Anti-ABCF2 抗体 [2001C1] (ab50807)
![Western blot - Anti-ABCF2 antibody [2001C1] (ab50807)](https://www.abcam.co.jp/ps/products/50/ab50807/Images/ab50807-414934-anti-abcf2-antibody-2001c1-western-blot-human-knockout-lysate.jpg "Western blot - Anti-ABCF2 antibody [2001C1] (ab50807)")
Key features and details
- Mouse monoclonal [2001C1] to ABCF2
- Suitable for: WB
- Knockout validated
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
製品の概要
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製品名
Anti-ABCF2 antibody [2001C1] -
製品の詳細
Mouse monoclonal [2001C1] to ABCF2 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Recombinant full length protein corresponding to Human ABCF2.
Database link: Q9UG63 -
ポジティブ･コントロール
- WB: HEK293 and HeLa cell lysates; Recombinant ABCF2 fragment.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.0225% Potassium chloride, 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
ab50807 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22 µm membrane. -
ポリ/モノ
モノクローナル -
クローン名
2001C1 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50807の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 71 kDa.
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| 特記事項 |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 71 kDa. |
ターゲット情報
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関連性
ABCF 2 is a member of the ABC Transporter superfamily and of the GCN20 subfamily. As a member of the ABC Transporter family, it functions to transport various molecules across extra- and intracellular membranes. -
参照データベース
- Entrez Gene: 10061 Human
- Omim: 612510 Human
- SwissProt: Q9UG63 Human
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別名
- 0710005O05Rik antibody
- ABC28 antibody
- ABCF 2 antibody
see all
画像
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Western blot - Anti-ABCF2 antibody [2001C1] (ab50807)All lanes : Anti-ABCF2 antibody [2001C1] (ab50807) at 1/500 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : ABCF2 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 71 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab50807 observed at 75 kDa. Red - loading control ab181602 observed at 36 kDa.
ab50807 Anti-ABCF2 antibody [2001C1] was shown to specifically react with ABCF2 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266516 (knockout cell lysate ab258773) was used. Wild-type and ABCF2 knockout samples were subjected to SDS-PAGE. ab50807 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-ABCF2 antibody [2001C1] (ab50807)](https://www.abcam.co.jp/ps/products/50/ab50807/Images/ab50807 copy.gif)
Western blot - Anti-ABCF2 antibody [2001C1] (ab50807)Anti-ABCF2 antibody [2001C1] (ab50807) + Recombinant ABCF2 fragment
Predicted band size: 71 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (0)
ab50807 は論文での使用が確認できていません。
