Anti-ABCB6/PRP 抗体 [EPR21891] (ab221159)
![Flow Cytometry (Intracellular) - Anti-ABCB6/PRP antibody [EPR21891] (ab221159)](https://www.abcam.co.jp/ps/products/221/ab221159/Images/ab221159-312403-anti-abcb6-antibody-epr21891-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Anti-ABCB6/PRP antibody [EPR21891] (ab221159)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21891] to ABCB6/PRP
- Suitable for: Flow Cyt (Intra), ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ABCB6/PRP antibody [EPR21891]
ABCB6/PRP 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR21891] to ABCB6/PRP -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- ICC/IF: HeLa and K562 cells. Flow: HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR21891 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab221159の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
|
|
| ICC/IF |
1/500.
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/500. |
|
ICC/IF
1/500. |
ターゲット情報
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機能
Binds heme and porphyrins and functions in their ATP-dependent uptake into the mitochondria. Plays a crucial role in heme synthesis. -
組織特異性
Widely expressed. Highest expression in heart and skeletal muscles. -
配列類似性
Belongs to the ABC transporter superfamily. ABCB family. Heavy Metal importer (TC 3.A.1.210) subfamily.
Contains 1 ABC transmembrane type-1 domain.
Contains 1 ABC transporter domain. -
発生段階
Highly expressed in fetal liver. -
細胞内局在
Mitochondrion outer membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 10058 Human
- Omim: 605452 Human
- SwissProt: Q9NP58 Human
- Unigene: 107911 Human
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別名
- ABC antibody
- ABC transporter umat antibody
- ABC14 antibody
see all
画像
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Flow Cytometry (Intracellular) - Anti-ABCB6/PRP antibody [EPR21891] (ab221159)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling ABCB6/PRP with ab221159 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-ABCB6/PRP antibody [EPR21891] (ab221159)](https://www.abcam.co.jp/ps/products/221/ab221159/Images/ab221159-312402-anti-abcb6-antibody-epr21891-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ABCB6/PRP antibody [EPR21891] (ab221159)Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized K-562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling ABCB6/PRP with ab221159 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing lysosome staining in K-562 cell line.
The nuclear counter stain is DAPI (blue). Counterstained with ab25630 anti-LAMP1 (mouse mAb) at 100 dilution and ab150120 AlexaFluor®594 Goat anti-Mouse secondary at 1/1000 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-ABCB6/PRP antibody [EPR21891] (ab221159)](https://www.abcam.co.jp/ps/products/221/ab221159/Images/ab221159-312401-anti-abcb6-antibody-epr21891-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ABCB6/PRP antibody [EPR21891] (ab221159)Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ABCB6/PRP with ab221159 at 1/500 dilution, followed by ab150077 AlexaFluorGoat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing lysosome staining in K-562 cell line.
The nuclear counter stain is DAPI (blue). Counterstained with ab25630 anti-LAMP1 (mouse mAb) at 100 dilution and ab150120 AlexaFluor®594 Goat anti-Mouse secondary at 1/1000 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Anti-ABCB6/PRP antibody [EPR21891] (ab221159)](https://www.abcam.co.jp/ps/products/221/ab221159/Images/ab221159-4-benefits-of-recombinant-antibodies.png)
Anti-ABCB6/PRP antibody [EPR21891] (ab221159)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab221159 は論文での使用が確認できていません。