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リコンビナントRabMAb

Anti-Abhd5/CGI-58 抗体 [EPR12621] (ab183739)

Reviews (2) Submit a question References (21)
Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
  • Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
  • Flow Cytometry (Intracellular) - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
  • Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12621] to Abhd5/CGI-58
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC Carrier Free HRP PE

製品の概要

  • 製品名

    Anti-Abhd5/CGI-58 antibody [EPR12621]
    Abhd5/CGI-58 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR12621] to Abhd5/CGI-58

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-Pmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HepG2 cells and cell lysates; U87-MG and Human fetal liver cell lysates; Human lipomyoma tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab183739の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (2)
1/1000 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).
IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
Flow Cyt (Intra)
1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).
IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation.

  • 組織特異性

    Widely expressed in various tissues, including lymphocytes, liver, skeletal muscle and brain. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and neurons (at protein level).

  • 関連疾患

    Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS) [MIM:275630]; also called triglyceride storage disease with impaired long-chain fatty acid oxidation or neutral lipid storage disease with ichthyosis. CDS is an autosomal recessive inborn error of lipid metabolism with multisystemic accumulation of triglycerides although plasma concentrations are normal. Clinical characteristics are congenital generalized ichthyosis, vacuolated leukocytes, hepatomegaly, myopathy, cataracts, neurosensory hearing loss and developmental delay. The disorder presents at birth with generalized, fine, white scaling of the skin and a variable degree of erythema resembling non-bullous congenital ichthyosiform erythroderma.

  • 配列類似性

    Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.

  • 発生段階

    Detected in fetal epidermis from 49 to 135 days estimated gestational age (at protein level).

  • ドメイン

    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.

  • 細胞内局在

    Cytoplasm. Lipid droplet. Colocalized with PLIN and ADRP on the surface of lipid droplets. The localization is dependent upon the metabolic status of the adipocytes and the activity of PKA.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 1-acylglycerol-3-phosphate O-acyltransferase ABHD5 antibody
      • ABHD5 antibody
      • ABHD5_HUMAN antibody
      • Abhydrolase domain containing 5 antibody
      • Abhydrolase domain containing protein 5 antibody
      • Abhydrolase domain-containing protein 5 antibody
      • CDS antibody
      • CGI 58 antibody
      • CGI58 antibody
      • CGI58 protein antibody
      • IECN2 antibody
      • Lipid droplet-binding protein CGI-58 antibody
      • MGC8731 antibody
      • NCIE2 antibody
      see all

    画像

    • Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
      Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
      All lanes : Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : U87-MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 39 kDa
      Observed band size: 45 kDa why is the actual band size different from the predicted?



      PMID: 28211464, PMID: 19801371, PMID: 18606822, PMID: 18832586.

    • Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
      Western blot - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
      Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739) at 1/1000 dilution + Human fetal liver lysate at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 39 kDa
      Observed band size: 45 kDa why is the actual band size different from the predicted?



      PMID: 28211464, PMID: 19801371, PMID: 18606822, PMID: 18832586.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)

      Immunohistochemical analysis of paraffin-embedded Human lipomyoma tissue labeling Abhd5/CGI-58 with ab183739 at 1/2000 dilution. The slide is counterstained with Hematoxylin.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
      Flow Cytometry (Intracellular) - Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)

      Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 cells labeling Abhd5/CGI-58 with ab183739 at 1/200 dilution. Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control. 

       

       

    • Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)
      Anti-Abhd5/CGI-58 antibody [EPR12621] (ab183739)

    参考文献 (21)

    ab183739 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab183739 は 21 報の論文で使用されています。

    • Hara M  et al. PNPLA2 mobilizes retinyl esters from retinosomes and promotes the generation of 11-cis-retinal in the visual cycle. Cell Rep 42:112091 (2023). PubMed: 36763501
    • Qian K  et al. CLSTN3β enforces adipocyte multilocularity to facilitate lipid utilization. Nature 613:160-168 (2023). PubMed: 36477540
    • Ahn C  et al. Exercise training remodels subcutaneous adipose tissue in adults with obesity even without weight loss. J Physiol 600:2127-2146 (2022). PubMed: 35249225
    • Zhou M  et al. Lipidomic analysis reveals altered lipid profiles of gingival tissues with periodontitis. J Clin Periodontol 49:1192-1202 (2022). PubMed: 35924763
    • Su W  et al. Phosphorylation of 17β-hydroxysteroid dehydrogenase 13 at serine 33 attenuates nonalcoholic fatty liver disease in mice. Nat Commun 13:6577 (2022). PubMed: 36323699
    View all Publications for this product

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-Abhd5/CGI-58 antibody [EPR12621]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (white adipose tissue)
    Gel Running Conditions
    Reduced Denaturing (7.5%)
    Loading amount
    5 µg
    Specification
    white adipose tissue
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jul 25 2022

    Western blot abreview for Anti-Abhd5/CGI-58 antibody [EPR12621]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (adipose tissue)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    adipose tissue
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Ms. Helle Zibrandtsen

    Verified customer

    投稿 Mar 11 2015

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