2 antibody

(1 Review)

(3 Publications)

Rabbit Polyclonal ACAA2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human 3-ketoacyl-CoA thiolase, mitochondrial aa 150-300.

別名を表示する

Acetyl-CoA acetyltransferase, Acetyl-CoA acyltransferase, Beta-ketothiolase, Mitochondrial 3-oxoacyl-CoA thiolase, T1, ACAA2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA2 antibody (AB237540)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA2 antibody (AB237540)

Paraffin-embedded human kidney tissue stained for ACAA2 using ab237540 at 1/700 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunocytochemistry/ Immunofluorescence - Anti-ACAA2 antibody (AB237540)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ACAA2 antibody (AB237540)

HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for ACAA2 (green) using ab237540 at 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor^®488-conjugated Goat Anti-Rabbit IgG (H+L).

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

IHC-P, ICC/IF, WB

applications

免疫原

Recombinant Fragment Protein within Human 3-ketoacyl-CoA thiolase, mitochondrial aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

P42765

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

精製に関する特記事項

Purity greater than 95%.

バッファー組成

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ACAA2 also known as acetyl-CoA acyltransferase 2 or 3-ketoacyl-CoA thiolase plays an important role in the mechanical process of fatty acid oxidation. It functions in the mitochondria where it catalyzes the final step of the beta-oxidation pathway facilitating the conversion of acetoacetyl-CoA to two molecules of acetyl-CoA. This protein has a known mass of approximately 43 kDa. ACAA2 is expressed in tissues with high energy demands such as the liver heart and muscle highlighting its significance in energy metabolism.

Biological function summary

The enzyme catalyzes reactions in energy metabolism by breaking down fatty acids to generate energy. ACAA2 functions as part of the complex mitochondrial fatty acid beta-oxidation machinery. This process supports energy production particularly under conditions of carbohydrate depletion. The production of acetyl-CoA by ACAA2 is important for the TCA cycle through which cells generate ATP the primary energy carrier.

Pathways

Acetyl-CoA acyltransferase 2 plays significant roles in both the fatty acid beta-oxidation and ketogenesis pathways. In the beta-oxidation pathway it works together with other enzymes like CPT1A and HADHB to efficiently break down fatty acids. In ketogenesis acetyl-CoA units produced by ACAA2 are directed towards the formation of ketone bodies which serve as an alternative energy source for the brain and muscles during fasting or prolonged exercise.

ACAA2 has connections to metabolic disorders particularly fatty acid oxidation disorders and diabetes. Deficiency in ACAA2 activity can result in accumulation and impaired degradation of fatty acids leading to metabolic imbalances. This enzyme also interacts with related proteins such as ACADM in the context of these metabolic diseases. Disruptions in ACAA2 function can face links to mitochondrial dysfunctions that contribute to diabetes due to its role in energy production and regulation.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

In the production of energy from fats, this is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA (Probable). Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain unbranched 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms (Probable). Also catalyzes the condensation of two acetyl-CoA molecules into acetoacetyl-CoA and could be involved in the production of ketone bodies (Probable). Also displays hydrolase activity on various fatty acyl-CoAs (PubMed : 25478839). Thereby, could be responsible for the production of acetate in a side reaction to beta-oxidation (Probable). Abolishes BNIP3-mediated apoptosis and mitochondrial damage (PubMed : 18371312).

See full target information 3-ketoacyl-CoA thiolase, mitochondrial

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Biochimica et biophysica acta. Molecular basis of disease 1868:166476 PubMed35811030

2022

Butyrate oxidation attenuates the butyrate-induced improvement of insulin sensitivity in myotubes.

Applications

Unspecified application

Species

Unspecified reactive species

Melany Rios-Morales,Marcel A Vieira-Lara,Esther Homan,Miriam Langelaar-Makkinje,Albert Gerding,Zhuang Li,Nicolette Huijkman,Patrick C N Rensen,Justina C Wolters,Dirk-Jan Reijngoud,Barbara M Bakker

Frontiers in cell and developmental biology 9:801687 PubMed35096829

2022

Identification and Validation of Key Genes of Differential Correlations in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tingna Chen,Qiuming He,Zhenxian Xiang,Rongzhang Dou,Bin Xiong

International journal of oncology 56:697-708 PubMed31922217

2020

PAX2 promotes epithelial ovarian cancer progression involving fatty acid metabolic reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Feng,Yong Tang,Yannan Mao,Yingzhao Liu,Desheng Yao,Linkai Yang,Kenneth Garson,Barbara C Vanderhyden,Qi Wang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACAA2 抗体

Anti-ACAA2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA2 antibody (AB237540)

Immunocytochemistry/ Immunofluorescence - Anti-ACAA2 antibody (AB237540)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

Abcam product promise