Anti-ABCE1 抗体 [EPR15373(B)] - C-terminal

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (AB185548)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling ABCE1 with Purified ab185548 at 1 : 100 (11 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (AB185548)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling ABCE1 with Purified ab185548 at 1 : 100 (11 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (AB185548)

Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labelling ABCE1 with Purified ab185548 at 1 : 100 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1 : 2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (AB185548)

Immunocytochemistry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling ABCE1 with Purified ab185548 at 1 : 100 dilution (10 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A]+H21 : L21 - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (AB185548)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling ABCE1 with Purified ab185548 at 1 : 300 (3.67 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (AB185548)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue sections labeling ABCE1 with Purified ab185548 at 1 : 300 (3.67 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

• WB

Unknown

Western blot - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (AB185548)

All lanes:

Western blot - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548) at 1/5000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

Lane 2:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate

Lane 3:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

Lane 5:

PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 67 kDa

false

• WB

CiteAb

Western blot - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (AB185548)

ABCE1 western blot using anti-ABCE1 antibody [EPR15373(B)] - C-terminal ab185548. Publication image and figure legend from Yin, H., Jiang, Z., et al., 2019, Cell Death Dis, PubMed 31501431.

ab185548 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab185548 please see the product overview.

Upregulation of RNase L by IFN-γ neutralized RLI and restored cleavage activity.a, b Dose dependence (a) and time course (b) of IFN-γ in restoring the cleavage activity of RNase L (n = 3). c Cleavage activity of RNase L in the presence of IFN-γ in BEAS-2B and NCI-H157 cells (n = 3). d Western blot of OAS1, OAS2, and OAS3 in the presence of IFN-γ in BEAS-2B and NCI-H157 cells. e, f Western blot verification and rRNA cleavage after OAS3 interference by siRNA; g, j Western blot of RNase L dimer or monomer in the cytoplasm (g) or nucleus (j) in the presence of IFN-γ in BEAS-2B and NCI-H157 cells. h, i, k, l Cytoplasmic (h, i) and nuclear (k, l) RLI expression (i, l) and IP with RNase L (h, k) in the presence of IFN-γ in BEAS-2B and NCI-H157 cells

false