Anti-ACAA1/Beta-ketothiolase 抗体 (ab154091)
Key features and details
- Rabbit polyclonal to ACAA1/Beta-ketothiolase
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ACAA1/Beta-ketothiolase antibody
ACAA1/Beta-ketothiolase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ACAA1/Beta-ketothiolase -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Cow
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免疫原
Recombinant fragment corresponding to Human ACAA1/Beta-ketothiolase aa 50-350.
Database link: P09110 -
ポジティブ･コントロール
- WB: A549, HeLa and mouse liver whole cell lysates; ICC/IF: HeLa cells; IHC-P: Mouse stomach;
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154091の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 44 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| IHC-P |
1/100 - 1/1000.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 44 kDa. |
|
ICC/IF
1/100 - 1/1000. |
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IHC-P
1/100 - 1/1000. |
ターゲット情報
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パスウェイ
Lipid metabolism; fatty acid metabolism. -
配列類似性
Belongs to the thiolase family. -
細胞内局在
Peroxisome. - Information by UniProt
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参照データベース
- Entrez Gene: 508324 Cow
- Entrez Gene: 30 Human
- Entrez Gene: 113868 Mouse
- Omim: 604054 Human
- SwissProt: P09110 Human
- SwissProt: Q921H8 Mouse
- Unigene: 643487 Human
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別名
- 3 ketoacyl CoA thiolase peroxisomal antibody
- 3-ketoacyl-CoA thiolase antibody
- ACAA antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody (ab154091)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse stomach staining ACAA1/Beta-ketothiolase with ab154091 at a dilution of 1/500.
EDTA based Antigen Retrieval, pH 8.0 for 15min.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody (ab154091)Immunofluorescent analysis of methanol-fixed HeLa cells labeling ACAA1/Beta-ketothiolase with ab154091 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
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Western blot - Anti-ACAA1/Beta-ketothiolase antibody (ab154091)All lanes : Anti-ACAA1/Beta-ketothiolase antibody (ab154091) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 44 kDa
10% SDS PAGE -
Western blot - Anti-ACAA1/Beta-ketothiolase antibody (ab154091)Anti-ACAA1/Beta-ketothiolase antibody (ab154091) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 44 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-ACAA1/Beta-ketothiolase antibody (ab154091)Immunofluorescent analysis of methanol-fixed HeLa cells labeling ACAA1/Beta-ketothiolase with ab154091 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab154091 は 1 報の論文で使用されています。
- Colasuonno F et al. Mitochondrial and Peroxisomal Alterations Contribute to Energy Dysmetabolism in Riboflavin Transporter Deficiency. Oxid Med Cell Longev 2020:6821247 (2020). PubMed: 32855765