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Anti-ABCB4 抗体 [P3II-26] (ab24108)

Submit a review Q&A (2)References (6)
Flow Cytometry - Anti-ABCB4 antibody [P3II-26] (ab24108)

    Key features and details

    • Mouse monoclonal [P3II-26] to ABCB4
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG2b

    製品の概要

    • 製品名

      Anti-ABCB4 antibody [P3II-26]
      ABCB4 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [P3II-26] to ABCB4

    • 由来種

      Mouse

    • 特異性

      Clone P3II-26 does not cross-react with the human ABCB1.

    • アプリケーション

      適用あり: Flow Cytmore details
      適用なし: IHC-P

    • 種交差性

      交差種: Human

    • 免疫原

      Recombinant fragment within Human ABCB4 aa 600-700 (internal sequence). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • エピトープ

      Clone P3II-26 reacts with an internal epitope of ABCB4.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab24108の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Flow Cyt
    Use 1µg for 106 cells.

    ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. 
    特記事項
    Flow Cyt
    Use 1µg for 106 cells.

    ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. 
    追加情報
    Is unsuitable for IHC-P.

    ターゲット情報

    • 機能

      Mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Human MDR3 is not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte.

    • 関連疾患

      Defects in ABCB4 are the cause of progressive familial intrahepatic cholestasis type 3 (PFIC3) [MIM:602347]. PFIC3 is an autosomal recessive liver disorder presenting with early onset cholestasis that progresses to cirrhosis and liver failure before adulthood. It is characterized by elevated serum gamma-glutamyltransferase levels.
      Defects in ABCB4 are a cause of intrahepatic cholestasis of pregnancy (ICP) [MIM:147480]; also known as obstetric cholestasis. ICP is a multifactorial liver disorder of pregnancy. It presents during the second or, more commonly, the third trimestre of pregnancy with intense pruritus which becomes more severe with advancing gestation and cholestasis. Cholestasis results from abnormal biliary transport from the liver into the small intestine. ICP causes fetal distress, spontaneous premature delivery and intrauterine death. ICP patients have spontaneous and progressive disappearance of cholestasis after delivery.
      Defects in ABCB4 are a cause of gallbladder disease type 1 (GBD1) [MIM:600803]. It is one of the major digestive diseases. Gallstones composed of cholesterol (cholelithiasis) are the common manifestations in western countries. Most people with gallstones, however, remain asymptomatic through their lifetimes.

    • 配列類似性

      Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
      Contains 2 ABC transmembrane type-1 domains.
      Contains 2 ABC transporter domains.

    • 細胞内局在

      Cell membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • ABC 21 antibody
        • ABC B4 antibody
        • ABC21 antibody
        • ABCB 4 antibody
        • Abcb4 antibody
        • ABCB4 protein antibody
        • ATP binding cassette sub family B MDR/TAP member 4 antibody
        • ATP binding cassette sub family B member 4 antibody
        • ATP-binding cassette sub-family B member 4 antibody
        • GBD1 antibody
        • ICP3 antibody
        • MDR 3 antibody
        • MDR2 antibody
        • MDR2/3 antibody
        • MDR3 antibody
        • MDR3 P glycoprotein antibody
        • MDR3 P gp antibody
        • MDR3_HUMAN antibody
        • Multidrug resistance protein 3 antibody
        • Multiple drug resistance 3 antibody
        • P glycoprotein 3 antibody
        • P-glycoprotein 3 antibody
        • PFIC 3 antibody
        • PFIC3 antibody
        • PGY 3 antibody
        • PGY3 antibody
        see all

      画像

      • Flow Cytometry - Anti-ABCB4 antibody [P3II-26] (ab24108)
        Flow Cytometry - Anti-ABCB4 antibody [P3II-26] (ab24108)
        Overlay histogram showing HeLa cells stained with ab24108 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24108, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

      参考文献 (6)

      ab24108 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab24108 は 6 報の論文で使用されています。

      • Hong X  et al. Prostate-specific membrane antigen modulates the progression of prostate cancer by regulating the synthesis of arginine and proline and the expression of androgen receptors and Fos proto-oncogenes. Bioengineered 13:995-1012 (2022). PubMed: 34974814
      • Wen C  et al. Curcumin reverses doxorubicin resistance via inhibition the efflux function of ABCB4 in doxorubicin-resistant breast cancer cells. Mol Med Rep 19:5162-5168 (2019). PubMed: 31059026
      • Gordillo GM  et al. Multidrug Resistance-associated Protein-1 (MRP-1)-dependent Glutathione Disulfide (GSSG) Efflux as a Critical Survival Factor for Oxidant-enriched Tumorigenic Endothelial Cells. J Biol Chem 291:10089-103 (2016). PubMed: 26961872
      • Gordillo GM  et al. Multidrug resistance-associated protein-1 (MRP-1)-dependent glutathione disulfide (GSSG) efflux as a critical survival factor for oxidant-enriched tumorigenic endothelial cells. J Biol Chem 291:14394 (2016). PubMed: 27371570
      • Bachour-El Azzi P  et al. Comparative Localization and Functional Activity of the Main Hepatobiliary Transporters in HepaRG Cells and Primary Human Hepatocytes. Toxicol Sci 145:157-68 (2015). PubMed: 25690737
      • Scheffer GL  et al. Specific detection of multidrug resistance proteins MRP1, MRP2, MRP3, MRP5, and MDR3 P-glycoprotein with a panel of monoclonal antibodies. Cancer Res 60:5269-77 (2000). PubMed: 11016657

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      1-2 of 2 Abreviews or Q&A

      Question

      Dear Sir/Madam, I was recently reading an article which uses one of your antibodies. I cannot seem to locate this product on your website and was wondering if you could point me in the right direction. The article states using: "a mouse-monoclonal antibody against mammalian Pgp (C219; Abcam, Cambridge, MA, USA).... This antibody targets two Pgp epitopes that are largely conserved in the SMDR2 sequence" Another issue you may be able to help me with is whether or not you have a primary antibody that would recognise a variety of pgp/mdr proteins (has to include MDR2 (human gene family ABCB1) and MRP1 (human gene family ABCC1)) i.e. it may bind a highly conserved region across the pgps. I would really appreciate any help with the matter as I have been trawling through many of the antibodies available and comparing immunogens. I hope you can help, thank you for your time Kind regards

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      Answer

      Thank you for contacting us. ab3364; anti P Glycoprotein is no longer available in our catalogue. However we have similar products available to buy. The catalogue numbers are ab3083; ab3366; ab10333; ab80594; ab80626. Please check the datasheets of these antibodies and select a product according to your requirements. The anti MDR2 products are ab71792; ab24108 and ab111209 Anti MRP1 products are ab24102; ab63987; ab99531; ab81903 To search different products please type target name in the search box provided on the Abcam homepage at www.abcam.com. After hitting the search button the system will give you the sorted product as per your target request. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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      Question

      I will need to detect MDR3 protein in a stably transfected cell line by performing western blotting. I am considering to use P3II-26 (product No. ab24108) as the primary antibody. I wonder if you could help me with a couple of questions: 1. Did you test this antibody for use in a western blot application? 2. If yes, what did you use as the positive control and what's the dilution for this antibody?

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      Answer

      Thank you for your enquiry. This antibody was tested in Western blot. I could not find a specific dilution recommended for this antibody, but in general we recommend using 1-20ug/ml of primary antibody, and this antibody is supplied as approximately 250 µg immunoglobulin/ml. Therefore, I would recommend starting at 1:100 and optimizing from there. I would recommend liver tissue as a positive control, since the datasheet states that this protein mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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