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遺伝子ノックアウト細胞株 検証済

Anti-ABCA1 抗体 [AB.H10] (ab18180)

Reviews (18)Q&A (36)References (208)
Flow Cytometry - Anti-ABCA1 antibody [AB.H10] (ab18180)
  • Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180)
  • Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [AB.H10] (ab18180)

Key features and details

  • Mouse monoclonal [AB.H10] to ABCA1
  • Suitable for: IHC-P, Flow Cyt, WB
  • Knockout validated
  • Reacts with: Mouse, Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-ABCA1 antibody [AB.H10]
    ABCA1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [AB.H10] to ABCA1

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-P, Flow Cyt, WBmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Recombinant fragment corresponding to Human ABCA1 aa 1800-2260.
    Database link: O95477

  • ポジティブ･コントロール

    • testis, liver, and brain tissue (negative control: muscle tissue)

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab18180の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt (3)
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB (13)
1/200 - 1/500. Predicted molecular weight: 254 kDa.

Abcam recommends using BSA as the blocking agent and 80-100 ug of lysate.
特記事項
IHC-P
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
1/200 - 1/500. Predicted molecular weight: 254 kDa.

Abcam recommends using BSA as the blocking agent and 80-100 ug of lysate.

ターゲット情報

  • 機能

    cAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport.

  • 組織特異性

    Widely expressed, but most abundant in macrophages.

  • 関連疾患

    Defects in ABCA1 are a cause of high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by absence of high density lipoprotein (HDL) cholesterol from plasma, accumulation of cholesteryl esters, premature coronary artery disease (CAD), hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness.
    Defects in ABCA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). HDLD2 is inherited as autosomal dominant trait. It is characterized by moderately low HDL cholesterol, predilection toward premature coronary artery disease (CAD) and a reduction in cellular cholesterol efflux.

  • 配列類似性

    Belongs to the ABC transporter superfamily. ABCA family.
    Contains 2 ABC transporter domains.

  • ドメイン

    Multifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.

  • 翻訳後修飾

    Phosphorylation on Ser-2054 regulates phospholipid efflux.
    Palmitoylation by DHHC8 is essential for membrane localization.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ABC 1 antibody
      • ABC Transporter 1 antibody
      • ABC-1 antibody
      • ABC1 antibody
      • ABCA 1 antibody
      • ABCA1 antibody
      • ABCA1_HUMAN antibody
      • ATP binding Cassette 1 antibody
      • ATP binding cassette sub family A ABC1 member 1 antibody
      • ATP binding cassette sub family A member 1 antibody
      • ATP binding Cassette Transporter 1 antibody
      • ATP-binding cassette 1 antibody
      • ATP-binding cassette sub-family A member 1 antibody
      • ATP-binding cassette transporter 1 antibody
      • CERP antibody
      • Cholesterol efflux regulatory protein antibody
      • FLJ14958 antibody
      • HDLDT1 antibody
      • Membrane bound antibody
      • MGC164864 antibody
      • MGC165011 antibody
      • TD antibody
      • TGD antibody
      see all

    画像

    • Flow Cytometry - Anti-ABCA1 antibody [AB.H10] (ab18180)
      Flow Cytometry - Anti-ABCA1 antibody [AB.H10] (ab18180)
      Overlay histogram showing HepG2 cells stained with ab18180 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18180, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

    • Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180)
      Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180)
      Western blot analysis of ABCA1 induction by cholesterol (+) in human fibroblast cells from different patients (1,2,3,4). Simultaneous bloting with anti-actin antibody was used for protein loading control. Western blot analysis of ABCA1 induction by cholesterol (+) in human fibroblast cells from different patients (1,2,3,4). Simultaneous blotting with anti-actin antibody was used for protein loading control.
    • Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180)
      Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180)
      All lanes : Anti-ABCA1 antibody [AB.H10] (ab18180) at 1/1000 dilution

      Lanes 1 & 3 & 5 : Wild type mouse liver tissue lysate
      Lanes 2 & 4 & 6 : Knockout mouse liver tissue lysate

      Lysates/proteins at 100 µg per lane.

      Predicted band size: 254 kDa
      Observed band size: 254 kDa



      The lower bands are from the secondary anti-mouse antibody reacting to the endogenous mouse antibodies from the tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [AB.H10] (ab18180)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [AB.H10] (ab18180)
      Immunoreactivity of pancreas tissue for ABCA1 protein. Left panel: strong immunopositivity of exocrine glandular cells of the pancreas, especially in the basal region (arrows). Interstitial microvascular cells are apparently negative. Cells within the islet were weakly positive (data not shown). Right panel: the negative control. Hematoxylin counterstain.

    参考文献 (208)

    ab18180 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab18180 は 208 報の論文で使用されています。

    • Hong Y  et al. Genetic dissection of the impact of lncRNA AI662270 during the development of atherosclerosis. J Transl Med 21:97 (2023). PubMed: 36755320
    • Zhang X  et al. Effects of Caprylic Acid and Eicosapentaenoic Acid on Lipids, Inflammatory Levels, and the JAK2/STAT3 Pathway in ABCA1-Deficient Mice and ABCA1 Knock-Down RAW264.7 Cells. Nutrients 15:N/A (2023). PubMed: 36904298
    • An HJ  et al. STAT3/NF‑κB decoy oligodeoxynucleotides inhibit atherosclerosis through regulation of the STAT/NF‑κB signaling pathway in a mouse model of atherosclerosis. Int J Mol Med 51:N/A (2023). PubMed: 37026512
    • George M  et al. Liver X Receptor Activation Attenuates Oxysterol-Induced Inflammatory Responses in Fetoplacental Endothelial Cells. Cells 12:N/A (2023). PubMed: 37190095
    • Zhou J  et al. Macrophage Gpx4 deficiency aggravates foam cell formation by regulating the expression of scavenger receptors, ABCA1, and ABCG1. Cell Biol Int 47:1589-1599 (2023). PubMed: 37309064
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 54 Abreviews or Q&A

    Western blot abreview for Anti-ABCA1 antibody [AB.H10]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Guinea pig Tissue lysate - other (Brain membrane)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    20 µg
    Specification
    Brain membrane
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Dec 02 2022

    Western blot abreview for Anti-ABCA1 antibody [AB.H10]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Sheep Cell lysate - whole cell (Ovine Thecal Cells)
    Gel Running Conditions
    Reduced Denaturing (10% PAG)
    Loading amount
    10 µg
    Specification
    Ovine Thecal Cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MISS. Barbara Makela

    Verified customer

    投稿 Jun 22 2017

    Western blot abreview for Anti-ABCA1 antibody [AB.H10]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver)
    Gel Running Conditions
    Reduced Denaturing (7.5)
    Loading amount
    25 µg
    Specification
    liver
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Nov 24 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ABCA1 antibody [AB.H10]

     Average
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Primary astrocyte culture)
    Permeabilization
    Yes - 0.1M PBS with 1% Triton X
    Specification
    Primary astrocyte culture
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Methanol
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Ruma Raha-Chowdhury

    Verified customer

    投稿 Sep 06 2016

    Western blot abreview for Anti-ABCA1 antibody [AB.H10]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rabbit Tissue lysate - whole (Synovial membrane)
    Gel Running Conditions
    Reduced Denaturing (8 %)
    Loading amount
    35 µg
    Specification
    Synovial membrane
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jun 13 2016

    Flow Cytometry abreview for Anti-ABCA1 antibody [AB.H10]

     Good
    Abreviews
    Application
    Flow Cytometry
    Fixation
    Formaldehyde
    Permeabilization
    Yes - BD PERMEABILISATION SOLUTION
    Sample
    Mouse Cell (liver cells)
    Specification
    liver cells
    Gating Strategy
    CD24 POSITIVE
    Preparation
    Cell harvesting/tissue preparation method: single cell suspension
    Sample buffer: 0.1%PBS-BSA
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Dec 10 2013

    Flow Cytometry abreview for Anti-ABCA1 antibody [AB.H10]

     Good
    Abreviews
    Application
    Flow Cytometry
    Fixation
    Formaldehyde
    Permeabilization
    Yes - BD Permeabilisation Solution
    Sample
    Rat Cell (Liver cells)
    Specification
    Liver cells
    Gating Strategy
    CD24 Positive
    Preparation
    Cell harvesting/tissue preparation method: Single cell suspension
    Sample buffer: 0.1% PBS-BSA
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Dec 05 2013

    Flow Cytometry abreview for Anti-ABCA1 antibody [AB.H10]

     Good
    Abreviews
    Application
    Flow Cytometry
    Fixation
    Formaldehyde
    Permeabilization
    Yes - BD PERMEABILISATION SOLUTION
    Sample
    Human Cell (HEP G2)
    Specification
    HEP G2
    Gating Strategy
    CD24 POSITIVE
    Preparation
    Cell harvesting/tissue preparation method: single cell suspension
    Sample buffer: 0.1%PBS-BSA
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. brahmi manali

    Verified customer

    投稿 Dec 04 2013

    Western blot abreview for Anti-ABCA1 antibody [AB.H10]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Pig Tissue lysate - whole (small intestine)
    Loading amount
    30 µg
    Specification
    small intestine
    Gel Running Conditions
    Reduced Denaturing (7)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Dongil Kim

    Verified customer

    投稿 Apr 23 2013

    Question

    Thank you for your kind replacement of the antibody! I was able to test it on human prostate cancer cell line PC3 lysed with RIPA buffer (last lane of the samples indicated by arrow) and my other prostate cancer cell lines prepared before. The membrane was first detected with your new antibody and then reprobed with FASN. As you can see from the images, your new antibody can only show a low molecular weight background band. FASN antibody clearly detects the target protein with several non-specific bands. The pictures in two different pages represents different exposure time. Therefore, I am afraid that your new antibody does not work either. If you can provide a positive control, I am willing to repeat experiment again. If your positive control works, it means that your antibodies work. I am happy to keep them and improve my sample preparation.

    Read More
    Answer


    Yes, I can send you as a one time exception a positive control lysate (rat liver (ab29413), https://www.abcam.com/liver-rat-whole-cell-lysate-normal-tissue-ab29413.html). This has been tested with ab66217 and gave strong bands.

    Also I would suggest to check the gel after transfer as some of the high MW proteins might have not completely transfered onto the membrane. You can check this by staining the gel with Coomassie Blue. if you see bands in the gel at 200 kDa and higher, you might want to adjust your transfer conditions so that all high MW protein are being transfered.

    Also, avoid boiling of your samples before loading them onto the gel. Warming your sample to 50-60 degree Celsius is sufficient for denaturation in loading buffer.

    Again, FASN is a cytoplasmic protein and such is much easier to work with than ABCA1 which is very hydrophobic, glycosylated and of high MW.

    This article and link might be of help as well: http://www.sciencedirect.com/science/article/pii/S0003269708006829
    http://www.scientistsolutions.com/t14685-membrane+protein+western+blot.html

    Read More

    1-10 of 54 Abreviews or Q&A

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